Dispersion and stabilization of cochleate nanoparticles

Abstract

Cochleates, calcium-stabilized membrane rolls of nanoscale diameter, promise a unique and efficient way of delivering lipid-soluble drugs, proteins or nucleic acids into biological systems because they protect the encapsulated material against enzymatic or chemical degradation. Self-aggregation, which typically arises during production and storage is a major obstacle that has so far precluded the development of an efficient cochleate-based drugdelivery system. Here we show that citric acid, added transiently in a narrow concentration range, effectively disperses cochleate aggregates, stabilizes the disperse state for long-term storage and preserves the canonical ultrastructure and topological characteristics of cochleate nanoparticles.

Cochleate formulations of Amphotericin b designed for oral administration using a naturally occurring phospholipid

“…Furthermore, the observed particles are in the nanometer range, in agreement with what would be expected by the hydrogel isolation method to obtain nanocochleates (Jin et al, 2000). Images A(2), B(2), C(2) and D(2) obtained by optical microscopy shows some larger cochleates, (black arrows), maybe due to the presence of other lipid species in the naturally occurring phospholipid or to the variability of the acyl chains of the phosphatidylserine; leading to less ordered structure, but which could also be caused by calcium ion excess, a serious drawback reported in the fabrication of cochleates where excess calcium can provoke fusion between cochleates (Bozó et al, 2017).…”

Recent strategies in nanodelivery systems for natural products: a review

“…They are characterized by an exceptional stability due to their cylindrical shape and distinctive multi-layered structure (Fig. 2) (Bozó et al 2017;Tilawat & Bonde 2021) and they were studied to formulate different kind of natural products.…”

<p>Oral Nano Drug Delivery Systems for the Treatment of Type 2 Diabetes Mellitus: An Available Administration Strategy for Antidiabetic Phytocompounds</p>

“…Compared with native resveratrol, the lower dose of nanocochleates loaded with resveratrol better improved the decreased insulin levels rendered with glucose and STZ, markedly reduced the increased glucose levels, and also exhibited prolonged antioxidant activity for 24 h. However, self-aggregation, which usually happens during production and storage, is currently the major obstacle hindering the development of efficient cochleate-based drug delivery systems. 207…”