Micro-Sized Glucose Biosensor Based On Composite pH Sensor

Abstract

ÖzToday's scientific and technical advances have led to significant advances in biosensor technology, leading to widespread use of biosensors in areas such as food technology, the environment, pharmaceuticals and clinical diagnostics, biochemistry, and analytical chemistry. Improvements in the ion-selective sensor technology used in the preparation of potentiometric biosensors and the increase in the number of sensor materials have also accelerated the development of new types of potentiometric biosensors. Thus, in this study, information is given on the development of a new micro sized potentiometric glucose biosensor based on composite pH sensor. Glucose oxidase was immobilized on the composite layer as the biorecognition element. The surface of glucose biosensor based composite pH sensing matrices was first examined for electrochemical elucidation by using cyclic voltammetry and electrochemical impedance spectroscopy. The rate of electron transfer resulted in a stable response behavior of the biosensor and made it a suitable potentiometric sensor for detection of glucose. A linear response in concentration range from 5x10-5 to 1x10 -1 mol/L was obtained with a detection limit of 2x10 -5 mol/L for the potentiometric detection of glucose. The biosensor exhibited a fast response time (10 s), had good stability, and an extended lifetime.

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“…are functions of product quality indicators that can be directly measured using smart sensors and calculated using mathematical models based on neural network or expert system technology, which can infer the quality of the product. In the field of medicine [10], smart sensors were integrated into glucose watches using composite pH-sensitive sensors, and a new glucosesensitive potentiometric biosensor was developed using composite pH-sensitive sensors, characterized by EIS and CV measurements. In particular, with the rapid development of semiconductor technology in recent years, several well-known foreign companies and institutions of higher education are making great efforts to develop the integration of smart sensors.…”