Micro RNA (miR)‐203 and miR‐205 expression patterns identify subgroups of prognosis in cutaneous squamous cell carcinoma

Abstract

Cutaneous squamous cell carcinoma is the second most widespread cancer in humans and its incidence is rising. These tumours can evolve as poor-prognosis diseases, and therefore it is important to identify new markers to better predict its clinical evolution. Here, we identified the expression pattern of miRNAs at different stages of skin cancer progression in a panel of murine skin cancer cell lines. We determined that miR-203 and miR-205 are differentially expressed in this panel, and evaluated their potential use as biomarkers of prognosis in human tumours. MiR-205 was expressed in tumours with pathological features recognized as indicators of poor prognosis such as desmoplasia, perineural invasion and infiltrative growth pattern. MiR-205 was mainly expressed in undifferentiated areas and in the invasion front, and was associated with both local recurrence and the development of general clinical events of poor evolution. MiR-205 expression was an independent variable selected to predict events of poor clinical evolution using the multinomial logistic regression model described in this study. In contrast, miR-203 was mainly expressed in tumours exhibiting the characteristics associated with a good prognosis, was mainly present in well-differentiated zones, and rarely expressed in the invasion front. Therefore, the expression and associations of miR-205 and miR-203 were mostly mutually exclusive. Finally, using a logistic biplot we identified three clusters of patients with differential prognosis based on miR-203 and miR-205 expression, and pathological tumour features. This work highlights the utility of miR-205 and miR-203 as prognostic markers in cutaneous squamous cell carcinoma.

A novel six-gene signature for prognosis prediction in ovarian cancer

“…Robust biomarkers are emerging to stratify high-risk groups among these patients. For example, the expression level of miR-205 was an independent variation selected to predict the events of poor clinical evolution [16]. The soluble VEGFR-2 level could predict the malignancy of ovarian neoplasms and poor prognosis in epithelial OC [17].…”

MicroRNA‑186 promotes cell proliferation and inhibits cell apoptosis in cutaneous squamous cell carcinoma by targeting RETREG1

“…Furthermore, the expression of RETREG1 was negatively regulated by miR-186 in CSCC cells, and restoration of RETREG1 attenuated the effects of miR-186 on CSCC cells. In recent decades, a large number of miRs, including, miR-125b (11), miR-365 (20), miR-205 (21), miR-199a-5p (22), and miR-203 (23) have been identified as key regulators of the development and progression of CSCC and numerous other types of human cancer. For instance, miR-199a-5p induces CSCC cell invasion by suppressing E-cadherin expression (22).…”

miR-205 regulates the proliferation and invasion of ovarian cancer cells via suppressing PTEN/SMAD4 expression

“…Inhibition of miR-205 expression using LNA-modified-nucleotide attenuated endometrial cancer cells proliferation in vitro and in vivo ( 20 ). miR-205 was expressed in tumors with pathological features recognized as indicators of poor prognosis such as desmoplasia, perineural invasion and infiltrative growth pattern ( 17 ). Similarly, miR-205 was also demonstrated to be upregulated in ovarian cancer tissues compared with normal tissues ( 22 ), and enhanced the ability of cell invasion and migration by targeting vascular endothelial growth factor and Zinc Finger E-box binding homeobox 1 ( 23 , 24 ).…”