Micro<scp>RNA</scp>‐137 and micro<scp>RNA</scp>‐195* inhibit vasculogenesis in brain arteriovenous malformations

Huang, Jun; Song, Jianping; Qu, Meijie; Wang, Yang; An, Qingzhu; Song, Yaying; Yan, Wei; Wang, Bingshun; Wang, Xiaojin; Zhang, Song; Chen, Xi; Zhao, Bing; Liu, Peixi; Xu, Tongpeng; Zhang, Zhijun; Greenberg, David A.; Wang, Yongting; Gao, Pingjin; Zhu, Wei; Yang, Guo‐Yuan

doi:10.1002/ana.25015

Cited by 36 publications

(40 citation statements)

References 38 publications

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“…41,42 Previous studies demonstrated that NBP could promote angiogenesis. 38,39 The incidence of ischemic stroke and the infarct volume were decreased with NBP pretreatment in stroke-prone renovascular hypertensive rats. 22,43 In addition, NBP treatment within 24 hours after ischemic stroke rescued brain tissue by enhancing angiogenesis or opening collateral vessels, which was associated with the upregulation of VEGF and HIF-1α expression, in the stroke-prone renovascular hypertensive rats.…”

Section: Discussionmentioning

confidence: 99%

“…A tube formation assay was carried out as previously described. 39 Matrigel (BD Biosciences, Franklin Lakes, NJ) was added into a 96well plate and incubated at 37°C for 30 minutes to allow solidification. Then, 2 × 10 4 HUVECs suspended in 30% CM of the four astrocyte groups and 70% endothelial cell medium were seeded into the coated plate in triplicate.…”

Section: Cell Tube Formation Assaymentioning

confidence: 99%

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Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

Zhou

Wang

et al. 2019

CNS Neurosci Ther

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Summary Introduction Dl‐3‐N‐butylphthalide (NBP), a small molecule drug used clinically in the acute phase of ischemic stroke, has been shown to improve functional recovery and promote angiogenesis and collateral vessel circulation after experimental cerebral ischemia. However, the underlying molecular mechanism is unknown. Aims To explore the potential molecular mechanism of angiogenesis induced by NBP after cerebral ischemia. Results NBP treatment attenuated body weight loss, reduced brain infarct volume, and improved neurobehavioral outcomes during focal ischemia compared to the control rats ( P < 0.05). NBP increased the number of CD31 + microvessels, the number of CD31 + /BrdU + proliferating endothelial cells, and the functional vascular density ( P < 0.05). Further study demonstrated that NBP also promoted the expression of vascular endothelial growth factor and angiopoietin‐1 ( P < 0.05), which was accompanied by upregulated sonic hedgehog expression in astrocytes in vivo and in vitro. Conclusion NBP treatment promoted the expression of vascular endothelial growth factor and angiopoietin‐1, induced angiogenesis, and improved neurobehavioral recovery. These effects were associated with increased sonic hedgehog expression after NBP treatment. Our results broadened the clinical application of NBP to include the later phase of ischemia.

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Section: Discussionmentioning

confidence: 99%

Section: Cell Tube Formation Assaymentioning

confidence: 99%

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

Zhou

Wang

et al. 2019

CNS Neurosci Ther

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“…Intracranial aneurysm tissues were obtained from the brain tissues of 3 IA patients during microsurgery. The modified isolation protocol was based on a previously described study ( Bygglin et al, 2011 ; Huang et al, 2017 ). After clipping, the samples were immediately collected into DMEM supplemented with 5% penicillin/streptomycin.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Myosin Light Chain Kinase on the Occurrence and Development of Intracranial Aneurysm

Song

Liu

et al. 2018

Front. Cell. Neurosci.

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Myosin light chain kinase is a key enzyme in smooth muscle cell contraction. However, whether myosin light chain kinase plays a role in the occurrence or development of intracranial aneurysms is not clear. The present study explored the function of myosin light chain kinase in human intracranial aneurysm tissues. Five aneurysm samples and five control samples were collected, and smooth muscle cells (SMCs) were dissociated and cultured. A label-free proteomic analysis was performed to screen the differentially expressed proteins between aneurysm and control samples. The expression and function of myosin light chain kinase in aneurysms were examined. We found that 180 proteins were differentially expressed between the aneurysm and control samples, among which 88 were increased and 92 (including myosin light chain kinase) were decreased in aneurysms compared to control tissues. In a model of the inflammatory environment, contractility was weakened and apoptosis was increased in aneurysm SMCs compared to human brain SMCs (p < 0.05). The knock down of myosin light chain kinase in human brain SMCs caused effects similar to those observed in aneurysm SMCs. These results indicated that myosin light chain kinase plays an important role in maintaining smooth muscle contractility, cell survival and inflammation tolerance.

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“…The present study predicted the interaction of circRNAs and miRNAs. Among them, miR-137 targeted by circRNA_100933, has been reported to act as tumor suppressor in various tumors [ 41 , 42 ]. Previous study demonstrated that miR-216a-3p, one of the target miRNAs of circRNA_104310, inhibited gastric and colorectal cancer cell proliferation and migration [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Circular RNA profile of infantile hemangioma by microarray analysis

et al. 2017

PLoS ONE

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BackgroundCircular RNAs (circRNAs) are a recently identified class of noncoding RNAs that participate in several physiological processes. However, the expression of circRNAs in infantile hemangioma (IH) remains unknown.MethodsThe profile of circRNAs was assessed by microarray in four pairs of IH and adjacent skin tissues. The expression of circRNAs was validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, circRNA-microRNAs (miRNA)-mRNA networks were constructed using bioinformatics tools.Results234 up- and 374 down- regulated circRNAs were identified in IH by microarray. Among them, the expression of two up-regulated circRNAs (hsa_circRNA_100933 and hsa_circRNA_100709) and one down-regulated circRNA (hsa_circRNA_104310) was confirmed by qRT-PCR. In addition, 3,019 miRNA response elements (MREs) of circRNAs were predicted, and two circRNA-miRNA-mRNA networks were constructed, including 100 and 94 target genes of hsa_circRNA_100933 and hsa_circRNA_104310, respectively. GO and pathway analysis showed that both networks participated in angiogenesis and vascular development-related biological processes.ConclusionsThis is the first study to reveal the profiling of circRNAs in IH and pave the way for further characterization of the role of circRNAs in the pathogenesis of IH.

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MicroRNA‐137 and microRNA‐195* inhibit vasculogenesis in brain arteriovenous malformations

Abstract: Our results indicate that miR-137 and miR-195* act as vasculogenic suppressors in AVMs by altering phenotypic properties of AVMSMCs, and that the absence of miR-137 and miR-195* expression leads to abnormal vasculogenesis. Ann Neurol 2017;82:371-384.

Cited by 36 publications

References 38 publications

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

The Effect of Myosin Light Chain Kinase on the Occurrence and Development of Intracranial Aneurysm

Circular RNA profile of infantile hemangioma by microarray analysis

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