Micro-scaled topographies direct differentiation of human epidermal stem cells

Zijl, Sebastiaan; Vasilevich, Aliaksei; Viswanathan, Priyalakshmi; Helling, Ayelen Luna; Beijer, Nick R. M.; Walko, Gernot; Chiappini, Ciro; Boer, Jan de; Watt, Fiona M.

doi:10.1016/j.actbio.2018.12.003

Cited by 25 publications

(25 citation statements)

References 33 publications

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“…Consistently, topographies that prevent cell spreading or restrict surface coverage promote differentiation through a contractility-dependent mechanism (Zijl et al 2019). Although the precise mechanisms are unclear, these findings suggest that the 3-dimensional organization and topological features of the ECM play instructional roles in mediating epidermal stem cell fate decisions.…”

Section: Matrix Rigidity and Topologymentioning

confidence: 81%

Mechanical Forces in the Skin: Roles in Tissue Architecture, Stability, and Function

Biggs

Kim

Miroshnikova

et al. 2020

Journal of Investigative Dermatology

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Tissue shape emerges from the collective mechanical properties and behavior of individual cells and the ways by which they integrate into the surrounding tissue. Tissue architecture and its dynamic changes subsequently feed back to guide cell behavior. The skin is a dynamic, self-renewing barrier that is subjected to large-scale extrinsic mechanical forces throughout its lifetime. The ability to withstand this constant mechanical stress without compromising its integrity as a barrier requires compartment-specific structural specialization, and capability to sense and adapt to mechanical cues. This review discusses the unique mechanical properties of the skin and the importance of signals that arise from mechanical communication between cells and their environment. Adams JC, Watt FM. Fibronectin inhibits the terminal differentiation of human keratinocytes. Nature. 1989;340(6231):307-9 Adams JC, Watt FM. Changes in keratinocyte adhesion during terminal differentiation: Reduction in fibronectin binding precedes α5β1 integrin loss from the cell surface. Cell. 1990;63(2):425-35 Aw WY, Devenport D. Planar cell polarity: global inputs establishing cellular asymmetry.

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Section: Matrix Rigidity and Topologymentioning

confidence: 81%

Mechanical Forces in the Skin: Roles in Tissue Architecture, Stability, and Function

Biggs

Kim

Miroshnikova

et al. 2020

Journal of Investigative Dermatology

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“… 455 Several predictors have been identified that are able to reveal the effect of surface topography on cell morphology, 144 Oct4 expression in human induced PSCs, 564 osteogenic differentiation, 566 and epidermal stem cell differentiation. 568 …”

Section: Computational Tools For Materiobiology and Biomaterials Discoverymentioning

confidence: 99%

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

et al. 2021

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The complex interaction of cells with biomaterials (i.e., materiobiology) plays an increasingly pivotal role in the development of novel implants, biomedical devices, and tissue engineering scaffolds to treat diseases, aid in the restoration of bodily functions, construct healthy tissues, or regenerate diseased ones. However, the conventional approaches are incapable of screening the huge amount of potential material parameter combinations to identify the optimal cell responses and involve a combination of serendipity and many series of trial-and-error experiments. For advanced tissue engineering and regenerative medicine, highly efficient and complex bioanalysis platforms are expected to explore the complex interaction of cells with biomaterials using combinatorial approaches that offer desired complex microenvironments during healing, development, and homeostasis. In this review, we first introduce materiobiology and its high-throughput screening (HTS). Then we present an in-depth of the recent progress of 2D/3D HTS platforms (i.e., gradient and microarray) in the principle, preparation, screening for materiobiology, and combination with other advanced technologies. The Compendium for Biomaterial Transcriptomics and high content imaging, computational simulations, and their translation toward commercial and clinical uses are highlighted. In the final section, current challenges and future perspectives are discussed. High-throughput experimentation within the field of materiobiology enables the elucidation of the relationships between biomaterial properties and biological behavior and thereby serves as a potential tool for accelerating the development of high-performance biomaterials.

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“…In conjunction with computational modeling, this platform was able to predict topographical features conducive to maintaining PSC pluripotency, thus demonstrating great promise for exploring how to use tissue organization patterning to control cell fate. Application of this platform for probing keratinocyte differentiation revealed that differentiation is linked to changes in cell morphology, which is influenced by substrate topography, and mediated by Rho kinase activity [ 226 ].…”

Section: Opportunities To Exploit Mechanical Cues For Improving Direcmentioning

confidence: 99%

Current strategies and opportunities to manufacture cells for modeling human lungs

Varma

Soleas

Waddell

et al. 2020

Advanced Drug Delivery Reviews

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Chronic lung diseases remain major healthcare burdens, for which the only curative treatment is lung transplantation. In vitro human models are promising platforms for identifying and testing novel compounds to potentially decrease this burden. Directed differentiation of pluripotent stem cells is an important strategy to generate lung cells to create such models. Current lung directed differentiation protocols are limited as they do not 1) recapitulate the diversity of respiratory epithelium, 2) generate consistent or sufficient cell numbers for drug discovery platforms, and 3) establish the histologic tissue-level organization critical for modeling lung function. In this review, we describe how lung development has formed the basis for directed differentiation protocols, and discuss the utility of available protocols for lung epithelial cell generation and drug development. We further highlight tissue engineering strategies for manipulating biophysical signals during directed differentiation such that future protocols can recapitulate both chemical and physical cues present during lung development.

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Micro-scaled topographies direct differentiation of human epidermal stem cells

Abstract: Graphical abstract

Cited by 25 publications

References 33 publications

Mechanical Forces in the Skin: Roles in Tissue Architecture, Stability, and Function

Mechanical Forces in the Skin: Roles in Tissue Architecture, Stability, and Function

High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology

Current strategies and opportunities to manufacture cells for modeling human lungs

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