Micro RNA differential expression profile in canine mammary gland tumor by next generation sequencing

NGS-identified miRNAs in Canine Mammary Gland Tumors Show Unexpected Expression Alterations in qPCR Analysis

“…The samples targeted in this analysis were obtained from four different histological subtypes. The non-metastatic tissue comprised nine samples from our previous study (9); specifically, normal mammary gland (n=3; Sample Nos. : MG1-3), and adenoma (n=3; Aden1-3), and adenocarcinoma (n=3; AdCa1-3).…”

“…Sample collection. The canine mammary gland samples targeted for real-time PCR and NGS analyses in this study were from the same pool of tissue samples described in our previous study (9), and relevant information on these samples covering the breed and age of the relevant dog, and the diagnosis for the relevant case is presented in Table I. Basically, this pool consisted of normal tissue (n=7) and clinical (n=80) samples from female dogs.…”

“…This analysis included target miRNAs meeting the selection criterion for NGS results described above (normalized mean expression value>50 and FDR-p<0.05). Of these eight miRNAs, two (cfa-miR-133a-3p and cfa-miR-133c-3p) had already undergone the relevant analysis in our previous study (9). The other six target miRNAs (cfa-miR-187-3p, cfa-miR-202-5p, cfa-miR-424-5p, cfa-miR-450a-5p, cfa-miR-450b-5p, and cfa-miR-542-3p) were included in this analysis.…”

“…In our previous study, we used NGS followed by largescale qPCR to investigate miRNAs differentially expressed in canine MGTs relative to normal mammary gland tissue (9). Furthermore, differences in microRNA expression between non-metastatic and metastatic canine MGTs have already been reported in a microarray-based study (10), suggesting their expression profiles differ between at least some histological subtypes and indicating their potential as histological-subtype-specific biomarkers.…”

“…*The record for neutering status was the status before the dog subjected to the tumor removal. This table was referred from Micro RNA differential expression profile in canine mammary gland tumor by Next Generation Sequencing(9).…”