Micro Propagation and Organogenesis of Peperomia obtusifolia

El-Naggar, Hany M.; Osman, Amira R.

doi:10.3923/ajcs.2014.58.66

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…Means followed by the same letters in each column are not significantly different (P = 0.05) using Duncan's multiple range test 1.0 mg/l in combination with 0.7 mg/l NAA responded highest shoot regeneration (89 %) with highest number of shoots (25.57) and shoot length (3.87) on leaf derived callus (Table 3). Similar results have also been observed in leaf explants of in Peperomia obtusifolia (Naggar and Osman 2014) and Ziziphora tenuior (Dakah et al 2014). Likewise, internodal explants cultured on MS medium supplemented with KN (1.0 mg/l) and NAA (0.7 mg/l) showed higher regeneration frequency (85 %) and highest number (16.42) of shoots and shoot length (2.94).…”

Section: Effect Of Auxins On Shoot Regenerationsupporting

confidence: 82%

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

Thaniarasu

Kumar

Rao

2016

Physiol Mol Biol Plants

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The present study describes the plant propagation via indirect organogenesis from in vitro derived leaf and internode explants of Plectranthus bourneae, an endemic plant to south India. Leaf and internodal explants successfully callused on Murashige and Skoog medium (MS) supplemented with different concentrations of auxins [2,4-D (2, 4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid) and PIC (Picloram); 0.1-2.0 mg/l] in combination with BA (6-benzyladenine) (0.5 mg/l). Maximum callus induction (98 %) was achieved from leaf explant followed by internodal explant (89 %) at 1.0 mg/l NAA, 0.5 mg/l BA. Leaf derived callus showed better shoot regeneration (29.71 shoots) on MS medium containing 1.0 mg/l KN (kinetin), 0.7 mg/l NAA, and 50 mg/l CH (casein hydrolysate) followed by internodal callus (19.71). A maximum of 19.14 roots/shoot was observed at 1.0 mg/l IBA. The rooted plantlets were successfully hardened and transferred to greenhouse condition with 80 % survival. This system could be utilized for large-scale multiplication of P. bourneae by tissue culture.

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Section: Effect Of Auxins On Shoot Regenerationsupporting

confidence: 82%

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

Thaniarasu

Kumar

Rao

2016

Physiol Mol Biol Plants

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“…The filament explants of RDF CG/E genotypes gave better results than DZ CG/E and EXO CF/E genotypes, while in ovule explants, EXO CF/E genotype was better than DZ CG/E and RDF CG/E genotypes. In other studies, scale explants derived from in vitro cultures showed greater ability to induce callus than petioles and leaves of Lilium leucanthum (Tang et al, 2010); bulb scale explants produced a high percentage of shoot regeneration compared to leaf segments (El-Naggar et al, 2012); basal part of shoots induced better results on shoot formation than the tip part of shoots ; basal scale segments stimulated better results in the highest culture establishment compared to tip scale segments (Rafiq et al, 2021). Furthermore, fruity pink cultivar was more responsive induction callus media than cultivars of sorbon and purple diamond (Kurniati et al, 2011); lily hybrids cv.…”

Section: Discussion Discussion Discussionmentioning

confidence: 74%

“…Application of IM-2, IM-4 and IPM-7 for filament and ovule explants for the adventitious shoot formation, was also comparable to other research results (Tables 1-5) using leaf and bulb scales in an in vitro culture of Lilium. Bulb scales of Lilium longiflorum cv Easter lily planted on MS medium fortified by 18.09 µM 2,4-D stimulated 70% shoot organogenesis with 14 shoots/explant (Kanchanapoom et al, 2011), Lilium 'Prato' explants regenerated on MS medium fortified by 8.87 µM N-6 benzyladenine (BA) and 2.69 µM NAA produced 6 shoots/explant with 5.1 cm shoot height (El-Naggar et al, 2012), leaves cultured on the MS medium with 5.37 µM NAA and 2.27 µM TDZ regenerated 5.05 shoots and 36.5 leaves after 14 weeks incubation (Saetiew and Umamanit, 2015), leaf explants of Lilium orientalis 'Starfigther' on MS culture medium with 2.27 µM TDZ and 45.24 µM 2,4-D produced 8.3 shoots/explant, 5.4 leaves/shoot (Youssef et al, 2019), bulb scales planted on MS medium added by 5.37 µM NAA and 4.44 µM BAP stimulated 100% growing percentage with 4.3 shoots/explant and 1.4 microbulbs of Lilium longiflorum , basal scale segments of 'Oriental' hybrid Lilium 'Ravena' planted on MS medium 2.69 µM NAA and 8.88 µM BAP produced maximum culture establishment up to 76.2%, 5.5 bublets/explant, 2.2 cm shoot length and 3.4 leaves (Rafiq et al, 2021). These above results gave evident that each explant had optimal regeneration results in a specific culture medium.…”

Section: Discussion Discussion Discussionmentioning

confidence: 99%

Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp.

WINARTO,

KARTIKANINGRUM,

RACHMAWATI

et al. 2024

Not Bot Horti Agrobo

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Lilies are important cut and pot flowers locally and globally; however, qualified planting materials from in vitro culture are still limited. The culture generally uses scales and leaves, while filaments and ovules are rarely utilized. Filaments and ovules of EXO CF/E, RDF CG/E, and DZ CG/E genotypes of Lilium sp. were explored for their potential to establish in vitro propagation protocol using different Chu N6 medium and their modifications. The 9.9 shoots/explant from 1.5 regenerative explants/replication of RDF CG/E filaments was proven on Chu N6 medium containing 6.79 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 2.27 µM thidiazuron (TDZ), and 90 g L-1 sucrose; 3.3 regenerative explants/replication and 8.4 shoots/explant of EXO CF/E ovules were determined on Chu N6 medium with 2.26 µM 2,4-D, 6.81 µM TDZ and 90 g L-1 sucrose; and slightly improved on Chu N6 macro elements, full vitamins of MS basal medium with 3.39 µM 2,4-D, 3.41 µM TDZ, 150 ml L-1 coconut water (CW) and 60 g L-1 sucrose. The shoots were proliferated on the established media. The shoots of EXO CF/E, RDF CG/E and DZ CG/E genotypes produced varied leaves/shoot, leaf length, leaf width, roots/shoot, root length and bulb diameter on Chu N6 medium hormone free with 1.5% AC, followed by hardening them in a glass house for one month. The hardened-plantlets were acclimatized in plastic pots containing charcoal husk and bamboo compost (1:1) with 39.8-63.6% survivability. The protocol has the potential to be applied to other genotypes or lilies by specific improvements at the acclimatization stage.

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“…Further study demonstrated that explants exposed to a medium containing 3.0 mg LG 1 zeatin achieved 50% shoot responses and about 4.2 cm average shoot length 33 . In addition to that, 5 mg LG 1 kinetin combined with 1 mg LG 1 NAA induced 71% shoot proliferation 34,35 .…”

Section: Discussionmentioning

confidence: 80%

Embryo and Ovary Culture of Important Legume Horse Gram, Macrotyloma uniflorum (Lam.) Verdc

Karthika¹,

Venkatacha²,

Gurusamy³

et al. 2022

Asian J. of Crop Science

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Background and Objective: Macrotyloma uniflorum is nutritious and a good source of bioactive substances with high biological processes. The high frequency and efficient in vitro regeneration protocol have developed first time by utilizing embryo and ovary explants in Horsegram, Macrotyloma uniflorum. Materials and Methods: This research was carried out from horse gram paiyur-2 variety seeds derived embryo and ovary explants used by MS medium supplemented with cytokinins and auxins. Results: The phytohormone 0.4 mg LG 1 zeatin induced a maximum of 12 and 9 shoots from an embryo and ovary explants, respectively. The maximum frequency of rooting with about 9 roots was procured from the shoots cultured from embryonic explants using 0.2 mg LG 1 NAA and 7 roots were propagated from shoots derived from ovary explants. Overall studies observed that the embryonic explants show a high percent frequency of shoot and root induction when compared to ovary culture and further plantlets rose using embryonic explants with shoot growth period than the ovary explants. Conclusion: In vitro legume regeneration utilizing embryo and ovary is efficient, dependable and has fewer problems. Embryo explants were used to induce high-frequency plant regeneration, indicating that the ovaries are useful in genetic transformation.

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Micro Propagation and Organogenesis of Peperomia obtusifolia

Cited by 4 publications

References 12 publications

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

Potential utilization of filaments and ovules as explant sources in in vitro propagation of Lilium sp.

Embryo and Ovary Culture of Important Legume Horse Gram, Macrotyloma uniflorum (Lam.) Verdc

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