“…In particular, several microfluidics systems which allow the chemical environment of eukaryotic cells to be altered while simultaneously measuring the expression of key reporter genes by fluorescence microscopy have been proposed (Jovic, Howell, & Takayama, 2009;Taylor et al, 2009). A number of commercial systems exist; however, they are often limited in terms of operability and, without solid expertize, cannot be used to perform combinatorial and/or time-dependent cell-based assays (Castel, Pitaval, Debily, & Gidrol, 2006;Evenou, Di Meglio, Ladoux, & Hersen, 2012;Wu et al, 2010). A number of commercial systems exist; however, they are often limited in terms of operability and, without solid expertize, cannot be used to perform combinatorial and/or time-dependent cell-based assays (Castel, Pitaval, Debily, & Gidrol, 2006;Evenou, Di Meglio, Ladoux, & Hersen, 2012;Wu et al, 2010).…”