Application of Au cage/Ru(bpy)32+ nanostructures for the electrochemiluminescence detection of K562 cancer cells based on aptamer

Ge, Lei; Su, Min; Gao, Chaomin; Tao, Xutang; Ge, Shenguang

doi:10.1016/j.snb.2015.03.020

Cited by 32 publications

(7 citation statements)

References 33 publications

(26 reference statements)

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“…β-Cyclodextrin-AuNPs were used to load Ru(bpy) 3 2+ for the development of a "signal-on" switch ECL biosensor for the detection of MEAR cells [235]. Ge et al reported an ECL method for the detection of K563 cancer cells based on Au nanocages/Ru(bpy) 3 2+ molecules [236]. Hollow Au nanocages with porous structure and high surface area not only possessed more active sites to load a large amount of Ru(bpy) 3 2+ molecules, but also accelerate the electron communication.…”

Section: Electrochemiluminescence Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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The accurate analysis of circulating tumor cells (CTCs) holds great promise in early diagnosis and prognosis of cancers. However, the extremely low abundance of CTCs in peripheral blood samples limits the practical utility of the traditional methods for CTCs detection. Thus, novel and powerful strategies have been proposed for sensitive detection of CTCs. In particular, nanomaterials with exceptional physical and chemical properties have been used to fabricate cytosensors for amplifying the signal and enhancing the sensitivity. In this review, we summarize the recent development of nanomaterials-based optical and electrochemical analytical techniques for CTCs detection, including fluorescence, colorimetry, surface-enhanced Raman scattering, chemiluminescence, electrochemistry, electrochemiluminescence, photoelectrochemistry and so on.

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Section: Electrochemiluminescence Cytosensorsmentioning

confidence: 99%

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

2021

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“…On the other hand, PtNP-dotted CNTs were implemented on the electrode to immobilize the aptamer, improve the electronic transmission, and provide a large surface area. The K562 cancer cells were detected through the sandwich-type assay with a sensitivity of 500 cells/ml [175]. Several recent studies have been conducted on ECL cytosensors by taking advantage of AuNPs to carry cell recognizer or cell-specific receptors with high electron transfer capability [176, 177].…”

Section: The Functions Of Mnps In Various Biosensor Typesmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

295

119

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The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

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“…Electrochemiluminescence (ECL) has attracted wide attention and has been applied in multiple domains including biodetecting and biosensing technology. − However, finding suitable and stable luminescent materials is a major research problem in the construction of ECL sensors . Among numerous illuminants, Ru(bpy) 3 2+ is a prevalent luminophore because of its admirable ECL efficiency and favorable stability in aqueous solution and nonaqueous solvents .…”

Section: Introductionmentioning

confidence: 99%

“…6−11 However, finding suitable and stable luminescent materials is a major research problem in the construction of ECL sensors. 12 Among numerous illuminants, Ru(bpy) 3 2+ is a prevalent luminophore because of its admirable ECL efficiency and favorable stability in aqueous solution and nonaqueous solvents. 13 These properties generate preponderances such as demanding minimal amounts of costly or poisonous reagents and simplified experimental design considerations especially in the solid-phase immobilized applications.…”

Section: ■ Introductionmentioning

confidence: 99%

Quenching Electrochemiluminescence Immunosensor Based on Resonance Energy Transfer between Ruthenium (II) Complex Incorporated in the UiO-67 Metal–Organic Framework and Gold Nanoparticles for Insulin Detection

Zhao

Wang

et al. 2018

ACS Appl. Mater. Interfaces

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This work describes a sandwich-type electrochemiluminescence (ECL) strategy for insulin detection by using Ru(bpy) as the luminophore which was encapsulated in the UiO-67 metal-organic framework (UiO-67/Ru(bpy)). Because UiO-67 possesses the characteristics of large specific surface area and porosity, more Ru(bpy) could be loaded onto its surface and holes, thus greatly improving the ECL efficiency. Furthermore, the ECL resonance energy transfer (ECL-RET) could occur between UiO-67/Ru(bpy) (ECL donor) and Au@SiO nanoparticles (ECL acceptor), resulting in a conspicuously decreased ECL response. The ECL spectrum of UiO-67/Ru(bpy) which exhibited strong ECL intensity has suitable spectral overlap with the absorption spectrum of Au@SiO, which further proved the occurrence of the ECL-RET action. The ECL intensity decreased with the increase of the concentration of insulin. In addition, the sandwich-type ECL immunosensor was applied to insulin detection, and the ECL decrease efficiency was found to be logarithmically related to the concentration of the insulin antigen in the range of 0.0025 to 50 ng mL with the limit of detection of 0.001 ng mL. Meanwhile, this work provides an important reference for the application of metal-organic frameworks in the ECL and ECL-RET study and also exhibits potential capability in the detection of other hormones.

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Application of Au cage/Ru(bpy)32+ nanostructures for the electrochemiluminescence detection of K562 cancer cells based on aptamer

Cited by 32 publications

References 33 publications

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Recent Development of Nanomaterials-Based Cytosensors for the Detection of Circulating Tumor Cells

Noble metal nanoparticles in biosensors: recent studies and applications

Quenching Electrochemiluminescence Immunosensor Based on Resonance Energy Transfer between Ruthenium (II) Complex Incorporated in the UiO-67 Metal–Organic Framework and Gold Nanoparticles for Insulin Detection

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