Micro Open-Sandwich ELISA to Rapidly Evaluate Thyroid Hormone Concentration from Serum Samples

Islam, Kamrun Nahar; Ihara, Masaki; Dong, Jinhua; Kasagi, Noriyuki; Mori, Toshihiro; Ueda, Hiroshi

doi:10.4155/bio.10.125

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…With a similar system setup, Islam et al succeeded in the detection of a thyroid hormone thyroxine (3,3′,5,5′‐tetraiodothyronine; T4) with high sensitivity . T4 is a major thyroid hormone and often used for evaluation of thyroid function.…”

Section: Open Sandwich On Microfluidic Devicesmentioning

confidence: 99%

“…With a similar system setup, Islam et al succeeded in the detection of a thyroid hormone thyroxine (3,3 0 ,5,5 0 -tetraiodothyronine; T4) with high sensitivity. 58 T4 is a major thyroid hormone and often used for evaluation of thyroid function. Stimulation of the thyroid gland by thyroid-stimulating hormone causes the release of T4 into the bloodstream, which plays a major role in the regulation of metabolic processes.…”

Section: Open-sandwich Microelisa To Detect Thyroid Hormonementioning

confidence: 99%

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ELISA‐type assays of trace biomarkers using microfluidic methods

Dong

Ueda

2017

WIREs Nanomed Nanobiotechnol

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Recently, great progress has been achieved for analytical technologies for biological substances. Traditionally, detection methods for analytes mainly rely on large instrumental analyses. These methods require costly equipment, skilled operators and long measurement time despite their generally low sensitivity. In contrast, immunoassays are becoming more and more popular for it is powerful, inexpensive, and convenient nature. Immunoassay has a range of applications, because it employs antibody, a protein produced by plasma cells in the acquired immune system to identify and neutralize diverse pathogens and other exogenous substances. However, the sensitivity of conventional immunoassays so far is limited by their reaction principles and detection methods. The microfluidics technology is the one that manipulates small volumes of fluid and flow, which has the potential to miniaturize many laboratory procedures. Immunoassays on microfluidic devices have been studied extensively and have gained significant attention owing to intrinsic advantages offered by the assay platforms. The techniques have allowed the miniaturization of conventional immunoassay and bring the advantages such as small volumes of samples and reagents as well as the decrease of contamination, which results in the decline of false-positive results. Ultimately, the combination of immunoassays with microfluidics affords a promising platform for multiple, sensitive, and automatic point-of-care diagnostics. Recent achievements on microfluidic devices and immunoassay detection systems including digital assay employing single molecule will be introduced in detail and the strategies for faster and more sensitive configurations in microfluidic immunosensors will be highlighted. WIREs Nanomed Nanobiotechnol 2017, 9:e1457. doi: 10.1002/wnan.1457 For further resources related to this article, please visit the WIREs website.

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Section: Open Sandwich On Microfluidic Devicesmentioning

confidence: 99%

Section: Open-sandwich Microelisa To Detect Thyroid Hormonementioning

confidence: 99%