Micro-NMR on CMOS for Biomolecular Sensing

Lei, Ka-Meng; Sun, Nan; Mak, Pui-In; Martins, Rui P.; Ham, Donhee

doi:10.1007/978-3-319-67723-1_5

Cited by 4 publications

(3 citation statements)

References 73 publications

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“…Once completed, the system can be used to re-supply water, food, and nutrients to the organisms or in the future even expose the organisms to contaminants under automation. Previously, DMF-NMR has been utilized for reaction monitoring, kinetics, and biomolecular sensing, ,, but this is the first attempt to sustain organisms in vivo inside an NMR. Furthermore, while DMF cell culturing is relatively common, we are aware of only a single previous report that describes the use of DMF to culture multi-cellular organisms .…”

Section: Resultsmentioning

confidence: 99%

Integrated Digital Microfluidics NMR Spectroscopy: A Key Step toward Automated In Vivo Metabolomics

et al. 2023

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Toxicity testing is currently undergoing a paradigm shift from examining apical end points such as death, to monitoring sub-lethal toxicity in vivo. In vivo nuclear magnetic resonance (NMR) spectroscopy is a key platform in this endeavor. A proof-of-principle study is presented which directly interfaces NMR with digital microfluidics (DMF). DMF is a “lab on a chip” method allowing for the movement, mixing, splitting, and dispensing of μL-sized droplets. The goal is for DMF to supply oxygenated water to keep the organisms alive while NMR detects metabolomic changes. Here, both vertical and horizontal NMR coil configurations are compared. While a horizontal configuration is ideal for DMF, NMR performance was found to be sub-par and instead, a vertical-optimized single-sided stripline showed most promise. In this configuration, three organisms were monitored in vivo using 1H-13C 2D NMR. Without support from DMF droplet exchange, the organisms quickly showed signs of anoxic stress; however, with droplet exchange, this was completely suppressed. The results demonstrate that DMF can be used to maintain living organisms and holds potential for automated exposures in future. However, due to numerous limitations of vertically orientated DMF, along with space limitations in standard bore NMR spectrometers, we recommend future development be performed using a horizontal (MRI style) magnet which would eliminate practically all the drawbacks identified here.

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Section: Resultsmentioning

confidence: 99%

Integrated Digital Microfluidics NMR Spectroscopy: A Key Step toward Automated In Vivo Metabolomics

et al. 2023

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“…There are many books and reviews highlighting the recent advances of μNMR systems as well as the μNMR for real-life applications, and we list some of them for readers’ information. 130 − 133 .…”

Section: Nmr Platformsmentioning

confidence: 99%

“…In recent years, there have been many advances in miniaturizing the NMR platforms such as assembling electronics into integrated-circuit chips, implementing smaller or planar NMR coils and compact permanent magnets and mounting microfluidic channels. − These low-cost micro-NMR (μNMR) platforms have demonstrated the portability, robustness, versability, and even higher sensitivity than conventional systems. There are many books and reviews highlighting the recent advances of μNMR systems as well as the μNMR for real-life applications, and we list some of them for readers’ information. − .…”

Section: Nmr Platformsmentioning

confidence: 99%

Magnetic-Nanosensor-Based Virus and Pathogen Detection Strategies before and during COVID-19

Saha

et al. 2020

ACS Appl. Nano Mater.

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The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease 2019 (COVID-19), is a threat to the global healthcare system and economic security. As of July 2020, no specific drugs or vaccines are yet available for COVID-19; a fast and accurate diagnosis for SARS-CoV-2 is essential in slowing the spread of COVID-19 and for efficient implementation of control and containment strategies. Magnetic nanosensing is an emerging topic representing the frontiers of current biosensing and magnetic areas. The past decade has seen rapid growth in applying magnetic tools for biological and biomedical applications. Recent advances in magnetic nanomaterials and nanotechnologies have transformed current diagnostic methods to nanoscale and pushed the detection limit to early-stage disease diagnosis. Herein, this review covers the literature of magnetic nanosensors for virus and pathogen detection before COVID-19. We review popular magnetic nanosensing techniques including magnetoresistance, magnetic particle spectroscopy, and nuclear magnetic resonance. Magnetic point-of-care diagnostic kits are also reviewed aiming at developing plug-and-play diagnostics to manage the SARS-CoV-2 outbreak as well as preventing future epidemics. In addition, other platforms that use magnetic nanomaterials as auxiliary tools for enhanced pathogen and virus detection are also covered. The goal of this review is to inform the researchers of diagnostic and surveillance platforms for SARS-CoV-2 and their performances.

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µ‐NMR at the point of care testing

et al. 2020

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NMR shows strong analytical capability for obtaining molecular information on materials and is used in a variety of fields. Micro‐NMR (µNMR) is mainly based on low‐field NMR (LF‐NMR), which makes NMR detection portable and inexpensive. Point‐of‐care testing (POCT) has gradually become an area of major concern, and scientists have made much progress in applying µNMR systems for POCT. To the best of our knowledge, this is the first review of the latest development in miniaturization of µNMR systems. Then, we discuss cutting‐edge µNMR‐based applications in POCT and the outlook for future developments.

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Micro-NMR on CMOS for Biomolecular Sensing

Cited by 4 publications

References 73 publications

Integrated Digital Microfluidics NMR Spectroscopy: A Key Step toward Automated In Vivo Metabolomics

Integrated Digital Microfluidics NMR Spectroscopy: A Key Step toward Automated In Vivo Metabolomics

Magnetic-Nanosensor-Based Virus and Pathogen Detection Strategies before and during COVID-19

µ‐NMR at the point of care testing

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