µ‐NMR at the point of care testing

Jie, Guo; Jiang, Di; Feng, Shilun; Ren, Chunhui; Guo, Jinhong

doi:10.1002/elps.201900329

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…Furthermore, biomarkers of infection, prognosis, and those to guide and monitor therapy, could be different, which suggests the potential need for this type of analytical techniques after the biomarker discovery phase. With that in mind, there have been advances in the miniaturization of NMR equipment towards their application in point-of-care devices [35,36]. There are diverse studies of metabolomics of plasma, serum, or urine, that enabled to build good models to predict infection in relation to healthy volunteers, and infection among non-infected critically ill patients and patients presenting confound pathophysiological processes (e.g., mechanical ventilated patients, cancer patients with neutropenia, etc.,) (Table 1).…”

Section: Biomarkersmentioning

confidence: 99%

Infection Biomarkers Based on Metabolomics

et al. 2022

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Current infection biomarkers are highly limited since they have low capability to predict infection in the presence of confounding processes such as in non-infectious inflammatory processes, low capability to predict disease outcomes and have limited applications to guide and evaluate therapeutic regimes. Therefore, it is critical to discover and develop new and effective clinical infection biomarkers, especially applicable in patients at risk of developing severe illness and critically ill patients. Ideal biomarkers would effectively help physicians with better patient management, leading to a decrease of severe outcomes, personalize therapies, minimize antibiotics overuse and hospitalization time, and significantly improve patient survival. Metabolomics, by providing a direct insight into the functional metabolic outcome of an organism, presents a highly appealing strategy to discover these biomarkers. The present work reviews the desired main characteristics of infection biomarkers, the main metabolomics strategies to discover these biomarkers and the next steps for developing the area towards effective clinical biomarkers.

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Section: Biomarkersmentioning

confidence: 99%

Infection Biomarkers Based on Metabolomics

et al. 2022

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“…Other NATs are under development and a few are currently in use. These include CRISPR, isothermal amplification, and next-generation sequencing (NGS) (Broughton et al 2020 ; Guo et al 2020 ; Nat Biotechnol 2020 ; Weissleder et al 2020 ; Yoshikawa et al 2020 ). CRISPR-Cas12- and CRISPR-Cas13-based assays simultaneously perform reverse transcription and isothermal amplification from RNA extracted from nasopharyngeal or oropharyngeal swabs; then, it goes through Cas12 or Cas13 detection of coronavirus sequences with no cross reactivity from related coronavirus strains (Broughton et al 2020 ).…”

Section: Development Of Diagnosticsmentioning

confidence: 99%

A comprehensive review of COVID-19 biology, diagnostics, therapeutics, and disease impacting the central nervous system

et al. 2021

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Graphical abstract The ongoing COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a highly transmissible disease. SARS-CoV-2 is estimated to have infected over 153 million people and to have caused over 3.2 million global deaths since its emergence in December 2019. SARS-CoV-2 is the seventh coronavirus known to infect humans, and like other coronaviruses, SARS-CoV-2 infection is characterized by a variety of symptoms including general flu-like symptoms such as a fever, sore throat, fatigue, and shortness of breath. Severe cases often display signs of pneumonia, lymphopenia, acute kidney injury, cardiac injury, cytokine storms, lung damage, acute respiratory distress syndrome (ARDS), multiple organ failure, sepsis, and death. There is evidence that around 30% of COVID-19 cases have central nervous system (CNS) or peripheral nervous system (PNS) symptoms along with or in the absence of the previously mentioned symptoms. In cases of CNS/PNS impairments, patients display dizziness, ataxia, seizure, nerve pain, and loss of taste and/or smell. This review highlights the neurological implications of SARS-CoV-2 and provides a comprehensive summary of the research done on SARS-CoV-2 pathology, diagnosis, therapeutics, and vaccines up to May 5.

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“…181 ) Planar microcoils are easier to fabricate down to nL volumes and find applications in on-chip NMR devices. 182 Recently, Sahin Solmaz et al have demonstrated a silicon-chip-based device for DNP NMR of aqueous solutions of the TEMPO radical at room temperature. 183 A sample volume of about 1 nL is contained in a capillary that is placed over the double NMR/EPR microcoils.…”

Section: Instrumental Challenges and Outlook Formentioning

confidence: 99%

Challenges and Advances in the Application of Dynamic Nuclear Polarization to Liquid-State NMR Spectroscopy

Abhyankar

Szalai

2021

J. Phys. Chem. B

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Nuclear magnetic resonance (NMR) spectroscopy is a powerful method to study the molecular structure and dynamics of materials. The inherently low sensitivity of NMR spectroscopy is a consequence of low spin polarization. Hyperpolarization of a spin ensemble is defined as a population difference between spin states that far exceeds what is expected from the Boltzmann distribution for a given temperature. Dynamic nuclear polarization (DNP) can overcome the relatively low sensitivity of NMR spectroscopy by using a paramagnetic matrix to hyperpolarize a nuclear spin ensemble. Application of DNP to NMR can result in sensitivity gains of up to four orders of magnitude compared to NMR without DNP. Although DNP NMR is now more routinely utilized for solid-state (ss) NMR spectroscopy, it has not been exploited to the same degree for liquid-state samples. This Review will consider challenges and advances in the application of DNP NMR to liquid-state samples. The Review is organized into four sections: (i) mechanisms of DNP NMR relevant to hyperpolarization of liquid samples; (ii) applications of liquid-state DNP NMR; (iii) available detection schemes for liquid-state samples; and (iv) instrumental challenges and outlook for liquid-state DNP NMR.

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µ‐NMR at the point of care testing

Cited by 8 publications

References 37 publications

Infection Biomarkers Based on Metabolomics

Infection Biomarkers Based on Metabolomics

A comprehensive review of COVID-19 biology, diagnostics, therapeutics, and disease impacting the central nervous system

Challenges and Advances in the Application of Dynamic Nuclear Polarization to Liquid-State NMR Spectroscopy

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