The first step in the synthesis of functionalized polyamides consisted in the introduction of alkyne side groups in several types of linear polyamides by interfacial step-growth polymerization of hexane-1,6-diamine or 4,9-dioxadodecane-1,12-diamine in combination with on one hand adipoyl or sebacoyl chloride and on the other hand an alkyne-containing building block, i.e. 2-methyl-2-propargylmalonic acid dichloride. Both homo-and copolyamides were synthesized, creating a large range of alkyne-containing polyamides with degrees of functionalization ranging between 5 and 100 %. Subsequently, these polyamide chains have been modified with two types of linking chemistries, respectively with azides through the copper(I)-catalyzed Huisgen 1,3-dipolar cycloaddition or with thiols through the thiol-yne addition reaction.