Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of <i>Vanda tessellata</i> (Roxb.) Hook. ex G. Don

Manokari, M.; Rasangam, Latha; Selvam, Priyadharshini; Shekhawat, Mahipal S.

doi:10.1002/jemt.23630

Cited by 8 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acclimatization to ex vitro conditions is a critical step in any micropropagation protocol as plants produced in vitro often have dysfunctional stomata and a thin cuticle layer [12]. In the present study, all of the plantlets survived acclimatization to the glasshouse and mainatined high quality.…”

Section: Discussionmentioning

confidence: 59%

Establishment of an Efficient In Vitro Culture System in Dicentra × hybrida

Kulus

2020

The 1st International Electronic Conference on Plant Science

View full text Add to dashboard Cite

Dicentra × hybrida is a popular ornamental hybrid, cultivated in parks and gardens worldwide. To date, there are no reports on establishing a plant tissue culture system in this species. This study aims to develop an efficient micropropagation protocol in D. × hybrida ‘Amore Rose’ for further reproduction and breeding. Shoots of in vivo-grown plants were dissected and washed with running tap water. Next, shoot segments were bathed in tap water with a drop of detergent for 30 min; then, immersed in 70% (v/v) ethanol for 1 min. Following the initial disinfection, explants were surface sterilized with 0.4–0.8% (v/v) sodium hypochlorite (NaOCl) for 20 min and, finally, washed thrice with sterile distilled water (20 min each). The rosette explants were inoculated polarly in the modified Murashige and Skoog medium devoid of plant growth regulators and cultured in a growth room with a 16 h photoperiod. After one month, contamination-free explants were transferred on the kinetin-supplemented medium. The disinfection efficiency reached 10–40%. All disinfected explants were capable of developing healthy shoots. The multiplication ratio, i.e., the number of secondary explants produced, reached up to 6.0. All developed shoots regenerated roots spontaneously. The obtained plantlets were successfully acclimatized to ex vitro conditions. The described here protocol can be recommended for the in vitro propagation of Dicentra × hybrida.

show abstract

Section: Discussionmentioning

confidence: 59%

Establishment of an Efficient In Vitro Culture System in Dicentra × hybrida

Kulus

2020

The 1st International Electronic Conference on Plant Science

View full text Add to dashboard Cite

show abstract

“…Abiotic stress responses of different gypsophyte were extensively studied at the phytochemical and molecular level. Macromorphological and complementary microscope‐based techniques are helpful in interpreting the response of plants to abiotic stress at the anatomical and ultrastructural level (Han et al, 2021; Khan & Khan, 2020; Kim, 2018; Mani, Rasangam, Selvam, & Shekhawat, 2021; Nazir et al, 2021; Nazish et al, 2019; Noor & Ahmad, 2021; Öztürk Çalı & Karavin, 2020; Sadia et al, 2020; Salem‐Fnayou, Bouamama, Ghorbel, & Mliki, 2011; Scocco, Mercati, Tardella, & Catorci, 2016). While plant adaptation to changing environmental conditions can be associated with the organization of leaves, presence of a protective outer layer of the epidermis and cuticle, and properties of the stomata (Salem‐Fnayou et al, 2011), few reports described the anatomical and ultrastructural changes that characterize gypsophytes.…”

Section: Introductionmentioning

confidence: 99%

Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

Kayabaş

2021

Microscopy Res & Technique

View full text Add to dashboard Cite

Alyssum nezaketiae is a local endemic species in gypsum habitats of Çankırı province in Turkey. In this study, I used field collected specimens of A. nezaketiae in a scanning electron microscopy examination of vegetative and reproductive structures of A. nezaketiae. The objective of the study was to investigate the micromorphological properties that define the species and that represent its adaptation(s) to the extreme environmental conditions posed by gypsum habitats. The epidermis had one to two rows at transverse sections of leaf and stem. Cortex was composed six to eight rows parenchyma cells at stem. A number of adaptations to xeric habitats were observed, including: dense silvery lepidote hairs on leaves and stems, small leaves, stomata on the lower surfaces of the leaves occurring in stomatal crypts, intense lignification in the central cylinder of the stems, and bundles of sclerenchyma in the pith region of stems. These can be interpreted as gypsophytic characteristics associated with physiological drought. Micromorphological properties of plant parts are discussed in relation to environmental conditions and possible constraint factors occurring in these gypsum habitats. Furthermore, these micromorphological studies contribute important taxonomic characters that are needed to help identify a species whose habitat is under threat from a variety of human activities.

show abstract

“…For this reason, the vein shapes represent the basis for studies in many disciplines. However, there are very limited numbers of studies about the vein anatomy and vein patterns in orchids, especially (Cameron and Dickison, 1998;Mani et al, 2021). This research comparatively investigates the vein shapes and structure of 11 genera among representative species included in the Orchidaceae family (Cephalanthera Rich., Coeloglossum Lindl., Dactylorhiza Neck.…”

Section: Introductionmentioning

confidence: 99%

Comparative vascular anatomies of some orchid species

Şeker

Şenel

Akbulut

2021

Anatolian Journal of Botany

View full text Add to dashboard Cite

In this study, we examined the vascular anatomy of leaves with different morphological features of 11 orchid species. Plant samples were collected from various localities in the Black Sea Region. Fresh leaves were dried and stocked, and their vascular structures were analyzed by clearing and staining. Significant differences were determined in the leaves of taxa in terms of characters such as total leaf perimeter and area, number of veins and nodes, total vein length, total vein area, average vein length, average vein width, average vein surface area, average vein volume, and average areolar area. According to the findings, the topological and morphometric features of the veining can reflect the systematic and phylogenetic relationships of orchids.

show abstract

Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don

Cited by 8 publications

References 52 publications

Establishment of an Efficient In Vitro Culture System in Dicentra × hybrida

Establishment of an Efficient In Vitro Culture System in Dicentra × hybrida

Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

Comparative vascular anatomies of some orchid species

Contact Info

Product

Resources

About