As a result of the detailed literature reviews, it has been determined that there are a limited number of studies on the stolon anatomy of orchids. In this study, Ophrys mammosa subsp. mammosa taxon by examining the stolon anatomy of the, it is aimed to contribute to the explanation of the tuber ontogeny of the genus. As material, microtome sections were taken from the embedding preparations with historesin kit in Ophrys mammosa subsp. mammosa stolons and stained with toluidin blue. From anatomical sections; It was determined that the vascular bundles in the middle part of the stolon are spaced and meristele distributed asymmetrically and the presence of statocyte cells in the lower part, the presence of a velamen in the outermost and several rows of exodermis under it, and an inner parenchymatic wide cortex. As a result; It has been emphasized that the anatomy of the orchid stolon is partially similar to the tuber that will develop in the future, and the importance of the findings in terms of tuber biology is discussed.