Micro liquid chromatography – diode array detection of hop bitter acids

Sandra, Pat; Steenbeke, G.; Ghys, Marleen; Schomburg, G.

doi:10.1002/jhrc.1240130721

Cited by 12 publications

(9 citation statements)

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“…Photodiode array (PDA) detection has been studied extensively in microcolumn LC research [143][144][145][146][147]. In practice it is, however, hardly ever applied for structural conformation detection.…”

Section: Uv Absorbance Detectionmentioning

confidence: 99%

“…Ten-times lower limits of detection were found with capillary LC -despite the small optical path length of the PDA flow cell. Another example is the work of Sandra et al [143], who applied PDA detection in capillary LC for the detection and identification of hop bitter acids from CO2-extracted hop samples. The recorded PDA UV spectra showed that two groups could be differentiated: a-acids (humulones) and /3-acids (lupulones).…”

Section: Uv Absorbance Detectionmentioning

confidence: 99%

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Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Uv Absorbance Detectionmentioning

confidence: 99%

Section: Uv Absorbance Detectionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…Figures 2 and 3 show some typical applications. For the analysis of hop bitter acids, however, the PMSCN phase proved to be more selective for the separation of the six bitter principles [6].…”

Section: Reversed Phase Polymer Coatingsmentioning

confidence: 99%

“…Very few data, however, have been published on the use of polymercoated stationary phases in micro-LC [6]. Polymer stationary phase coatings were pioneered by Schomburg and co-workers ( [7] and references cited therein).…”

Section: Introductionmentioning

confidence: 98%

Polymeric coatings in packed fused silica micro‐LC columns

Ghijs

Sandra

1991

J Microcolumn Separations

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Abstract. Different polymercoated stationary phases have been evaluated in micro-LC: Nucleosil-100-5-Cl-PMSClS (C,,-substituted polymethylsiloxane, C1-deactivated with trimethylsilylenolate) and Nucleosil-100-5-C1-PMSCN (cyanopropyl-substituted polymethylsiloxane, Cl-deactivated) in the reversed phase mode and Nucleosil-100-5-PEG (polyethylene glycol-coated) in the normal phase mode.Fused silica tubing of 320-pm i.d. was slurry packed with suspensions optimized for each packing material. The micropacked columns were studied for efficiency and selectivity. The silanol activities of the polymer coated phases were compared to monomeric phases for the reversed phase mode and to silica for the normal phase mode.

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“…HPLC with UV detection is routinely used to analyze bitter acids [2][3][4][5][6][7][8][9]. The sensitivity and selectivity of UV are insufficient for direct identification of hop acids, especially for the identification of minor hop acids in complex mixtures.…”

mentioning

confidence: 99%

Direct characterization of bitter acids in a crude hop extract by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

Zhang

Liang

Xiao

et al. 2004

J. Am. Soc. Mass Spectrom.

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The applicability of on-line coupling of reversed-phase high-performance liquid chromatography to atmospheric pressure ionization tandem mass spectrometry for the separation and characterization of hop acids mixture from the crude extract of Humulus lupulus was investigated. The solvent system consisting of acetonitrile-aqueous formic acid was used to give proper separation of the six main hop bitter acids within 30 min. Further structural information about the components was acquired by collision-induced dissociation (CID). On the basis of analyses of the fragmentation patterns of the major ␣-and ␤-bitter acids respectively, identification of the minor ones was performed using selected reaction monitoring (SRM) with a group of qualitatively relevant selected precursor-product ion transitions for each bitter acid in a single high performance liquid chromatography (HPLC) run. Using this technique, six minor hop acids, including "adprelupulone" observed for the first time in natural resources, were detected along with the six major acids. This hyphenated techniques provides potency for rapid qualitative determination of analogs and homologs in

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Micro liquid chromatography – diode array detection of hop bitter acids

Cited by 12 publications

References 1 publication

Microcolumn liquid chromatography: instrumentation, detection and applications

Microcolumn liquid chromatography: instrumentation, detection and applications

Polymeric coatings in packed fused silica micro‐LC columns

Direct characterization of bitter acids in a crude hop extract by liquid chromatography-atmospheric pressure chemical ionization mass spectrometry

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