Micro-flow-injection analysis (μFIA) immunoassay of herbicide residue 2,6-dichlorobenzamide – towards automated at-line monitoring using modular microfluidics

Uthuppu, Basil; Heiskanen, Arto; Kofoed, Dan; Aamand, Jens; Jørgensen, Claus; Dufva, Martin

doi:10.1039/c4an01576b

Cited by 16 publications

(11 citation statements)

References 29 publications

(40 reference statements)

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“…[10][11][12][13] On-chip integration is superior to conventional FIA methods as it reduces analysis time, as well as sample and reagent consumption. These advantages raise the possibility of analytical applications in diverse elds such as environmental testing, [13][14][15][16] medical diagnosis, 8,16 food analysis, 17 and drug testing. 3,5,11 However, mFIA systems are prone to suffering from reduced analytical performance resulting from dead volumes in the injection module and tubing connections more than conventional FIA systems.…”

Section: Introductionmentioning

confidence: 99%

Development of an on-chip sample injection system with a 6-port valve incorporated in a microchip

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Development of an on-chip sample injection system with a 6-port valve incorporated in a microchip

et al. 2020

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“…Besides enhanced enzyme activity, temperature increase supports mass transfer and electron transfer, which may contribute to the observed offset. Further, temperature control in general may be convenient to reduce variations of the sensor response as described in literature [ 19 ]. Unsatisfying is an almost unchanged LOD of 5.4 ng/mL for the calibration curve at 30 °C compared to a LOD of 5.8 ng/mL without temperature control.…”

Section: Resultsmentioning

confidence: 99%

“…Further examples are a microfluidic immunosensor array for cytokine and C-reactive protein (CRP) determination [ 16 ], and a microfluidic immunosensor platform that allows multiplexed antibiotic detection [ 17 ]. Moreover, electrochemical immunosensor systems were used for on-line monitoring of bioreactor products [ 18 ] as well as for at-line monitoring of herbicide residues in water [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection

Metzner

Luckert

Lemuth

et al. 2018

Sensors

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The cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out.

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“…Another example involves an amperometric immunoassay for determination of residues of an organochloride pesticide (2,6-dichlorobenzamide) carried out in a microfluidic device. 65 The immunosorbent was covalently immobilized in one of the chip channels and the long-term stability required for at line monitoring of the pollutant in water supplies was achieved.…”

Section: Looking Forward: Research Perspectives On Flow Analysismentioning

confidence: 99%

“…64 Other applications discussed in the present review also exploited miniaturized systems. 51,61,65 Flow analysis has been pointed out as a powerful tool for green analytical chemistry. 66 Minimization of reagent consumption and waste generation is inherent to the process, but it is more evident in micro-flow analyzers, 19,51 as well as in some approaches such as merging zones, 7 SIA, 57 multicommutation, 11 and multipumping.…”

Section: Looking Forward: Research Perspectives On Flow Analysismentioning

confidence: 99%

Flow Analysis: Looking Back and Forward

Rocha¹

2018

J. Braz. Chem. Soc.

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Flow analysis changed significantly the way of performing chemical analysis by (i) mechanization of analytical procedures in a continuous flowing stream with minimal sample intercontamination; (ii) analytical measurements without attaining chemical equilibria, thus increasing the applicability of kinetic methods and allowing the exploitation of non-quantitative processes; (iii) exploitation of concentration gradients and (iv) use of unstable reagents or measurement of unstable products. Flow-based procedures are generally characterized by high sample throughput, improved precision, minimized waste generation, and often better selectivity. The performance of detection systems is usually improved because of the in-line sample conditioning and measurements under reproductive dynamic conditions and timing. However, after achieving several innovations, perspectives for further developments have been questioned mainly because of the recent decrease in the number of publications on flow analysis. The aim of this review is then to discuss the impact of flow-based methods on chemical analysis, emphasizing recent applications and developments, including miniaturization, bioanalysis, microextractions, green analytical chemistry and synergic hyphenation with other techniques and processes. The author's personal view about research perspectives in the field is also presented.

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Micro-flow-injection analysis (μFIA) immunoassay of herbicide residue 2,6-dichlorobenzamide – towards automated at-line monitoring using modular microfluidics

Abstract: A prototype microfluidic immunosensor for detecting 2,6-dichlorobenzamide showing potential for at-line monitoring of ground water.

Cited by 16 publications

References 29 publications

Development of an on-chip sample injection system with a 6-port valve incorporated in a microchip

Development of an on-chip sample injection system with a 6-port valve incorporated in a microchip

Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection

Flow Analysis: Looking Back and Forward

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