Micro-Electrophoresis of Protein on Filter-Paper*1

Flynn, F. V.; Mayo, Patricia

doi:10.1016/s0140-6736(51)93239-4

Cited by 268 publications

(31 citation statements)

References 7 publications

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“…Blood haemoglobin was estimated by the method of Sahli; total serum proteins by the copper-sulphate specific gravity method. The apparatus of Flynn and de Mayo (1951) was used for electrophoresis of the serum on filter paper, and the recording Photodensitometer of Joyce, Loebl and Co. for the tracing of the serum protein patterns.…”

Section: Pathological Changes In Rats Asmentioning

confidence: 99%

Pathological Changes in Rats as a Result of Treatment with Monocrotaline

Schoental¹,

Head²

1955

Br J Cancer

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Section: Pathological Changes In Rats Asmentioning

confidence: 99%

Pathological Changes in Rats as a Result of Treatment with Monocrotaline

Schoental¹,

Head²

1955

Br J Cancer

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“…Paper electrophoresis was carried out by a modification and combination of several methods (15)(16)(17). A vapor tight plastic chamber was employed and the experiments were conducted at 20 to 250 C. using veronal-citrate buffer pH 8.6, r/2 0.05; a rectangular sheet of Whatman 3 1662…”

Section: Methodsmentioning

confidence: 99%

Protein-Lipid Relationships in Human Plasma. III. In Pregnancy and the Newborn1

Russ¹,

Eder²,

Barr³

1954

J. Clin. Invest.

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This study of pregnant women and their newborn infants was undertaken as a part of a more extensive investigation of factors that modify the interrelations of proteins and lipids in human plasma (1-3).The composition of maternal and fetal blood has been examined by many observers who, however, have usually focussed attention either on proteins or lipids. Electrophoretic studies of proteins have been made by Longsworth, Curtis, and Pembroke (4) who analyzed both serum and plasma; by Lagercrantz (5) and Moore, DuPan, and Buxton (6) who examined only serum; and by Macy and Mack (7) whose monograph was devoted to an inquiry concerning plasma proteins in human reproduction. There is consensus that maternal plasma has less than normal concentration of albumin and a greater than normal concentration of alpha and beta globulins while gamma globulins are unchanged or only slightly decreased. These electrophoretic analyses of the cord plasma have shown lower than normal concentration of total protein which is chiefly attributable to a marked reduction in the concentration of alpha and beta globulins. Numerous investigators (8-10) have shown that in maternal blood the concentration of cholesterol and phospholipids is greater than normal while in blood from the umbilical cord at the time of birth it is notably reduced (11-13).In the present study, utilization of the microfractionation method No. 10 of Cohn and his coworkers (14) METHODSThe microfractionation method of Cohn and his coworkers (14) was used for separation of proteins. In this study the procedure was carried out in only three stages with isolation of Fraction IV + V + VI, Fraction II, and Fraction I + III. Analyses for protein, cholesterol, and phospholipids were made on the unfractionated plasma and each of its fractions. Because of the extremely small amounts of phospholipids in Fraction II, the determination was omitted.Details of the procedure involving fractionation and the analytical methods for proteins, cholesterol, and phospholipids have been presented in a previous publication (1). They have not been significantly modified for the present work.The distribution of protein components in the various fractions was studied electrophoretically by the moving boundary method and also by paper ionophoresis which was adapted to the purpose of identifying lipoproteins. The moving boundary method was performed on ACD plasma and the three fractions using an Aminco Stern apparatus with a standard analytical cell. Details of this procedure and the preparation of samples prior to electrophoresis have been described in a previous publication. (1).Paper electrophoresis was carried out by a modification and combination of several methods (15)(16)(17). A vapor tight plastic chamber was employed and the experiments were conducted at 20 to 250 C. using veronal-citrate buffer pH 8.6, r/2 0.05; a rectangular sheet of Whatman 3 1662

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“…In the experiment of Chart 2, for example, 8 implanted mice of a group not shown in the chart were each given on days 4,5,6,7,8,9, and 10, 0.5 cc. of guinea pig serum that had been heated at 56°C.…”

Section: Ckaracteristics Of Tke Effective Constituent Of Guinea Pig Smentioning

confidence: 99%

“…for the separation of the "midpiece" and "endpiece" components of complement from the serum of human beings (4). In addition to the inhibition tests, electrophoretic analyses were made of the two fractions, a modification of the falter paper method of Flynn and De Mayo being employed (5). I These analyses showed that the midpiece fractions, which had been redissolved in 0.9 per cent NaC1 solution buffered at pH 7.3, were comprised of components having electrophoretic mobilities corresponding with those of the gamma, beta, and alpha 1 Dr. Goetz Richter generously made the electrophoretic analyses here mentioned and those in the succeeding experiments as weli.…”

Section: Ckaracteristics Of Tke Effective Constituent Of Guinea Pig Smentioning

confidence: 99%

Regression of Transplanted Lymphomas Induced in Vivo by Means of Normal Guinea Pig Serum

Kidd

1953

Journal of Experimental Medicine

114

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In an extension of the experimental studies recorded in an associated paper; attempts were made to isolate and characterize the constituent of guinea pig serum responsible for inducing regression of transplanted lymphomas in vivo. The active material was precipitated readily from the whole serum, along with some of the globulins, by means of ammonium sulfate in concentrations of 2.0 molar or greater; it withstood heating at 56°C. for 20 or 30 minutes, but was inactivated upon heating at 66°C. for similar periods; it was completely inactivated by chymotrypsin in concentrations of 1 or 2 mg./cc. during 6 hours at 37°C. Furthermore, the inhibitory effects of small amounts of the guinea pig serum in vivo were enhanced upon admixture with immune sera prepared by injecting the lymphosarcoma cells into rabbits. The facts as a whole suggest that the active material is a protein, and that it may be one or another of the components of complement; yet they do not suffice to establish its identity. Microscopic studies showed that the cells of subcutaneous lymphomas rapidly died and were resorbed following injections of relatively large amounts of guinea pig serum intraperitoneally into mice carrying them, while similar changes followed more gradually after repeated injections of smaller amounts of guinea pig serum. No changes referable to the guinea pig serum were seen in the normal tissues or organs of mice receiving it. Mouse lymphoma cells, suspended artificially as individuals in a physiological saline solution, regularly remained viable following incubation in vitro in mixture with guinea pig serum during 6 hours at 37°C. The finding provides strong evidence that the regression of lymphomas that follows injection of guinea pig serum in vivo is brought about through some reaction in which the guinea pig serum and the host both participate. Some of the implications of the findings are discussed.

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Micro-Electrophoresis of Protein on Filter-Paper*1

Cited by 268 publications

References 7 publications

Pathological Changes in Rats as a Result of Treatment with Monocrotaline

Pathological Changes in Rats as a Result of Treatment with Monocrotaline

Protein-Lipid Relationships in Human Plasma. III. In Pregnancy and the Newborn1

Regression of Transplanted Lymphomas Induced in Vivo by Means of Normal Guinea Pig Serum

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