Micro-CT protocols for scanning and 3D analysis of Hexaplex trunculus during its different life stages

Abstract

Micro-computed tomography (micro-CT) is a high-resolution 3D-imaging technique which is now increasingly applied in biological studies focusing on taxonomy and functional morphology. The creation of virtual representations of specimens can increase availability of otherwise underexploited and inaccessible samples. The 3D model dataset can be also further processed through volume rendering and morphometric analysis. The success of micro-CT as a visualisation technique depends on several methodological manipulations, including the use of contrast enhancing staining agents, filters, scanning mediums, containers, exposure time and frame averaging. The aim of this study was to standardise a series of micro-CT scanning and 3D analysis protocols for a marine gastropod species, Hexaplex trunculus. The analytical protocols have followed all the developmental stages of this gastropod, from egg capsules and embryos to juveniles and adults.

3D printed membranes of polylactic acid and graphene oxide for guided bone regeneration

“…Approximately 700 micro-CT images were generated and analyzed using a CT Analyser. 56 The variables such as bone mineral density (BMD), trabecular bone volume to total volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were measured and evaluated to compare between groups (control, collagen, GO-PLA, and PLA).…”

Cyasterone has a protective effect on steroid-induced Osteonecrosis of the femoral head

“…Micro-CT parameters and indicators: The scanning voltage is 80 kV, the electric current is 500μA, and the scanning resolution is 10.44μm. After scanning, mimics software was used for 3D reconstruction with voxels of 1024μm×1024μm×1024μm [ 20 ]. The detection parameters include tissue volume (TV, Tissue Volume), bone volume (BV, Bone Volume); the Connection Density (Connectivity Density, Conn.D.…”

“…Micro-CT parameters and indicators: The scanning voltage is 80 kV, the electric current is 500μA, and the scanning resolution is 10.44μm. After scanning, mimics software was used for 3D reconstruction with voxels of 1024μm×1024μm×1024μm [20].…”