3D printed membranes of polylactic acid and graphene oxide for guided bone regeneration

Jang, Hee Jeong; Kang, Moon Sung; Kim, Won-Hyeon; Jo, Hyo Jung; Lee, Sung-Ho; Hahm, Eun Jeong; Oh, Jung Hyun; Hong, Suck Won; Kim, Bongju; Han, Dong-Wook

doi:10.1039/d3na00112a

Cited by 9 publications

(4 citation statements)

References 57 publications

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“…The digital imaging system (CELENA S, Logos Biosystems, Korea) were used for z-stack fluorescence images. From the obtained images, cell viability was calculated using Image J as follows (5) [ 31 , 32 ].

…”

Section: Methodsmentioning

confidence: 99%

Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting

Moon,

Park,

Cha

et al. 2024

Materials Today Bio

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…”

Section: Methodsmentioning

confidence: 99%

Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting

Moon,

Park,

Cha

et al. 2024

Materials Today Bio

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“…Furthermore, the addition of BG enhances cell viability and the expression of endothelial marker genes in vitro, as demonstrated on human umbilical vein endothelial cells (HUVECs), indicating a positive effect on angiogenesis [ 124 ]. Pearl powder [ 125 ], graphene oxide [ 126 ], and cold argon plasma treatment [ 119 ] are all valuable supplements that can influence bone cell differentiation, proliferation, and scaffold strength.…”

Section: 3d Cell Culturesmentioning

confidence: 99%

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Górnicki,

Lambrinow,

Golkar-Narenji

et al. 2024

Nanomaterials

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Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible and can influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic scaffolds adds complexity to traditional cell cultivation methods. The most commonly used technique involves cultivating cells on a flat surface in a two-dimensional format due to its simplicity. A three-dimensional (3D) format can provide a microenvironment for surrounding cells. There are two main techniques for obtaining 3D structures based on the presence of scaffolding. Scaffold-free techniques consist of spheroid technologies. Meanwhile, scaffold techniques contain organoids and all constructs that use various types of scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels that are one of the most extensively studied forms of potential scaffolds for 3D culture up to 4D bioprinted biomaterials. 3D bioprinting is one of the most important techniques used to create biomimetic scaffolds. The versatility of this technique allows the use of many different types of inks, mainly hydrogels, as well as cells and inorganic substances. Increasing amounts of data provide evidence of vast potential of biomimetic scaffolds usage in tissue engineering and personalized medicine, with the main area of potential application being the regeneration of skin and musculoskeletal systems. Recent papers also indicate increasing amounts of in vivo tests of products based on biomimetic scaffolds, which further strengthen the importance of this branch of tissue engineering and emphasize the need for extensive research to provide safe for humansbiomimetic tissues and organs. In this review article, we provide a review of the recent advancements in the field of biomimetic scaffolds preceded by an overview of cell culture technologies that led to the development of biomimetic scaffold techniques as the most complex type of cell culture.

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“…PLA finds extensive applications in the pharmaceutical field, including sustained-release packaging [29], surgical sutures [30] and so on. Over the past decades, PLA has been extensively employed in bone tissue regeneration research due to its remarkable biological properties [31,32]. Zhou et al [33] demonstrated the successful design and fabrication of a 3D-printed scaffold material based on PLA, which effectively enhanced osteogenic ability in human fetal osteoblast cells (hFOB).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Osteogenic Activity and Antibacterial Properties of Graphene Oxide-Poly(Lactic Acid) Films for the Repair of Cranial Defects in Rats

Liu,

Lai,

et al. 2024

Coatings

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The failure of bone defect repair caused by bacterial infection is a significant clinical challenge. However, the currently utilized bone graft materials lack antibacterial properties, necessitating the development of bone repair materials with both osteoinductive and antibacterial capabilities. Graphene oxide (GO) has garnered considerable attention due to its distinctive physical, chemical, and biological characteristics. In this study, we prepared a graphene oxide-poly(lactic acid) (GO-PLA) film with exceptional biological properties. In vitro investigations demonstrated that the GO-PLA film substantially enhanced the adhesion and proliferation capacity of rat bone marrow mesenchymal stem cells (rBMSCs). Furthermore, we observed augmented alkaline phosphatase activity as well as increased expression levels of osteogenic genes in rBMSCs cultured on the GO-PLA film. Additionally, we evaluated the antibacterial activity of our samples using gram-positive Streptococcus mutans (Sm) and gram-negative Actinobacillus actinomycetemcomitans (Aa). Our findings revealed that GO doping significantly inhibited bacterial growth. Moreover, implantation experiments conducted on rat skull defects demonstrated excellent guided bone regeneration performance exhibited by the GO-PLA film. Overall, our results indicate that the GO-PLA film possesses outstanding osteogenic and antibacterial properties, making it a promising biomaterial for bone tissue regeneration.

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3D printed membranes of polylactic acid and graphene oxide for guided bone regeneration

Abstract: We fabricated graphene oxide (GO)-incorporated polylactic acid (PLA) (GO-PLA) films by using three-dimensional (3D) printing to explore the potential benefits of guided bone regeneration (GBR). Our results showed that the...

Cited by 9 publications

References 57 publications

Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting

Photo-/thermo-responsive bioink for improved printability in extrusion-based bioprinting

Biomimetic Scaffolds—A Novel Approach to Three Dimensional Cell Culture Techniques for Potential Implementation in Tissue Engineering

Enhanced Osteogenic Activity and Antibacterial Properties of Graphene Oxide-Poly(Lactic Acid) Films for the Repair of Cranial Defects in Rats

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