Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts

Chiang, Hsiu-Mei; Chen, Hsin-Chun; Lin, Tsen-Jung; Shih, I-Chen; Wen, Kuo‐Ching

doi:10.1016/j.fct.2012.08.018

Cited by 82 publications

(65 citation statements)

References 39 publications

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“…Repeated skin exposure to UVB can cause collagen breakdown resulting in an aged skin appearance such as wrinkle formation. The mechanism underlying UVB-induced collagen degradation is the generation of various reactive oxygen species (ROS) provoking the mitogenactivated protein (MAP) kinase pathway, which subsequently induces the expression and activation of matrix metalloproteinases (MMPs) in human skin fibroblasts (Moon et al 2008;Chiang et al 2012;Wen et al, 2012). These enzymes include collagenase-1 (MMP-1) and collagenase-3 (MMP-13), which are the primary mediators of connective tissue damage in skin following UV irradiation exposure (Ravanti et al 1999;Brennan et al 2003;Ahn et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Ethanol extract ofTerminalia chebulafruit protects against UVB-induced skin damage

Yakaew

Itsarasook

Ngoenkam

et al. 2016

Pharmaceutical Biology

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Context: The fruit of Terminalia chebula Retz. (Combretaceae) has been used for several therapeutic purposes in Thai folk medicines. Currently, the ethanol extracts containing antioxidant compounds have shown the ability to promote collagen synthesis.Objective: This purpose of this work was to study the effects of the ethanol extract from T. chebula fruit on the inhibition of cutaneous photodamage. Materials and methods: The viability of human skin fibroblasts after incubation with T. chebula at concentration 0.5-50 lg/mL for 24, 48 and 72 h was assessed by using sodium 3 0 -[(phenyl-amino)-carbonyl]-3,4,tetrazolium-bis(4-methoxy-6-notro)benzene-sulphonic acid hydrate (XTT). The levels of type I procollagen and matrix metalloproteinases (MMP)-1 and MMP-13 produced by UVB-irradiated fibroblasts were determined by ELISA. Skin thickness and collagen content caused by long-term UVB irradiation in male ICR mice were determined from haematoxylin and eosin stained tissue sections and spectrophotometric measurement of hydroxyproline. Results: The extract (0.5-50 lg/mL) had no effect on cell viability or morphology of the human fibroblasts. In vitro studies showed that the T. chebula extract reduced the UVB-induced MMP-1 and MMP-13 expression, whereas an increased production of type I procollagen was observed. In a UVB-irradiated animal model, male ICR mice with hair shaved were chronically exposed to UVB which lead to epidermal thickness and loss of hydroxyproline. However, these effects were fully prevented by the topical application of the T.

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Section: Introductionmentioning

confidence: 99%

Ethanol extract ofTerminalia chebulafruit protects against UVB-induced skin damage

Yakaew

Itsarasook

Ngoenkam

et al. 2016

Pharmaceutical Biology

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“…Since MMPs-induced collagenous matrix damage is a main phenomenon of aged skin, MMPs inhibition is thought be a useful method for skin anti-aging. It has been reported that natural product plays a role on MMPs down-regulation in UV-irradiated cells [2,3,13,15]. Polymethoxyflavonoids have been shown to exhibit a broad spectrum of pharmacological actions including anti-cancer, anti-inflammation, anti-oxidant and anti-obesity [5,7,14].…”

Section: Mmp-1 Expressionmentioning

confidence: 99%

“…MMPs are a family of matrix degrading enzymes consisting of different members with substrate specificity that play important roles in various destructive processes by remodeling extracellular matrix (ECM) [10]. Among these, MMP-1, known as collagenase, is a key enzyme involved in collagen degradation due to oxidative stress, which is mediated by mitogen activating protein kinase (MAPK) -extracellular signal-regulated kinase (ERK)/p38/JUN-N-terminal kinase (JNK) activation [3,8]. Especially, ERK and JNK are known to be involved in MMP-1 expression via activation of the MMP-1 promoter by the transcription factor AP-1 and NF-kB [2,4,8].…”

Section: Introductionmentioning

confidence: 99%

Effects of Citrus sunki Peel Extract on Matrix Metalloproteinase-1 Expression

Han¹,

Lee

2013

Journal of Life Science

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Flavonoids are one of the major components found in the peels of citrus fruits. Present evidence has suggested that polymethoxyflavonoids, including nobiletin and tangeretin isolated from Citrus sunki, have many biological properties, such as anti-inflammatory, anti-oxidant, and anti-obesity capabilities.Here, we investigated the effect of Citrus sunki peel extract and its possible mechanisms on oxidative stress-induced MMP-1 expression, a major marker of skin photoaging. H2O2 induced MMP-1 expression in a dose-and time-dependent manner. Extract of Citrus sunki peel (1-25 μg/ml) dose-dependently decreased MMP-1 mRNA levels. When H2O2 was combined with Citrus sunki peel extract, the phosphorylation of ERK was further decreased compared to a single treatment with H2O2 alone. Moreover, U0216, an MEK inhibitor, markedly prevented the production of MMP-1. These data suggest that Citrus sunki peel extract has demonstrated protective activity against oxidative damage on MMP-1 expression, and ERK MAP kinase may be involved.

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“…A aplicação destes compostos no desenvolvimento de produtos antienvelhecimento tem sido amplamente estudada, com destaque para os produtos que apresentam atividade antioxidante, anti-inflamatória, indutora da síntese de colágeno ou inibitória das MMP (Chiang et al, 2012).…”

Section: Produtos Naturais Antienvelhecimentounclassified

“…Parte dos efeitos apresentados por produtos naturais em cultura de células é atribuída à sua ação antioxidante, e é este tipo de atividade biológica que é encontrada na maioria dos produtos naturais utilizados em formulações cosméticas (Singh e Agarwal, 2009;Cronin e Draelos, 2010 contribuir para a redução de modificações na pele provocadas por enzimas envolvidas na remodelação do tecido conjuntivo (Aslam et al, 2006;Thring et al, 2009;Chiang et al, 2012;Ratz-Lyko et al, 2015).…”

Section: Produtos Naturais Antienvelhecimentounclassified

Avaliação da expressão gênica de colágeno em fibroblastos tratados com extratos de fungos endofíticos

Araújo¹

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Skin aging process is very complex and can be classified as intrinsic aging, primarily determined by genetic factors, and extrinsic aging, induced by nutritional and behavioral factors, such as chronic exposure to ultraviolet radiation, excessive alcohol consumption, smoking and nutritional disorders. Skin aging is characterized by morphological and functional changes, which can cause wrinkling, scaling, dryness, pigmentation abnormalities and skin cancer. Some of the disorders that affect the skin aging are mainly due to oxidative stress promoted by reactive oxygen species that are able to change the balance between synthesis and degradation of the dermal extracellular matrix components, favoring degradation rather than the synthesis process. Natural products protect skin from damage induced by oxidative stress and may act in important pathways in the skin aging. Among the possible sources for obtaining natural products it is possible to highlight the endophytic fungi, which are able to produce a great diversity of natural compounds. The objective of this study was to evaluate the influence of treatment with metabolites produced by two endophytic fungi isolated from Bauhinia variegate leaves on genic expression of collagen type I, metalloproteinase type 1 and tissue inhibitor of metalloproteinase type 1 in human dermal fibroblasts exposed to oxidative stress. The results show that both fungi were able to produce polyphenolic compounds and, more specifically, flavonoids. Treatment of fibroblasts with the extract produced by one of the endophytic fungi increased the expression of collagen type I gene, reduced the expression of metalloproteinase type 1 gene and inhibited metalloproteinase type 2 activity, suggesting that metabolites produced by this fungus can be benefic in prevent skin aging.

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Michelia alba extract attenuates UVB-induced expression of matrix metalloproteinases via MAP kinase pathway in human dermal fibroblasts

Cited by 82 publications

References 39 publications

Ethanol extract ofTerminalia chebulafruit protects against UVB-induced skin damage

Ethanol extract ofTerminalia chebulafruit protects against UVB-induced skin damage

Effects of Citrus sunki Peel Extract on Matrix Metalloproteinase-1 Expression

Avaliação da expressão gênica de colágeno em fibroblastos tratados com extratos de fungos endofíticos

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