Micellar liquid chromatography of plant growth regulators detected by derivative fluorometry

Sánchez, Francisco; Dı́az, A. Navas; Pareja, A. Garcı́a

doi:10.1016/0021-9673(95)00886-1

Cited by 23 publications

(5 citation statements)

References 20 publications

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“…Phytohormones are classified into four groups: auxins, gibberellins, cytokinins and inhibitors, and they may act in a different way to achieve the regulation of a range of physiological processes 5, 6. Auxins are a group of compounds that regulate plant development, affecting cell division and root formation 7.…”

Section: Introductionmentioning

confidence: 99%

QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS

Flores

Romero‐González

Vidal

2011

J of Separation Science

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A new method has been developed and validated for the simultaneous analysis of different phytohormones (auxins, cytokinins and gibberellins) in vegetables. The compounds were extracted using a QuEChERS-based method (acronym of quick, easy, cheap, effective, rugged and safe). The separation and determination of the selected phytohormones were carried out by ultra high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), using electrospray ionization source (ESI) in positive and negative ion modes. The method was validated and mean recoveries were evaluated at three concentration levels (50, 100 and 250 μg/kg), ranging from 75 to 110% at the three levels assayed. Intra- and interday precisions, expressed as relative standard deviations (RSDs), were lower than 20 and 25%, respectively. Limits of quantification (LOQs) were equal or lower than 10 μg/kg. The developed procedure was applied to seven courgette samples, and naphthylacetic acid, naphthylacetamide and benzyladenine were found in some of the analysed samples.

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Section: Introductionmentioning

confidence: 99%

QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS

Flores

Romero‐González

Vidal

2011

J of Separation Science

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“…The harvesting was carried out when the apex zone, the center zone, and the root reached 1, 0.5 and 2.5 cm, respectively, and were then stored at −40 °C before trituration and analysis. The extraction procedure and bulk purification of plant material were described in a previous work …”

Section: Methodsmentioning

confidence: 99%

“…Bile salts appear to be more effective than synthetic surfactants or cyclodextrins and greatly improve luminescence analysis. , In addition, because bile compounds increase separations, − they have been used in reversed-phase micellar liquid chromatography − and as ion-pair reagents for liquid chromatography …”

mentioning

confidence: 99%

Normal-Phase Liquid Chromatography of Plant Hormones Using Reversed Cholic Acid Micelles as the Mobile Phase

Dı́az¹,

Pareja²,

Sánchez³

1996

Anal. Chem.

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When several plant hormones with similar structures are present in a sample, conventional analytical methods are not sufficiently selective to effectively differentiate them. However, the excellent selectivity of micellar liquid chromatography allows similarly structured compounds to be easily differentiated. We used reversed cholic acid micelles dissolved in tetrahydrofuran as the mobile phase in normal-phase chromatography of seven plant hormones. Our results conformed closely to those predicted by the pseudophase micellar liquid chromatography theory. The chromatographic efficiencies were calculated from the peak width and the retention times, and we compared these values with those obtained by reversed-phase chromatography using sodium dodecyl sulfate mobile phase in water. To test the efficiency of this selective method for the analysis of plant tissues that may also contain contaminants, we applied it to homogeneous suspensions of spiked maize roots. Recoveries ranged from 91 to 110%, the relative standard deviations were between 0.46 and 10.03%, and the detection limits were between 0.08 and 0.80 micrograms g-1.

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“…In thin layer chromatography the product from the fluorescamine reaction 4 is observed under a UV lamp; this reagent has also been proposed for pre or postcolumn chromatographic derivatization [26][27][28] by measuring the fluorescence emission at 484 nm (excitation at 380 nm). Other authors have proposed ion-pair reverse-phase liquid chromatography using sodium cholate as an organic counter ion.…”

Section: Introductionmentioning

confidence: 99%

“…Other authors have proposed ion-pair reverse-phase liquid chromatography using sodium cholate as an organic counter ion. 29 Other non-chromatographic methods also used the fluorescamine reaction after liquid extraction 30 This paper presents the analytical determination of the pesticide Asulam based on its native fluorescence. The method was optimized in either a flow injection analysis (FIA) assembly or in batch.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence Determination of the Pesticide Asulam by Flow Injection Analysis

et al. 2006

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Micellar liquid chromatography of plant growth regulators detected by derivative fluorometry

Cited by 23 publications

References 20 publications

QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS

QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS

Normal-Phase Liquid Chromatography of Plant Hormones Using Reversed Cholic Acid Micelles as the Mobile Phase

Fluorescence Determination of the Pesticide Asulam by Flow Injection Analysis

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