Micellar electrokinetic chromatography with amperometric detection and off-line solid-phase extraction for analysis of carbamate insecticides

Santalad, Apichai; Zhou, Lin; Shang, Fengjun; Fitzpatrick, Dara; Burakham, Rodjana; Srijaranai, Supalax; Glennon, Jeremy D.; Luong, John H. T.

doi:10.1016/j.chroma.2010.06.024

Cited by 61 publications

(35 citation statements)

References 34 publications

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“…Several analytical techniques have been developed for the analysis of carbamates in various samples, such as spectrophotometry [4,5], voltammetry [6], gas chromatography (GC) [7], high-performance liquid chromatography (HPLC) [8][9][10], and capillary electrophoresis (CE) [11,12]. HPLC with a variety of detection systems, i.e., ultraviolet [8,[13][14][15], fluorescence [16,17], chemiluminescence [18], and mass spectrometry [19], is the preferred choice for analysis of carbamate pesticides.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an ultrasound-assisted surfactant-enhanced emulsification microextraction method for liquid chromatographic determination of carbamate residues in fruit juices

Vichapong¹,

Burakham²

2015

Acta Chromatographica

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Summary.A microextraction procedure based on ultrasound-assisted surfactantenhanced emulsification microextraction (UASEME) was optimized and validated for preconcentration of carbamate pesticides prior to fast determination using high-performance liquid chromatography (HPLC) with monolithic column. The nonionic surfactant (Tween 20) was used as an emulsifier, and chloroform was selected as an extraction solvent. The experimental parameters affecting the extraction efficiency, such as the kind, concentration, and volume of surfactant, the kind of extraction solvent and its volume, salt addition, ultrasonic extraction time, and centrifugation time, were optimized. Under the selected UASEME and HPLC conditions, separation of five carbamate pesticides (carbofuran, carbaryl, isoprocarb, methiocarb, and promecarb) was achieved within 10 min. The linearity of the method was obtained in the wide range of 2-5000 μg L −1 , with correlation coefficients of 0.9931-0.9970. The limits of detection were in the range of 0.4-7.0 μg L −1 . The relative standard deviations (n = 5) were less than 0.59% and 4.86% for retention time and peak area, respectively. The enrichment factors of the developed UASEME method were in the range of 33-166. The fruit juice samples (at fortified levels of 0.25, 0.50, and 1.00 mg L −1 ) were successfully analyzed, and the relative recoveries were obtained in the range of 81-115%.

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Section: Introductionmentioning

confidence: 99%

Development and validation of an ultrasound-assisted surfactant-enhanced emulsification microextraction method for liquid chromatographic determination of carbamate residues in fruit juices

Vichapong¹,

Burakham²

2015

Acta Chromatographica

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“…It was noted that the migration order for all pesticides remained unchanged over the range of pH values examined. It was also observed that there were no major changes in the extent to which the pesticides were not ionized at all pH values investigated []. Using a 25 mM phosphate buffer of pH 6.0, 7.0, 8.0, 9.0, and 10.0, EOF mobilities were 3.01, 4.91, 5.90, 7.27, and 7.96 × 10 −4 cm 2 V −1 s −1 , respectively.…”

Section: Resultsmentioning

confidence: 95%

Analysis of carbofuran, carbosulfan, isoprocarb, 3‐hydroxycarbofuran, and 3‐ketocarbofuran by micellar electrokinetic chromatography

Hsu

Chiu

2012

J of Separation Science

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We developed an analytical method for the detection and quantitation of five pesticides and some of their metabolites - 3-hydroxycarbofuran, 3-ketocarbofuran, carbofuran, carbosulfan, and isoprocarb - using micellar electrokinetic chromatography coupled with a UV-Vis detector. The optimum separation conditions were 20 mM phosphate buffer (pH 8.0) containing 15 mM sodium dodecyl sulfate. The detection wavelength was set at 200 nm and the applied voltage was 12.5 kV. Under these conditions, baseline separation of five pesticides was achieved in 15 min, and the detection limits (S/N = 3) of 3-hydroxycarbofuran, 3-ketocarbofuran, carbofuran, carbosulfan, and isoprocarb were 0.3, 0.3, 0.3, 4.0, and 0.3 μM, respectively. The linear ranges for 3-hydroxycarbofuran, 3-ketocarbofuran, carbofuran, and isoprocarb were between 1.0 and 50.0 μM and that for carbosulfan was between 10.0 and 100.0 μM, with R(2) larger than 0.995. When applied to the analysis of a carbofuran-spiked rice sample, this approach yielded results with excellent repeatability (3.3%, n = 5), reproducibility (4.5%, n = 5), separation efficiency (>2.1 × 10(4) theoretical plates), and recovery (95.5 ± 1.4%, n = 5).

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“…To date, some sensitive methods have been developed for carbamates with CE methods such as CZE and MEKC . For the AD scheme, few work was studied in CE with a pre‐column hydrolysis, which was high‐time consuming and not suitable for on‐line rapid diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Sensitive amperometric detection for capillary electrophoresis of phenol carbamates with in‐line thermal hydrolysis strategy

et al. 2019

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A strategy on amperometric detection for CZE of phenol carbamates as model analytes with a facile in‐line thermal hydrolysis was presented, in which a thermal hydrolysis, subsequent CZE separation and final column‐end amperometric detection were accomplished in an intact capillary. Key parameters of hydrolysis dynamics of carbamates and electrochemical detection of the hydrolysates were studied, as well as electrophoretic conditions. Under the optimal conditions, the capillary was utilized as chambers for in situ hydrolysis, CZE separation, and electrochemical detection. The successive separation of hydrolysates of five carbamates (propoxur, carbofuran, 3‐OH‐carbofuran, carbaryl and bendiocarb) were achieved within 17 min. Applied to vegetable samples, the recoveries of carbamates fortified at 0.02 and 0.05 mg/kg were ranging in 88–107.2 and 86.3–107.3%, respectively. The success in the implementation of such a scheme resulted in a simple instrument as compared with those current analytical methods with post‐column derivization or pre‐column hydrolysis, or online enrichment in chip, respectively. This protocol might possess a potential utility for the sensitive amperometric detection of phenol carbamates.

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Micellar electrokinetic chromatography with amperometric detection and off-line solid-phase extraction for analysis of carbamate insecticides

Cited by 61 publications

References 34 publications

Development and validation of an ultrasound-assisted surfactant-enhanced emulsification microextraction method for liquid chromatographic determination of carbamate residues in fruit juices

Development and validation of an ultrasound-assisted surfactant-enhanced emulsification microextraction method for liquid chromatographic determination of carbamate residues in fruit juices

Analysis of carbofuran, carbosulfan, isoprocarb, 3‐hydroxycarbofuran, and 3‐ketocarbofuran by micellar electrokinetic chromatography

Sensitive amperometric detection for capillary electrophoresis of phenol carbamates with in‐line thermal hydrolysis strategy

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