Micellar electrokinetic capillary chromatography, high performance liquid chromatography and nuclear magnetic resonance—three orthogonal methods for characterization of critical drugs

Deubner, Ralph; Holzgrabe, Ulrike

doi:10.1016/j.jpba.2004.01.015

Cited by 30 publications

(19 citation statements)

References 10 publications

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“…In contrast, a MEKC method in connection with derivatization with orthophthaldialdehyde (derivatization cf. to the "old" EP method [24]) was shown to be highly efficient, fast (less than 15 min) and selective and, thus, superior to the HPLC method [20,24]. This also holds true for tobramycin and other aminoglycosides [25].…”

Section: Examples Of Ce Superiority Over Hplcmentioning

confidence: 98%

Why not using capillary electrophoresis in drug analysis?

et al. 2006

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CE and related methods are well-established techniques in the analysis of biomolecules, such as DNA and proteins. Even though CE is a rather good alternative to HPLC for the evaluation of the impurity profile and the enantiomeric purity of a drug, it is rarely applied. This might be due to the reservation of national licensing authorities and the pharmacopoeia commissions for several reasons. In this review containing some experimental data we report on several drug examples which demonstrate the superiority of CE over HPLC in special cases, i.e., in the analysis of antibiotics, amino acids and peptides, and the determination of enantiomeric purity. However, in order to make the CE techniques more suitable for pharmacopoeial purposes the general methods describing separation methods have to be complemented with the adjustment of the electrophoretic conditions being necessary to satisfy the system suitability criteria without fundamentally modifying the methods. Taken together CE should be more often applied in drug quality control.

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Section: Examples Of Ce Superiority Over Hplcmentioning

confidence: 98%

Why not using capillary electrophoresis in drug analysis?

et al. 2006

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“…Because of the lack of UV chromophore groups in these antibiotics, derivatization is usually required prior to UV-detection. Thus, precolumn derivatization with 1,2-phthalic dicarboxaldehyde (OPA) was employed to analyze amikacin, tobramycin, gentamicin, sisomicin, and netilmicin using borate buffer at pH 10 in presence of the bile salt deoxycholate and the native b-CD used to enlarge the separation window [15,16]. Under these experimental conditions, the separation of the four major components of gentamicin in a commercial sample from the derivatization agent (OPA) and an internal standard (IS) was achieved and it is illustrated in Fig.…”

Section: Aminoglycoside Antibioticsmentioning

confidence: 99%

“…Then, samples were derivatized with OPA in presence of methanol. These solutions after being vortexed and heated in a water bath at 407C for exactly 4 min were diluted with methanol and cooled to room temperature prior to the injection in the CE system [16].…”

Section: Antibiotics In Pharmaceutical Preparationsmentioning

confidence: 99%

Recent advances in the analysis of antibiotics by capillary electrophoresis

García-Ruiz

Marina

2006

Electrophoresis

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Recent advances in the analysis of antibiotics by capillary electrophoresisIn this review, the main aspects related to the separation of different groups of antibiotics by CE as well as the different applications reported in the literature from the beginning 2003 till May 2005 will be provided to the readers. Firstly, the experimental conditions employed to achieve the analysis of antibiotics by CE are given. Then, the main applications performed in the pharmaceutical, clinical, food, and environmental fields have been reviewed making emphasis on sample preparation requirements needed in each case. Finally, the main conclusions and future prospects in this field are presented. Although HPLC is mainly used for the analysis of antibiotics by separation techniques, CE is being increasingly employed due to its favorable characteristics (high efficiency, large flexibility, and low consumption of samples and reagents). In addition, CE is being used in routine analysis because it allows obtaining appropriate analytical characteristics and good quantitative results. The analysis of antibiotics by CE is mainly included in two different working modes: (i) CZE where a separation buffer without or with additives is used for the separation of ionic or ionogenic antibiotics based on their different electrophoretic mobilities, and (ii) MEKC where a micellar system (surfactant at a concentration higher than its CMC) is added to the separation buffer to perform the separation of neutral and/or ionic or ionogenic antibiotics based on the generation of a pseudostationary phase in which analyte partition takes place. Although much less used, CEC and nonaqueous CE (NACE) have also been used for the analysis of antibiotics [2]. KeywordsIn order to provide to the readers an updated view of the separation conditions as well as the different applications reported in the analysis of antibiotics using CE as separation technique, this review covers the literature dealing on the analysis of antibiotics by CE from the beginning of 2003 till May 2005. Literature published before this date on this subject has already been reviewed by Flurer [2][3][4][5]. The experimental conditions employed to achieve the analysis of antibiotics by CE are first presented in this article. Section 3 of this work describes the main applications performed in the pharmaceutical, clinical, food, and environmental fields making emphasis on sample preparation requirements needed in each case. Finally, the main conclusions and future prospects in this field are presented. Analysis of antibiotics by CE

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“…By far, the most reported surfactant is the anionic sodium dodecyl sulfate (SDS). Sodium deoxycholate (DOC) and sodium cholate, which are bile salts, were also reported in [2003][2004] [17][18][19][20]. Cationic surfactants used in MEKC including CTAB continue to be reported [21] as do zwitterionic surfactants: CHAPS [22,23] and 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO) [23].…”

Section: Surfactants and Additivesmentioning

confidence: 99%

“…Drug determinations have been accomplished in plasma [117,138,141,142], urine [60,68,81], serum [79,117,[143][144][145], cerebral spinal fluid [141], and cell lines [125]. Impurity (and purity) analysis of drugs [18,59], preparations [146], and tablets [102,[147][148][149] have also been demonstrated with MEKC. Other reports have demonstrated means of determining metabolites [60,68,81,117,125], degradation products [92] and constituent analysis [65,94,150,151].…”

Section: Pharmaceuticalsmentioning

confidence: 99%

Recent advances in micellar electrokinetic chromatography

2005

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This review contains nearly 200 reference citations, and covers advances in electrokinetic capillary chromatography based on micelles, including stabilized micelle complexes, polymeric and mixed micelles from 2003-2004. Detection strategies, analyte determinations, and applications in micellar electrokinetic capillary chromatography (MEKC) are discussed. Information regarding methods of analyte concentration, analyte specific analyses, and nonstandard micelles has been summarized in tabular form to provide a means of rapid access to information pertinent to the reader.

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Micellar electrokinetic capillary chromatography, high performance liquid chromatography and nuclear magnetic resonance—three orthogonal methods for characterization of critical drugs

Cited by 30 publications

References 10 publications

Why not using capillary electrophoresis in drug analysis?

Why not using capillary electrophoresis in drug analysis?

Recent advances in the analysis of antibiotics by capillary electrophoresis

Recent advances in micellar electrokinetic chromatography

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