Micellar electrokinetic capillary chromatography as an alternative method for determination of hydrocortisone and its most important associated compounds in local pharmaceutical preparations

Abstract

SummaryA new, simple, and accurate micellar electrokinetic chromatographic (MEKC) method is clescribed for quantification of hydrocortisone, hydrocortisone hemisuccinate, hydrocortisone acetate, nystatin, oxytetracychne, Zn-bacitracin, polymyxin B, and hdocaine in ocular and cutaneous pharmaceutical products. The separation was performed at 25 ~ and 25 kV, with 15 mM phosphate + 15 mM borate buffer, pH 8.2, and 60 mM sodium dodecylsulfate (SDS) in 10:1 (%, v/v) methanol-water as background electrolyte. Under these conditions the analysis time is approximately 23 min. The method has been used for quantification of these compounds in different commercial pharmaceutical products and gave good results when compared with reference spectrophotometric and HPLC methods.

Analysis of antibiotics by capillary electrophoresis

“…The LOQs were 19.0 mg/L for polymyxin, 8.5 mg/L for bacitracin and 6.5 mg/L for oxytetracycline. It was demonstrated that this method could be used for the analysis of eardrops, ointments and tablets containing bacitracin, polymyxin, oxytetracycline and/or other components; however, it was acknowledged that the extraction step was not optimized for each product [25].…”

“…Prednisolone, prednisolone acetate, zinc bacitracin, sulfacetamide sulfate, phenylephrine and naphazoline nitrate were separated in a 5 mM phosphate-5 mM borate buffer, pH 8.2, containing 50 mM SDS and 3% v/v CH 3 CN, with detection at 205 nm. For sulfacetamide, the method is linear from 0.3 to 32.0 mg/mL, with an LOQ of 0.3 mg/mL, and an LOD of 0.09 mg/mL [25]. The determination of the amount of sulfacetamide present in both eye drops and ointment was demonstrated [25].…”

“…MEKC was used for the determination of sulfacetamide sulfate in a mixture of compounds that can be found in ocular or nasal preparations [25]. Prednisolone, prednisolone acetate, zinc bacitracin, sulfacetamide sulfate, phenylephrine and naphazoline nitrate were separated in a 5 mM phosphate-5 mM borate buffer, pH 8.2, containing 50 mM SDS and 3% v/v CH 3 CN, with detection at 205 nm.…”

“…Polymyxin B, zinc bacitracin and oxytetracycline were separated from hydrocortisone and related compounds using MEKC [25]. A buffer containing 60 mM SDS, 10% v/v MeOH, 15 mM phosphate, 15 mM borate, pH 8.2, produced single peaks for both polymyxin and bacitracin.…”

Comparative Study of New Spectrophotometric Methods; An Application on Pharmaceutical Binary Mixture of Ciprofloxacin Hydrochloride and Hydrocortisone

“…The methods reported for HYD such as UV spectrophotometry 14 , HPLC [15][16][17] , micellar electrokinetic capillary chromatography 18,19 and capillary electrophoresis 20 . Simultaneous determination of CIP and HYD has been reported in the literature by HPLC method 21 and chemometric method 22 .…”