Micellar capillary electrophoresis of the ecdysteroids

Determination of 20-hydroxyecdysone content by thin-layer chromatography and micellar electrokinetic chromatography

“…The limit of detection for 20-hydroxyecdysone was found to be under 20 pg using MEKC, but about 10 to 100 ng using TLC. Major differences may be given by the detection wavelength 254 nm for TLC but 240 nm with MEKC in the literature [13,14] and 214 nm in our experiments. It is important to note that 254 nm or 240 nm may provide more selective detection than 214 nm, however, the shortcoming of the latter is counterbalanced by the good separation that generates single well separated peaks without overlap.…”

“…Large et al [13] and Davis et al [14] have recently introduced MEKC separation of some ecdysteroids from plant extracts. We have modified their method to decrease the separation time (from 30 min to 10 min) while also improving the resolution between 20-hydroxyecdysone and polypodine B.…”

Capillary Electrophoresis

“…MEKC was applied to the separation of closely related ecdysteroids (polyhydroxylated insect hormones) both as pure standards and in the extracts of plants and insect eggs. The quality of the result was found to be critically dependent on the solvent in which the sample was applied and the purity of the extracts (417).…”