Introduction to CE and the Use of CE in Pharmaceutical Analysis

Altria, Kevin D.

doi:10.1007/978-3-322-85011-9_1

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…Both the electroosmotic and electrophoretic velocities are directly proportional to the fi eld strength, so the use of the highest voltages possible will result in the shortest times for the separation, the limiting factor being the capacity of the electrophoretic system to dissipate the heat generated by the Joule eff ect. Th e electrophoretic mobility and the EOF are infl uenced strongly by viscosity and consequently temperature [27,28].…”

Section: Discussionmentioning

confidence: 99%

Development of a Capillary Electrophoresis Method for the Separation of Fluoroquinolone Derivatives in Acidic Background Electrolyte

Rusu

Hancu

Gyéresi

2014

Acta Medica Marisiensis

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Introduction: Antibacterial quinolones class comprises a series of synthetic antibacterial agents, following the model of nalidixic acid. Because of their common 6-fl uorosubtituent on the quinolone ring, fl uroquinolones are the most potent analogues with extended spectrum of activity and great pharmacokinetic properties. The applicability of capillary zone electrophoresis for the separation of fl uoroquinolones in acidic background electrolyte has been studied, our aim being the development of a capillary zone electrophoresis method for the simultaneous separation of six fl uoroquinolones and also to optimize the analytical conditions. The six studied fl uoroquinolones were ciprofl oxacin, enoxacin, enrofl oxacin, moxifl oxacin, ofl oxacin and sarafl oxacin. Material and methods: Preliminary, we studied the electrophoretic behavior of six fl uoroquinolones in an acidic pH, which highlighted the possibility of developing a separation method in this area of pH. Electrophoretic parameters infl uencing separation performance were studied and optimized. Results: A fast and reliable method has been developed, using a background electrolyte containing 100 mM phosphoric acid and the following conditions: applied voltage: + 25 kV, temperature: 20°C, injection pressure 30 mbar -5 sec, UV detection at 280 nm, capillary: 60 cm (52 cm effective length) x 50 μm, analyte concentration: 167 μg/ml. The separation of the studied fl uoroquinolones was achieved in less than 8 minutes. Conclusions: Capillary zone electrophoresis using an acidic background electrolyte proved to be an effi cient tool in the separation of fl uoroquinolones from different generations. Also the proposed methods are particular environment-friendly replacement and improvement of a common high performance liquid chromatography determination with rapid analysis time without using any organic solvents.

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Section: Discussionmentioning

confidence: 99%

Development of a Capillary Electrophoresis Method for the Separation of Fluoroquinolone Derivatives in Acidic Background Electrolyte

Rusu

Hancu

Gyéresi

2014

Acta Medica Marisiensis

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Capillary electrophoresis for the analysis of small‐molecule pharmaceuticals

2006

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This paper reviews the application of CE to the analysis of small-molecule pharmaceuticals. The areas of pharmaceutical analysis covered are enantiomer separation, the analysis of small molecules such as amino acids or drug counter-ions, pharmaceutical assay, determination of related substances and physicochemical measurements such as log P and pK(a) of compounds. The different electrophoretic modes available and their advantages for pharmaceutical analysis are described. Recent applications of CE for each subject area are tabulated with electrolyte details.

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Introduction to CE and the Use of CE in Pharmaceutical Analysis

Cited by 2 publications

References 43 publications

Development of a Capillary Electrophoresis Method for the Separation of Fluoroquinolone Derivatives in Acidic Background Electrolyte

Development of a Capillary Electrophoresis Method for the Separation of Fluoroquinolone Derivatives in Acidic Background Electrolyte

Capillary electrophoresis for the analysis of small‐molecule pharmaceuticals

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