MICA*055: a new allele with eight GCT repeats in the exon 5 microsatellite

Glas, J.; Maier, Kristin; Wetzke, Martin; Henninger, Michelle; Weiss, E.H.; Folwaczny, Mathias

doi:10.1111/j.1399-0039.2008.01098.x

Cited by 5 publications

(2 citation statements)

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“…The microsatellite polymorphisms in exon 5 of MICA , in intron 1 of MICB and in C1 _ 4 _ 1 were genotyped using polymerase chain reaction (PCR) amplification and fragment analysis. For analysis of MICA‐TM, MICB‐C1_2_A and C1_4_1, the primers were used as described previously (9, 22). For MICA‐TM the forward primer was 5′‐labelled with the fluorescent dye JOE, the range of the PCR product was 92–107 bp.…”

Section: Methodsmentioning

confidence: 99%

“…Microsatellite polymorphisms distinguish these variants. In exon 5 of the MICA locus, coding for the transmembrane segment of the MICA molecule, a GCT trinucleotide microsatelllite polymorphism has been described with five frequent (A4, A5, A5.1, A6 and A9) and three rare alleles (A7, A8 and A10) (7–9). Different alleles of the exon 5 MICA‐TM polymorphism have been associated with ulcerative colitis and Behcet disease (10, 11).…”

Section: Introductionmentioning

confidence: 99%

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Impact of MICA‐TM, MICB‐C1_2_A and C1_4_1 microsatellite polymorphisms on the susceptibility to chronic periodontitis in Germany

2011

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The major histocompatibility complex class I chain-related gene A (MICA)-TM exon 5 trinucleotide polymorphism, the MICB-C1_2_A intron 1 dinucleotide polymorphism and the tetranucleotide polymorphism C1_4_1 located in the major histocompatibility complex class I region on chromosome 6 were shown to influence various chronic inflammatory conditions. We investigated the association of these microsatellite polymorphisms with chronic periodontitis, a highly prevalent oral inflammatory disease in 389 periodontitis patients and 771 healthy controls with South German genetic background. Genotyping of the MICA-TM, MICB-C1_2_A and C1_4_1 microsatellite polymorphisms was performed by PCR amplification and fragment analysis. Global frequency distribution of MICB-C1_2_A (P = 0.006) and C1_4_1 (P = 0.028) alleles was significantly different between both study groups. Allele-specific analysis revealed that the MICA-TM allele A5 was more prevalent among male periodontitis patients [P = 0.0001; odds ratio (OR) 2.17, 95% confidence interval (CI) 1.55-3.03]. In C1_4_1 allele, three was significantly higher in healthy controls (P = 0.006; OR 0.74, 95% CI 0.60-0.91). Two haplotypes (MICA:A5-C1_4_1:5; P = 0.002; OR 2.63, 95% CI 1.46-4.74 and MICB:CA16-C1_4_1:3; P = 0.014; OR 0.68, 95% CI 0.50-0.92) showed significant differences between periodontitis patients and controls. The MICA-TM, MICB-C1_2_A and C1_4_1 microsatellite polymorphism seem to influence the individual susceptibility to chronic periodontitis in patients with German genetic background.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%