Mevalonate pathway genes expressed in chilli CM334 inoculated with Phytophthora capsici and infected by Nacobbus aberrans and Meloidogyne enterolobii

Villar-Luna, Edgar; Rojas-Martínez, Reyna Isabel; Reyes-Trejo, Benito; Gómez-Rodríguez, Olga; Zavaleta-Mejía, Emma

doi:10.1007/s10658-016-1142-0

Cited by 7 publications

(6 citation statements)

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“…El riego se realizó cada 24 h, y la fertilización quincenalmente con Nitrofoska TM 12-12-12 (3.1 g por litro de agua). Para la inoculación de las plantas con N. aberrans s.l., el inóculo se obtuvo de raíces agalladas de jitomate (cultivo monoxénico) (población del Colegio de Postgraduados Campus Montecillo, Estado de México, México) (Villar-Luna et al, 2017). La extracción de huevos se realizó según Hussey y Barker (1973), y la obtención de J 2 fue con la técnica de Baermann usando placas de Petri e incubando los huevos en agua esteril a 25±1 °C.…”

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“…Irrigation was applied every 24 h, and fertilization using Nitrofoska TM 12-12-12 (3.1 g per liter of water) every two weeks. For the inoculation of the plants with N. aberrans s.l., the inoculum was obtained from galled tomato roots (monoxenic culture) (population from Colegio de Postgraduados Campus Montecillo, State of Mexico, Mexico) (Villar-Luna et al, 2017). Egg extraction was performed according to Hussey and Barker (1973), and J 2 was obtained by applying Baermann's technique, that is, using Petri dishes and incubating the eggs in sterile water at 25±1 °C.…”

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confidence: 99%

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Aptitud de cinco poblaciones de jitomate silvestre (Solanum lycopersicum var. cerasiforme) como hospedante del nematodo Nacobbus aberrans sensu lato

Cazares-Álvarez

Méndez-Inocencio

Zepeda‐Jazo

et al. 2019

RMF

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Nacobbus aberrans sensu lato es uno de los nematodos más importantes en el cultivo de jitomate. Para su manejo, es de importancia el estudio de estrategias sostenibles como la resistencia genética, comúnmente encontrada en parientes silvestres de los cultivos. Al respecto, surgió el interés por conocer la respuesta de poblaciones locales (Michoacán, México) de jitomate silvestre (Solanum lycopersicum var. cerasiforme) a la inoculación con juveniles de segundo estadio de N. aberrans s.l. Se montaron tres experimentos (E1, E2, y E3). Se evaluaron cinco genotipos silvestres y uno cultivado (cv. Río grande). A los 7 y 21 días posteriores a la inoculación (dpi) se evaluó el número de nematodos dentro de la raíz, y a los 45 y 60 dpi el agallamiento de raíces. En E1 y E2, a 7 y 21 dpi se encontraron los estadios J2 y J3-J4. En los tres experimentos ocurrió agallamiento, y a 60 dpi la producción de huevos fue evidente. N. aberrans s.l. invadió las raíces, y completó su desarrollo y reproducción. Estos resultados revelan la aptitud de las cinco poblaciones mexicanas de jitomate silvestre como hospedantes del nematodo, y sugieren la necesidad de explorar otras solanáceas silvestres como posibles fuentes de resistencia al nematodo.

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confidence: 99%

Aptitud de cinco poblaciones de jitomate silvestre (Solanum lycopersicum var. cerasiforme) como hospedante del nematodo Nacobbus aberrans sensu lato

Cazares-Álvarez

Méndez-Inocencio

Zepeda‐Jazo

et al. 2019

RMF

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“…The inoculum of N. aberrans was obtained from galled tomato roots collected from a naturally infested plot. The extraction of eggs was performed following the methodology described by Vrain (1977) and second-stage juveniles (J2) were obtained according to Villar-Luna et al, (2009). Plants with four to five true leaves were inoculated with 3 000 J2 per plant.…”

Section: Chilli Plants and Inoculation With N Aberrans And P Capsicimentioning

confidence: 99%

“…Plants with four to five true leaves were inoculated with 3 000 J2 per plant. P. capsici inoculum was prepared as indicated by Villar-Luna et al (2009) and each plant was inoculated with 500 000 zoospores. As a reference of the pathogenicity of P. capsici and inoculation efficacy, chili plants cv.…”

Section: Chilli Plants and Inoculation With N Aberrans And P Capsicimentioning

confidence: 99%

Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants

Villar-Luna¹,

García-Espinoza²,

Gómez-Rodríguez³

et al. 2015

Helminthologia

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SummaryCapsicum annuum L. CM334 is susceptible to Nacobbus aberrans but highly resistant to Phytophthora capsici. Resistance to P. capsici is associated with the over-expression of various defense genes such as those encoding pathogenesis-related proteins. The transcriptional alterations of defense-related genes were determined in galls induced by N. aberrans (Na) in CM334 chili roots. Transcripts accumulation of WRKY-a, WRKY1, POX (peroxidase), PR-1 (pathogenesis-related protein 1), and EAS (5-epiaristolochene synthase) was estimated by qRT-PCR, and they were compared with those recorded in the incompatible CM334-P. capsici (Pc) interaction. The levels of all studied genes were significantly (P ≤ 0.05) lower (WRKY1, POX and PR-1) or down-regulated (WRKY-a and EAS) in the presence of N. aberrans; in contrast, in the incompatible interaction, all genes were significantly up-regulated. The alterations induced by N. aberrans could be necessary to ensure the successful completion of its life cycle in CM334 chili roots.

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“…Mexico is considered the center of origin of C. annuum [ 12 ] and has a great genetic diversity within this species. Therefore, there is a high probability of finding chili pepper varieties resistant to pests and phytopathogens, such as chili ‘Criollo de Morelos’ (CM-334), which is resistant to Phytophthora capsici [ 13 ], to different phytopathogenic viruses [ 14 , 15 ], and to the nematodes Meloidogyne incognita , M . arenaria, and M .…”

Section: Introductionmentioning

confidence: 99%

Chili Pepper Jojutla Morelos (Capsicum annuum L.), CJ-2018: A Variety Resistant to Bactericera cockerelli

Silva-Valenzuela

Rojas-Martínez

Zúñiga-Mayo

2022

Insects

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Chili pepper is a vegetable of worldwide economic and gastronomic importance. The psyllid, Bactericera cockerelli, is an economically important pest in this crop, causing considerable losses in its production. Currently, the application of insecticides is the main way to control B. cockerelli. However, the use of varieties resistant to this insect is a viable alternative for its control and management. In this work, the oviposition rate, development, and survival of B. cockerelli in two native varieties of chili were evaluated. Choice and non-choice trials showed that the B. cockerelli oviposition was reduced on CJ-2018 by 92.17 and 80.18%, respectively, compared to the control. In CM-334, the insect showed a behavior similar to the control in the non-choice test, while in the choice test it laid more eggs on CM-334 compared to the control. The development and survival assay showed that only 1.33% of the eggs managed to reach the adult stage on CJ-2018. In contrast, on CM-334 the survival of B. cockerelli was similar to the control. These results suggest that CJ-2018 presented a resistance based on antixenosis and antibiosis against B. cockerelli.

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Mevalonate pathway genes expressed in chilli CM334 inoculated with Phytophthora capsici and infected by Nacobbus aberrans and Meloidogyne enterolobii

Cited by 7 publications

References 32 publications

Aptitud de cinco poblaciones de jitomate silvestre (Solanum lycopersicum var. cerasiforme) como hospedante del nematodo Nacobbus aberrans sensu lato

Aptitud de cinco poblaciones de jitomate silvestre (Solanum lycopersicum var. cerasiforme) como hospedante del nematodo Nacobbus aberrans sensu lato

Defense gene expression in root galls induced by Nacobbus aberrans in CM334 chilli plants

Chili Pepper Jojutla Morelos (Capsicum annuum L.), CJ-2018: A Variety Resistant to Bactericera cockerelli

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