Metschnikowia pulcherrima strain MACH1 outcompetes Botrytis cinerea, Alternaria alternata and Penicillium expansum in apples through iron depletion

Saravanakumar, Duraisamy; Ciavorella, Annalisa; Spadaro, D.; Garibaldi, A.; Gullino, M. L.

doi:10.1016/j.postharvbio.2007.11.006

Cited by 186 publications

(138 citation statements)

References 31 publications

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“…Our findings are similar to Harish et al (1998) who reported the reduced growth of Fusarium udum in the presence of extracellular proteins of the culture filtrate of Bacillus subtilis AF1. The yeast strain MACH1 showed higher efficacy against B. cinerea on apples and there are several examples of work performed in our centre on the biocontrol efficacy of M. pulcherrima strains against postharvest diseases of pome fruits (Piano et al 1997;Saravanakumar et al 2008). …”

Section: Discussionmentioning

confidence: 99%

“…Metschnikowia pulcherrima strain MACH1 (Saravanakumar et al 2008) and Rhodotorula sp. PW34 (Spadaro et al 2005) were isolated from the carposphere of apple cv.…”

Section: Yeast and Fungal Culturesmentioning

confidence: 99%

“…Competition for nutrients and space is believed to be the major mode of action of antagonistic yeasts (El-Ghaouth et al 1998;Spadaro et al 2002). Recently, we demonstrated that M. pulcherrima strain MACH1 could reduce the post-harvest pathogens of apple through competition for iron nutrient (Saravanakumar et al 2008). However, there is growing evidence to support the possible involvement of cell-wall degrading enzymes in the action of yeast antagonists.…”

Section: Introductionmentioning

confidence: 99%

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Detection of enzymatic activity and partial sequence of a chitinase gene in Metschnikowia pulcherrima strain MACH1 used as post-harvest biocontrol agent

et al. 2008

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Two antagonistic yeast strains Metschnikowia pulcherrima MACH1 and Rhodotorula sp. PW34 were tested for their efficacy against Botrytis cinerea in vitro and in vivo on apples. Metschnikowia pulcherrima strain MACH1 showed higher inhibition of B. cinerea compared to the strain PW34 in vitro on potato dextrose broth. Further, yeast strain MACH1 showed higher efficacy in reducing grey mould on apples compared to PW34 and the untreated control. In addition, partially purified extracellular proteins from strain MACH1 showed an inhibition to B. cinerea in vitro. The antagonistic yeast strains were tested for their efficacy to produce chitinases in different liquid media, including apple juice, amended with or without cell wall preparations (CWP) of B. cinerea. The study showed a higher production of chitinases from M. pulcherrima strain MACH1 when compared to PW34. Interestingly, the strain MACH1 secreted higher chitinases in the presence of cell wall fractions of B. cinerea. For this reason, the chitinase gene of strain MACH1 was amplified using PCR reactions and the nucleotide sequence data showed high homology to chitinases of other yeast strains.The results of the current study show that M. pulcherrima strain MACH1 has the ability to secrete chitinases in different liquid media including apple juice, and the enzyme could be involved in the postharvest biological control of B. cinerea.

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Section: Discussionmentioning

confidence: 99%

“…Metschnikowia pulcherrima strain MACH1 (Saravanakumar et al 2008) and Rhodotorula sp. PW34 (Spadaro et al 2005) were isolated from the carposphere of apple cv.…”

Section: Yeast and Fungal Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of enzymatic activity and partial sequence of a chitinase gene in Metschnikowia pulcherrima strain MACH1 used as post-harvest biocontrol agent

et al. 2008

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“…It has been demonstrated by several studies for antagonists such as Aureobasidium pullulans (Bencheqroun et al 2007), Cryptococcus humicola (Filonow et al 1996), Debaryomyces hansenii (Chalutz et al 1988), Metschnikowia pulcherrima (Saravanakumar et al 2008), P. agglomerans (Poppe et al 2003) or Rhodotorula glutinis (Castoria et al 1997).…”

Section: Mechanisms Of Action Of Postharvest Biological Control Agentsmentioning

confidence: 98%

Biological control of postharvest diseases of fruit

2011

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Postharvest decay in harvested fruit causes considerable economical losses. Fungicides are the primary means to control these losses. Public concern in food safety and environmental issues and the increase of pathogen resistant populations have enhanced the interest in developing alternative methods to fungicides to control postharvest fruit decay. During the last two decades a huge information and advances concerning the selection of antagonists, mode of action, different approaches to enhance biocontrol activity, formulation and production have been achieved, and some biofungicides are already in the market. It is likely that several more products will enter the market in the near future, as the result of the biological control research programs worldwide. Nonetheless, it is necessary to continue finding new potential microorganisms, better understanding the mode of action, and pathogen, antagonist and host interactions, to increase the potential of biocontrol helping to become a real alternative to synthetic postharvest fungicides. This article presents an overview of postharvest biological control approaches and explores new research possibilities to improve biocontrol activity.

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“…Khamir memiliki mekanisme antagonisme berupa kompetisi nutrisi dan ruang, parasitisme dan produksi enzim litik serta induksi ketahanan sehingga mampu mengendalikan beberapa patogen pascapanen (Nunes 2012). Metschnikowia pulcherrima memiliki kemampuan kompetisi besi untuk menghambat Botrytis cinerea, Alternaria alternata dan Penicillium expansum pada apel (Saravanakumar et al 2008). Pichia membranefaciens dan C. albidus memiliki kemampuan untuk melekat pada hifa Monilinia fructicola, P. expansum, dan Rhizopus stolonifer (Chan & Tian 2005).…”

Section: Seleksi Antagonisme Isolat Khamir Epifit Terhadap Antraknosaunclassified

Seleksi Khamir Epifit Sebagai Agens Antagonis Penyakit Antraknosa Pada Cabai

Hartati

Wiyono

Hidayat

et al. 2016

J.Hort

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Naskah diterima tanggal 21 Agustus 2014 dan disetujui untuk diterbitkan tanggal 26 September 2014 ABSTRAK. Antraknosa merupakan penyakit penting pada tanaman cabai yang dapat menyebabkan kerugian ekonomi cukup besar. Khamir merupakan salah satu mikroba yang telah diketahui berpotensi sebagai agens antagonis pada berbagai produk pascapanen. Penelitian ini bertujuan untuk mendapatkan isolat-isolat khamir epifit yang berpotensi sebagai agens antagonis penyakit antraknosa pada cabai. Penelitian dilaksanakan di Laboratorium Mikologi Tumbuhan dan Kebun Percobaan Institut Pertanian Bogor, dari bulan April sampai Desember 2013. Khamir epifit diisolasi dari daun dan buah cabai merah yang diperoleh dari pertanaman cabai di Rancabango dan Panjiwangi (Kabupaten Garut) dan Dramaga (Kabupaten Bogor). Patogen penyebab antraknosa yaitu Colletotrichum acutatum diisolasi dari buah cabai bergejala dari pertanaman cabai di Panjiwangi. Khamir hasil isolasi diuji patogenisitasnya pada benih dan buah cabai. Khamir nonpatogenik diseleksi potensi antagonismenya terhadap penyebab penyakit antraknosa. Diperoleh 43 isolat khamir epifit, semua isolat bersifat nonpatogenik berdasarkan hasil uji patogenisitas. Seleksi potensi antagonisme isolat khamir epifit menghasilkan 23 isolat yang berpotensi sebagai agens antagonis C. acutatum. Empat belas isolat khamir epifit menyebabkan penghambatan penyakit antraknosa lebih besar dibandingkan mankozeb. Katakunci: Colletotrichum acutatum; Capsicum annuum; FungisidaABSTRACT. Anthracnose is a major disease on chili which economically causes a great loss. Yeast was proven as potential antagonistic agents against various post harvest pathogens. This research was aimed to obtain epiphytic yeast isolates which is potential as antagonistic agents against anthracnose on chili. The experiments were carried out in the Laboratory of Plant Mycology and University Research Farm Field at Bogor Agricultural University from April to December 2013. The epiphytic yeasts were isolated from leaves and fruits of red chili grown at Rancabango and Panjiwangi (Garut) and Dramaga (Bogor). Anthracnose pathogen, Colletotrichum acutatum, was isolated from diseased chili fruit grown at Panjiwangi. The pathogenicity of the yeast isolates was tested on the seed and fruit of chili. Nonpathogenic yeasts were selected for the antagonistic potential against anthracnose pathogen. Forty three isolates of epiphytic yeasts was obtained, all of them was nonpathogenic based on pathogenicity test. Twenty three epiphytic isolates had the antagonistic potential against C. acutatum. There were 14 epiphytic yeast isolates that caused greater inhibition of anthracnose disease than mancozeb.

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Metschnikowia pulcherrima strain MACH1 outcompetes Botrytis cinerea, Alternaria alternata and Penicillium expansum in apples through iron depletion

Cited by 186 publications

References 31 publications

Detection of enzymatic activity and partial sequence of a chitinase gene in Metschnikowia pulcherrima strain MACH1 used as post-harvest biocontrol agent

Detection of enzymatic activity and partial sequence of a chitinase gene in Metschnikowia pulcherrima strain MACH1 used as post-harvest biocontrol agent

Biological control of postharvest diseases of fruit

Seleksi Khamir Epifit Sebagai Agens Antagonis Penyakit Antraknosa Pada Cabai

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