Metrology of cellular analysis: problems and solutions

Abstract

Cellular analysis is one of the perspective developing areas of metrology. The complexity of the measuring object requires novel approaches and correct metrological support for the quantitative methods of cellular analysis. Definition of the quantitative parameters and nominal properties of the cell, standardization of methodology – these are main tasks of the Cellular Analysis Working Group of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology. The results of a number of pilot studies carried out by Working Group can be used as a first step establishing metrological traceability of the cell measurements. Development of the stable reference materials for cell quantification is a challenging problem. One of the promising solutions seems to apply synthetic cell-mimicking particles. Currently we are in the process of developing candidate synthetic reference materials for cell measurements.

Одержання Та Опис Екзометаболітів Мезенхімальных Стовбурових Клітин З Кісткового Мозку Собаки

“…×100, Biolab-I). The proliferative index was calculated as the percentage of cells on the 7th day of growth to the seed concentration; cells were counted in a Goryaev hemocytometer; dead cells sub-vitally stained with trypan blue were not taken into account [23]. A conditioned medium containing MSCs exometabolites was sampled after 48 h of growth of the corresponding passage.…”