Metodologias analíticas para determinação das fumonisinas em milho e alimentos à base de milho

Lino, Celeste M.; Silva, Liliana J.G.; Pena, Angelina

doi:10.1590/s0100-40422006000200021

Cited by 9 publications

(8 citation statements)

References 45 publications

(113 reference statements)

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“…Monitoring the presence of these mycotoxins is crucial in view of their relevance. The classical methods of mycotoxins determination involve solvent toxin extraction processes and detection by chromatographic methods [ 48 ]. These techniques are very accurate, but time-consuming and costly, thus hampering analyses of large samples and real-time decision making [ 49 , 50 ].…”

Section: Introductionmentioning

confidence: 99%

Multivariate method for prediction of fumonisins B1 and B2 and zearalenone in Brazilian maize using Near Infrared Spectroscopy (NIR)

Tyska

Mallmann²,

Vidal

et al. 2021

PLoS ONE

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Fumonisins (FBs) and zearalenone (ZEN) are mycotoxins which occur naturally in grains and cereals, especially maize, causing negative effects on animals and humans. Along with the need for constant monitoring, there is a growing demand for rapid, non-destructive methods. Among these, Near Infrared Spectroscopy (NIR) has made great headway for being an easy-to-use technology. NIR was applied in the present research to quantify the contamination level of total FBs, i.e., fumonisin B1+fumonisin B2 (FB1+FB2), and ZEN in Brazilian maize. From a total of six hundred and seventy-six samples, 236 were analyzed for FBs and 440 for ZEN. Three regression models were defined: one with 18 principal components (PCs) for FB1, one with 10 PCs for FB2, and one with 7 PCs for ZEN. Partial least square regression algorithm with full cross-validation was applied as internal validation. External validation was performed with 200 unknown samples (100 for FBs and 100 for ZEN). Correlation coefficient (R), determination coefficient (R2), root mean square error of prediction (RMSEP), standard error of prediction (SEP) and residual prediction deviation (RPD) for FBs and ZEN were, respectively: 0.809 and 0.991; 0.899 and 0.984; 659 and 69.4; 682 and 69.8; and 3.33 and 2.71. No significant difference was observed between predicted values using NIR and reference values obtained by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS), thus indicating the suitability of NIR to rapidly analyze a large numbers of maize samples for FBs and ZEN contamination. The external validation confirmed a fair potential of the model in predicting FB1+FB2 and ZEN concentration. This is the first study providing scientific knowledge on the determination of FBs and ZEN in Brazilian maize samples using NIR, which is confirmed as a reliable alternative methodology for the analysis of such toxins.

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Section: Introductionmentioning

confidence: 99%

Multivariate method for prediction of fumonisins B1 and B2 and zearalenone in Brazilian maize using Near Infrared Spectroscopy (NIR)

Tyska

Mallmann²,

Vidal

et al. 2021

PLoS ONE

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“…Recently, Lino et al [15] reviewed the analytical methodology available for the determination of fumonisins in foods. These methods generally involve liquid extraction, with mixtures of polar solvents, such as methanol/ water [16,17], acetonitrile/water [18], methanol/acetonitrile/water [19], and acetonitrile/NaH 2 PO 4 [20].…”

Section: Introductionmentioning

confidence: 99%

“…Different derivatization reagents have been reported, namely, o-phthaldialdehyde (OPA)-the most used [8,9,21], naphthalene-2,3-dicarboxaldehyde (NDA) [19,20,23], and 4-fluoro-7-nitrobenzofurazan (NBD-F) [24]. Detection and quantification have preferentially been performed using HPLC with fluorescence or mass spectrometry detection [8,9,21,22,[25][26][27][28] although other chromatographic, electrophoretic, and immunological methods have been reported [15].…”

Section: Introductionmentioning

confidence: 99%

Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection

et al. 2006

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Fumonisins B 1 (FB 1 ) and fumonisin B 2 (FB 2 ) are the main members of a family of mycotoxins produced by Fusarium verticillioides, Fusarium proliferatum, and other fungi species of the section Liseola. The present work shows the results of comparative studies using two different procedures for the analysis of fumonisins in maize and maize-based samples. The studied analytical methods involve extraction with methanol/water, dilution with PBS, and clean-up through immunoaffinity columns. Two reagents (o-phthaldialdehyde and naphthalene-2,3-dicarboxaldehyde) were studied for formation of fluorescent derivatives. The separation and identification were carried out by high-performance liquid chromatography with fluorescence detection. The optimized method for analysis of fumonisins in maize involved extraction with methanol/ water (80:20), clean-up with an immunoaffinity column, and derivatization with naphthalene-2,3-dicarboxaldehyde (NDA). The limit of detection was 20 μg kg −1 for FB 1 and 15 μg kg −1 for FB 2 . Recoveries of FB 1 and FB 2 ranged from 79% to 99.6% for maize fortified at 150 μg kg −1 and 200 μg kg −1 , respectively, with within-day RSDs of 3.0 and 2.7%. The proposed method was applied to 31 samples, and the presence of fumonisins was found in 14 samples at concentrations ranging from 113 to 2,026 μg kg −1 . The estimated daily intake of fumonisins was 0.14 μg kg −1 body weight per day.

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“…Métodos de análise são essenciais para determinação do grau de contaminação por micotoxinas, avaliação de exposição ao risco, confirmação do diagnóstico de micotoxicoses, assim como para monitorar e indicar estratégias para diminuição destes agentes tóxicos presentes nos alimentos (Lino et al, 2006, Binder, 2007, Bryden, 2012, Mallmann et al, 2013. Estes métodos de análise devem preferencialmente ser simples, rápidos, sensíveis, seletivos, confiáveis e de baixo custo (Stroka et al, 2004, Amaral and Machinski Junior, 2006, Scussel et al, 2011.…”

Section: Metodologias Analíticas Para Detecção E Quantificação De Micunclassified

Procedimentos para colheita de amostras e análises laboratoriais de micotoxinas presentes em dietas humanas e animais

Savi¹,

Pereira

Frozza³

et al. 2017

Pubvet

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RESUMO.Micotoxinas são substâncias tóxicas produzidas pelo metabolismo secundário de fungos filamentosos e capazes de desencadear uma série de problemas relacionados à produção agropecuária e à saúde pública. O monitoramento destes agentes tóxicos é necessário, devendo-se levar em consideração para realização de tal atividade a diversidade das amostras que são colhidas, os tipos distintos de micotoxinas, bem como a distribuição não uniforme destes contaminantes. A escolha adequada dos pontos de amostragem, bem como do uso de uma metodologia eficaz que considere os possíveis interferentes e contemple os níveis de detecção esperados também são fatores indispensáveis para o sucesso e confiabilidade das análises realizadas. Com o intuito de atender às exigências da legislação brasileira para micotoxinas, vários alimentos deverão ser analisados, para isso, o conhecimento de procedimentos para colheita de amostras e análises laboratoriais são importantes para pequenos produtores e para indústria de alimentos. Considerando que a aplicação adequada destas metodologias não apenas pode aumentar a produção agrícola e sua consequente lucratividade, como também propiciar a segurança alimentar dos diversos produtos brasileiros destinados às dietas humanas e/ou animais, esta revisão visa descrever as principais metodologias existentes no Brasil para colheita de amostras e análise de micotoxinas, assim como abordar os principais métodos analíticos existentes para detecção e quantificação destes contaminantes. Palavras chave: Alimentos, amostragem, análises laboratoriais, metodologias analíticasProcedures for sampling and laboratory tests of mycotoxins existing in human and animals feed ABSTRACT. Mycotoxins are toxic substances produced by the secondary metabolism of filamentous fungi and able to trigger series of problems related to agricultural production and public health. Thereby, monitoring of toxic agents is absolutely necessary and must be taken into account for the performance of such activity the diversity of samples to be collected, the different types of mycotoxins as well as the non-uniform distribution of these contaminants. Proper selection of sampling process as well as the use of a effective methodology will eliminate the trace of interference in order to have a successful analyses. Thus, in order to meet the requirements of the current Brazilian legislation for mycotoxins, various foods should be analyzed for this, knowledge of procedures for sampling and laboratory analyzes are of fundamental importance to both small producers and for the food

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Metodologias analíticas para determinação das fumonisinas em milho e alimentos à base de milho

Cited by 9 publications

References 45 publications

Multivariate method for prediction of fumonisins B1 and B2 and zearalenone in Brazilian maize using Near Infrared Spectroscopy (NIR)

Multivariate method for prediction of fumonisins B1 and B2 and zearalenone in Brazilian maize using Near Infrared Spectroscopy (NIR)

Determination of fumonisins B1 and B2 in Portuguese maize and maize-based samples by HPLC with fluorescence detection

Procedimentos para colheita de amostras e análises laboratoriais de micotoxinas presentes em dietas humanas e animais

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