Methylsulfonylmethane exhibits bacteriostatic inhibition of Escherichia coli , and Salmonella enterica Kinshasa, in vitro

Abstract

Aims To evaluate antibacterial properties of methylsulfonylmethane (MSM) on Escherichia coli (MDRE21) and Salmonella enterica serovar Kinshasa (SK132). Methods and Results Bacterial proliferation analysis was measured spectrophotometrically during log phase growth with 0, 3, 5, 7, 10, 12 and 16% MSM. To assess the mechanism of inhibition, cultures were grown overnight with 0–16% MSM and enumerated on unmedicated brain–heart infusion agar (BHIA) or BHIA with 0–16% MSM. The long‐term viability studies were done to evaluate the impact of 10% MSM. Absorbance data indicated a dose‐dependent inhibition from 0 to 16% MSM. There was no growth of MDRE21 or SK132 on BHIA in 10–16% MSM. Both strains enumerated on unmedicated BHIA from overnight cultures with 10–16% MSM were able to partially recover. Conclusions Recovery after MSM removal may be indicative of a bacteriostatic mechanism of inhibition. The long‐term viability studies illustrated that neither MDRE21 nor SK132 could be rescued from 10% MSM after 5 or 6 days respectively. Significance and Impact of the Study Methylsulfonylmethane antibacterial activity may prove useful during pre or postharvest food safety as a disinfectant. The primary benefit being, its clinical safety to humans. Comparisons to other disinfectants would also need to be done to determine if MSM was superior to those already on the market and would be cost effective.

Effects of dietary methyl sulfonyl methane and selenium on laying performance, egg quality, gut health indicators, and antioxidant capacity of laying hens

“…However, a clear explanation of the MSM-mediated improvement in ileal morphology is not available. In earlier studies, MSM was shown to exhibit antimicrobial activity or immunemodulating activity [27,35]. However, the finding that dietary MSM did not affect ileal SCFAs in this study suggests that the antimicrobial activity of MSM might not be responsible for MSM-mediated improvement in ileal morphology.…”

Incorporation of Dietary Methyl Sulfonyl Methane into the Egg Albumens of Laying Hens

“…It has been well reported that dietary MSM plays an important role in reducing oxidative stress [12,15], inhibiting pathogenic bacteria [30], and improving the host's immune response [31]. In addition, MSM is known to inhibit the cortisol-induced stress response in horse skeletal muscle cells.…”

Effects of Dietary Organic and Inorganic Sulfur on Laying Performance, Egg Quality, Ileal Morphology, and Antioxidant Capacity in Laying Hens

“…The structure and functionality of the intestinal microbiota are crucial for the health of poultry. Due to the antimicrobial activities of MSM [ 43 ] and SS [ 44 ], it is expected that their supplementation in the diets of laying hens could balance gut microbiota and improve gut health, which prompted us to measure ileal morphology and ileal SCFAs. Both MSM and SS did not affect ileal SCFAs, but increased villus height and crypt depth ratio, the indicator of gut function and health [ 45 , 46 ].…”