Methylobacterium-Induced Endophyte Community Changes Correspond with Protection of Plants against Pathogen Attack

Ardanov, Pavlo; Sessitsch, Angela; Häggman, Hely; Kozyrovska, Natalia; Pirttilä, Anna Maria

doi:10.1371/journal.pone.0046802

Cited by 116 publications

(87 citation statements)

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“…Since niche similarities can adversely impact coexistence (41), there may have been resource-related competition between the pathogen and other members of its bacterial class. Previous studies have also indicated Methylobacterium and Sphingomonas to be involved in protecting host plants from various pathogens (42)(43)(44)(45). Furthermore, by examining the year-long cooccurrence patterns for the 20 most abundant families and Liberibacter spp., we uncovered negative correlations between the pathogen and three bacterial families (Burkholderiaceae, Xanthomonadaceae, and Micromonosporaceae) that have previously been described to have plant-beneficial properties (18, 23, 27, 46).…”

Section: Discussionmentioning

confidence: 99%

Defining the Core Citrus Leaf- and Root-Associated Microbiota: Factors Associated with Community Structure and Implications for Managing Huanglongbing (Citrus Greening) Disease

Blaustein

Lorca

Meyer

et al. 2017

Appl Environ Microbiol

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Stable associations between plants and microbes are critical to promoting host health and productivity. The objective of this work was to test the hypothesis that restructuring of the core microbiota may be associated with the progression of huanglongbing (HLB), the devastating citrus disease caused by Liberibacter asiaticus, Liberibacter americanus, and Liberibacter africanus. The microbial communities of leaves (n ϭ 94) and roots (n ϭ 79) from citrus trees that varied by HLB symptom severity, cultivar, location, and season/time were characterized with Illumina sequencing of 16S rRNA genes. The taxonomically rich communities contained abundant core members (i.e., detected in at least 95% of the respective leaf or root samples), some overrepresented site-specific members, and a diverse community of lowabundance variable taxa. The composition and diversity of the leaf and root microbiota were strongly associated with HLB symptom severity and location; there was also an association with host cultivar. The relative abundance of Liberibacter spp. among leaf microbiota positively correlated with HLB symptom severity and negatively correlated with alpha diversity, suggesting that community diversity decreases as symptoms progress. Network analysis of the microbial community time series identified a mutually exclusive relationship between Liberibacter spp. and members of the Burkholderiaceae, Micromonosporaceae, and Xanthomonadaceae. This work confirmed several previously described plant disease-associated bacteria, as well as identified new potential implications for biological control. Our findings advance the understanding of (i) plant microbiota selection across multiple variables and (ii) changes in (core) community structure that may be a precondition to disease establishment and/or may be associated with symptom progression.IMPORTANCE This study provides a comprehensive overview of the core microbial community within the microbiomes of plant hosts that vary in extent of disease symptom progression. With 16S Illumina sequencing analyses, we not only confirmed previously described bacterial associations with plant health (e.g., potentially beneficial bacteria) but also identified new associations and potential interactions between certain bacteria and an economically important phytopathogen. The importance of core taxa within broader plant-associated microbial communities is discussed.KEYWORDS core microbiome, plant-associated microbiota, Liberibacter, pathogenmicrobe interactions

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Section: Discussionmentioning

confidence: 99%

Defining the Core Citrus Leaf- and Root-Associated Microbiota: Factors Associated with Community Structure and Implications for Managing Huanglongbing (Citrus Greening) Disease

Blaustein

Lorca

Meyer

et al. 2017

Appl Environ Microbiol

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“…Endophytes establish a mutualistic symbiosis with their hosts because they are selectively favoured 3,4 . They are classified according to their colonization strategy.…”

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“…Facultative endophytes live in the soil and are able to colonize the plant when the opportunity arises. Passive endophytes colonize the plant through open wounds in the root hairs 3,5 . Endophyte community structure and composition are closely related to plant phenology, soil type, pH and bacterial density in the rhizosphere 4 .…”

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“…Endophytes stimulate plant growth through several mechanisms such as nitrogen fixation, inorganic phosphate solubilization, siderophore production, micronutrient supply 6 , photosynthetic activity and phytostimulation 5 . Endophytic bacteria can also contribute to soil pathogen control with the production of antibiotics and secondary metabolites and induction of the plant defence system 3 , besides heavymetal biotransformation, biodegradation of organic pollutants 2 , osmotic adjustment, stomatal regulation, modification of the root morphology and metabolism 1 . Jojoba (Simmondsia chinensis) is a shrub native to northwestern Mexico and southwestern United States.…”

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Isolation and Characterization of Endophytic Bacteria Associated with Roots of Jojoba (<i>Simmondsia chinensis</i> (Link) Schneid)

Elvia¹,

Lilia²,

Puente³

et al. 2017

Current Science

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In this communication, the diversity and beneficial characteristics of endophytic bacteria have been studied in Simmondsia chinensis that has industrial importance because of the quality of its seed oil. Endophytes were isolated (N = 101) from roots of the jojoba plants collected, of which eight were identified by partial sequencing of the 16S rDNA gene. The isolated bacteria were Bacillus sp., Methylobacterium aminovorans, Oceanobacillus kimchi, Rhodococcus pyridinivorans and Streptomyces sp. All isolates had at least one positive feature, characterizing them as potential plant growth promoting bacteria. In this study, R. pyridinivorans and O. kimchi are reported as plant growth promoters.

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“…Under necessity, for example, under some external threat, the endophytic bacterial community can be mobilized by some external stimulus and rise a size of their populations [28,29,35]. Activating endophytic residents coincided with the better survival of potato vitroplants under post vitro conditions and this may serve as an in- The maximum quantum yield (Fv/Fm), the efficient quantum yield (Ô PSII ), the PSII maximum efficiency (Fv'/Fm'), the photochemical quenching direct preliminary evidence that resident endophytes invest in the immune response [36,37]. We can postulate that resident endophytic bacterial communities (as well as other endophytic microorganisms) have a potency to modulate the plant protection system against external threat.…”

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2013

Biopolym. Cell

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Bacteria are micro-and nanoorganisms that spatially colonize the plant organism and comprise various types of interactions with the host ranging fromIntroduction. Plants need energy to drive photosynthesis which converts light energy to chemical energy in the form of energy-rich carbohydrates. Photosynthesis is regulated by environmental factors as well as endogenous sugar signals. Plant-associated bacteria consume plant-derived photosynthates, and this is why steering the photosystem.A rate of photosynthesis is usually evaluated by measuring chlorophyll (Chl) a fluorescence. In intact leaves at room temperature, the majority of Chl a fluorescence arises from photosystem II (PSII). The fluorescence yield of PSII is variable as a result of competition between photochemical and non-photochemical processes, and the outcome of this interaction changes Chl fluorescence yield. Chl a fluorescence measurement has been proven to be a useful non-invasive tool for the study of different aspects of photosynthesis, and for the quantification of any stressor impact in plants [1]. Interaction of

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Methylobacterium-Induced Endophyte Community Changes Correspond with Protection of Plants against Pathogen Attack

Cited by 116 publications

References 54 publications

Defining the Core Citrus Leaf- and Root-Associated Microbiota: Factors Associated with Community Structure and Implications for Managing Huanglongbing (Citrus Greening) Disease

Defining the Core Citrus Leaf- and Root-Associated Microbiota: Factors Associated with Community Structure and Implications for Managing Huanglongbing (Citrus Greening) Disease

Isolation and Characterization of Endophytic Bacteria Associated with Roots of Jojoba (<i>Simmondsia chinensis</i> (Link) Schneid)

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