Isolation and Characterization of Endophytic Bacteria Associated with Roots of Jojoba (<i>Simmondsia chinensis</i> (Link) Schneid)

Abstract

In this communication, the diversity and beneficial characteristics of endophytic bacteria have been studied in Simmondsia chinensis that has industrial importance because of the quality of its seed oil. Endophytes were isolated (N = 101) from roots of the jojoba plants collected, of which eight were identified by partial sequencing of the 16S rDNA gene. The isolated bacteria were Bacillus sp., Methylobacterium aminovorans, Oceanobacillus kimchi, Rhodococcus pyridinivorans and Streptomyces sp. All isolates had at least one positive feature, characterizing them as potential plant growth promoting bacteria. In this study, R. pyridinivorans and O. kimchi are reported as plant growth promoters.

Surface sterilization for isolation of endophytes: Ensuring what (not) to grow

“…By and large, in the ethanol-sodium hypochlorite combination, the concentration and exposure of ethanol are constant. Around 70%-75% ethanol is used for 0.5-1 min [37,92]. However, there are a few reports of 95%-99% ethanol being used as sterilants [46,117].…”

Studies on Endophytic Actinobacteria as Plant Growth Promoters and Biocontrol Agents

“…showed antifungal activity against Geotrichum candidum, F. oxysporum, Alternaria sp. [142]. According to Passari et al [137], the growth of various phytopathogens including Fusarium Oxysporum, Fusarium graminearum, Rhizoctonia solani, Colletotrichum capsici were inhibited by endophytic actinobacteria such as Nocardiopsis sp., Streptomyces sp.…”