Methylmercury in tuna: demonstrating measurement capabilities and evaluating comparability of results worldwide from the CCQM P-39 comparison

Quétel, Christophe R.; Snell, James; Yetunde, Aregbe; Abrankó, László; Jókai, Zsuzsa; Brunori, Claudia; Morabito, Roberto; Hagan, W.; Azemard, Sabine; Wyse, E.; Fajon, Vesna; Horvat, Milena; Logar, M.; Donard, Olivier F. X.; Krupp, Eva M.; Entwisle, John; Hearn, Ruth; Schantz, Michele M.; Inagaki, Kenji; Takatsu, Akiko; Grinberg, Patrícia; Willie, Scott; Dimock, Brian; Hintelmann, Holger; Zhu, Joy; González, Elisa Blanco; Centineo, Giuseppe; Alonso, J. Ignacio García; Sanz‐Medel, Alfredo; Björn, Erik

doi:10.1039/b505368d

Cited by 11 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9). 182,183 An excellent overall result has been obtained within this study. An overall spread of 14% has been achieved despite the fact that different extraction/preparation protocols have been applied.…”

Section: Speciation Analysissupporting

confidence: 63%

Characterisation of reference materials by isotope dilution mass spectrometry

Vogl

2007

J. Anal. At. Spectrom.

View full text Add to dashboard Cite

Section: Speciation Analysissupporting

confidence: 63%

Characterisation of reference materials by isotope dilution mass spectrometry

Vogl

2007

J. Anal. At. Spectrom.

View full text Add to dashboard Cite

“…Another approach is to overcome matrix effects with species-specific isotope dilution followed by species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICP-MS). SSID-GC-ICP-MS has often been used as the gold standard for methylmercury determination in a complex matrix such as biological tissues [17][18][19]. Hence, not only expensive isotopically enriched mercury as Me 201 Hg is needed but also the hyphenated technique of GC-ICP-MS.…”

Section: Introductionmentioning

confidence: 99%

Direct online HPLC-CV-AFS method for traces of methylmercury without derivatisation: a matrix-independent method for urine, sediment and biological tissue samples

Brombach

Gajdosechova

Chen³

et al. 2014

Anal Bioanal Chem

View full text Add to dashboard Cite

Mercury (Hg) is a global pollutant which occurs in different species, with methylmercury (MeHg) being the critical compound due to its neurotoxicity and bioaccumulation through the food chain. Methods for trace speciation of MeHg are therefore needed for a vast range of sample matrices, such as biological tissues, fluids, soils or sediments. We have previously developed an ultra-trace speciation method for methylmercury in water, based on a preconcentration HPLC cold vapour atomic fluorescence spectrometry (HPLC-CV-AFS) method. The focus of this work is mercury speciation in a variety of sample matrices to assess the versatility of the method. Certified reference materials were used where possible, and samples were spiked where reference materials were not available, e.g. human urine. Solid samples were submitted for commonly used digestion or extraction processes to obtain a liquid sample for injection into the analytical system. For MeHg in sediment samples, an extraction procedure was adapted to accommodate MeHg separation from high amounts of Hg(2+) to avoid an overload of the column. The recovery for MeHg determination was found to be in the range of 88-104% in fish reference materials (DOLT-2, DOLT-4, DORM-3), lobster (TORT-2), seaweed (IAEA-140/TM), sediments (ERM(®)-CC580) and spiked urine and has been proven to be robust, reliable, virtually matrix-independent and relatively cost-effective. Applications in the ultra-trace concentration range are possible using the preconcentration up to 200 mL, while for higher MeHg-containing samples, lower volumes can be applied. A comparison was carried out between species-specific isotope dilution gas chromatography inductively coupled plasma mass spectrometry (SSID-GC-ICP-MS) as the gold standard and HPLC-CV-AFS for biological tissues (liver, kidney and muscle of pilot whales), showing a slope of 1.008 and R (2) = 0.97, which indicates that the HPLC-CV-AFS method achieves well-correlated results for MeHg in biological tissues.

show abstract

“…2 Methylmercury can then be accumulated and amplified along the marine food chain. 3 The development of speciated isotope dilution (SID) procedures has drastically improved the accuracy of mercury speciation measurements, 4 although most studies have been focused on MeHg. The isotopic tracer capabilities of this technique have also revealed that mercury species are subject to artificial transformations [5][6][7][8] (i.e., methylation and demethylation) during analyses of environmental matrices, such as seawater, 9,10 sediments 7,[11][12][13] and biological materials.…”

Section: Introductionmentioning

confidence: 99%

Consideration and influence of complexed forms of mercury species on the reactivity patterns determined by speciated isotope dilution model approaches: A case for natural biological reference materials

Point

Alonso

Davis

et al. 2008

J. Anal. At. Spectrom.

Self Cite

View full text Add to dashboard Cite

The processes driving the inadvertent transformations of inorganic mercury (iHg) and methylmercury (MeHg) in cryogenically homogenized fresh-frozen (FF) and freeze-dried (FD) biological standard reference materials (SRM) were investigated using alkaline digestion, derivatization and gas chromatography inductively coupled plasma mass spectrometry (GC-ICP-MS). Labile inorganic mercury ( 201 iHg) and methylmercury (Me 202 Hg) isotopic standards and their cysteine-complexed analogs ( 201 Hg(Cys) 2 and Me 202 HgCys) were used in a double-spike speciated isotope dilution (SID) model to document the influence of complexing ligands on the equilibration, the reactivity and the transformation processes between isotopic mercury species and their endogenous analogs. Cysteinecomplexed and labile Me 202 Hg spiking standards displayed similar equilibration processes in both classes of materials, leading to accurate MeHg determinations with negligible methylation transformations. Labile 201 iHg standards provided accurate iHg concentration results in both material series, although an apparent demethylation reaction specific of FF materials was detected, with a negligible effect in FD materials. Cysteine-complexed 201 iHg standards led to higher but inaccurate iHg concentrations and higher demethylation yields in both materials. This comparison illustrated a significant influence of complexing ligands on the equilibration processes between labile and/or complexed 201 iHg species and their endogenous analogs. The derivatization step was found to catalyze these non-equilibrium conditions with different derivatization yields between labile and complexed iHg species, whereas no differences were observed for MeHg species. When complexation disparities existed in solution between 201 iHg species and their endogenous analogs, this process specifically affected the determination of 200/201 iHg ratios relative to 200/202 iHg, 200/201 MeHg and 200/202 iHg ratios, which are all involved in the double-spike SID model to establish transformation yields and mercury species concentrations. This process and the influence of demethylating agents were presumably responsible for the apparent demethylation reaction only observed in FF materials. This result gave rise to several questions on the influence of freeze-drying procedures on the lability/complexation patterns of mercury species and/or on the activity of demethylating agents. A complementary analytical step consisting of maximizing the equilibrium conditions between isotopic and endogenous mercury species together with inhibiting the activity of demethylating agent is proposed. When applying these conditions, the two classes of materials displayed negligible transformation reactions, improving their commutability for the simultaneous determination of iHg and MeHg. The approach proposed in this work allowed for the first time documentation of the potential and limitations of speciated isotope dilution procedures to handle the problem of lability/complexation and ligand interactions in natur...

show abstract

Methylmercury in tuna: demonstrating measurement capabilities and evaluating comparability of results worldwide from the CCQM P-39 comparison

Cited by 11 publications

References 33 publications

Characterisation of reference materials by isotope dilution mass spectrometry

Characterisation of reference materials by isotope dilution mass spectrometry

Direct online HPLC-CV-AFS method for traces of methylmercury without derivatisation: a matrix-independent method for urine, sediment and biological tissue samples

Consideration and influence of complexed forms of mercury species on the reactivity patterns determined by speciated isotope dilution model approaches: A case for natural biological reference materials

Contact Info

Product

Resources

About