Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione

Yadav, Sudesh Kumar; Singla‐Pareek, Sneh L.; Ray, Manju; Reddy, M. K.; Sopory, Sudhir K.

doi:10.1016/j.bbrc.2005.08.263

Cited by 404 publications

(353 citation statements)

References 27 publications

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“…Methylglyoxal, besides its own cytotoxicity, lowers the level of glutathione, by the formation of hemithioactal with glutathione. Under stress conditions, the increased level of methylglyoxal could further lower the glutathione level required for scavenging of reactive oxygen species, which increase under stress conditions [25]. Since the glyoxalase I has been shown to be upregulated under different abiotic stresses [14,15] and an increase in the glyoxalase II transcript has been observed under various abiotic stresses in Brasssica juncea [17] and in rice [18], the overexpression of the enzymes of the glyoxalase system is presumed to be involved in stress tolerance by detoxifying methylglyoxal and elevating the level of glutathione.…”

Section: Resultsmentioning

confidence: 99%

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

Saxena¹

2011

TOPSJ

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Engineering of salinity tolerance in agronomically important crop plants is required to increase their productivity by enabling them to grow in saline soils, which are otherwise left uncultivated. Since an increase in the enzymes of glyoxalase system has been shown to impart salinity tolerance in the model plant tobacco, we used the glyoxalase II gene for engineering salinity tolerance in an important oil yielding crop, Brassica juncea. The transgenic plants of B. juncea overexpressing the glyoxalase II gene showed higher salinity tolerance as compared to the untransformed control plants as observed by delayed senescence in leaf discs at 400 mM and 800 mM NaCl in T1 generation. The percentage of germination of the T2 transgenic seeds was higher at 150 mM and 200 mM NaCl as compared to the seeds of untransformed plants. This for the first time demonstrates the applicability of utilizing the glyoxalase II gene for enhanced salinity tolerance in an oilseed crop plant B. juncea.

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Section: Resultsmentioning

confidence: 99%

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

Saxena¹

2011

TOPSJ

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“…Besides acting as a signaling molecule, 22) MG in cells can cause oxidative damage to cellular constituents. 21,23) In the present study, both short-term and long-term NaCl treatment induced MG accumulation in BY-2 cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…[12][13][14][18][19][20] In plant cells, salinity also leads to the accumulation of methylglyoxal (MG), a cytotoxic compound that can react with and modify other molecules, including DNA and proteins. [21][22][23] Because of the highly cytotoxic and reactive properties of H 2 O 2 , O 2 À , NO, and MG, their concentrations must be kept under strict control. Plants possess antioxidant defense and glyoxalase systems to protect their cells against the damaging effects of H 2 O 2 , O 2 À , and MG. 13,19,22,23) Proline, besides functioning as an osmoprotectant, suppresses production of free radicals 1,6,11) and reactive oxygen species (ROS).…”

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confidence: 99%

“…[21][22][23] Because of the highly cytotoxic and reactive properties of H 2 O 2 , O 2 À , NO, and MG, their concentrations must be kept under strict control. Plants possess antioxidant defense and glyoxalase systems to protect their cells against the damaging effects of H 2 O 2 , O 2 À , and MG. 13,19,22,23) Proline, besides functioning as an osmoprotectant, suppresses production of free radicals 1,6,11) and reactive oxygen species (ROS). 24,25) Our recent study indicated that both exogenous proline and betaine decrease H 2 O 2 accumulation induced by NaCl 12) and cadmium.…”

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Proline and Glycinebetaine Ameliorated NaCl Stress via Scavenging of Hydrogen Peroxide and Methylglyoxal but Not Superoxide or Nitric Oxide in Tobacco Cultured Cells

Banu

Hoque

Watanabe-Sugimoto

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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“…42 Tobacco plants overexpressing glyoxalase are resistant to increased level of MG and maintain a highly reduced glutathione level under salinity stress. 42 APX, MADR, and GPX are key enzymes detoxifying H 2 O 2 to H 2 O by ascorbate and GSH. 9 The identified TRX (spot 2328) was the h type TRX and together with glutathione is a key thiol buffer that functions mainly in reducing levels of disulfides.…”

Section: Xu Et Almentioning

confidence: 99%

Integrated Proteomic and Cytological Study of Rice Endosperms at the Storage Phase

Yan

et al. 2010

J. Proteome Res.

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The endosperm at the storage phase undergoes a series of coordinated cellular and metabolic events, including starchy endosperm cell death, starch synthesis, and starch granule packaging, which leads to efficient accumulation of starch. However, the mechanism underlying the interconnections remains unknown. We used integrated proteomic and cytological approaches to probe the interconnections in rice (Oryza sativa) endosperm at the storage phase from 12 to 18 days after flowering (DAF). Starch granule packaging was completed first in the inner part of endosperm at 15 DAF and spread to almost the entire endosperm at 18 DAF. Programmed starchy endosperm cell death occurred after the starch granule packaging. Endogenous H 2 O 2 was detectable in the inner part of endosperm at 12 DAF and the region beyond the inner part at 15 DAF, with an H 2 O 2 burst at 15 DAF. Proteomics analysis with 2-D fluorescent difference gel electrophoresis and matrix-assisted laser-desorption ionization time-of-flight/ time-of-flight mass spectrometry revealed 317 proteins, including almost all known antioxidants, differentially expressed throughout the 3 stages of the developmental phase. More than two-thirds of the 317 proteins were potential thioredoxin targets, with a preferential skew toward central carbon metabolism, alcoholic fermentation, starch metabolism, amino acid metabolism, and protein synthesis or folding. These proteins implicated in starch synthesis and gluconeogenesis were upregulated, whereas those involved in anabolism of biomacromolecules such as proteins, lipids, and cell wall components were downregulated, with upregulated expression of proteins involved in catabolism of these biomacromolecules, which suggests remobilization of nutrients for starch synthesis. These data suggested important roles of the H 2 O 2 -antioxidant interface in coordinating starch accumulation, programmed cell death of starchy endosperm, and remobilization of nutrients during the cell death.

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Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione

Cited by 404 publications

References 27 publications

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

GOverexpression of the Glyoxalase II Gene Leads to Enhanced Salinity Tolerance in Brassica Juncea

Proline and Glycinebetaine Ameliorated NaCl Stress via Scavenging of Hydrogen Peroxide and Methylglyoxal but Not Superoxide or Nitric Oxide in Tobacco Cultured Cells

Integrated Proteomic and Cytological Study of Rice Endosperms at the Storage Phase

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