Methylenetetrahydrofolate reductase polymorphism C677T is not associated to the risk of cervical dysplasia

Association between MTHFR C677T, MTHFR A1298C and MS A2756G polymorphisms and risk of cervical intraepithelial neoplasia II/III and cervical cancer: A meta-analysis

“…Moreover, 1 study (8) was replaced with its updated version since the subjects in these 2 studies were from the same population. The final pool of eligible studies consisted of 13 studies with 1,936 cases and 2,858 controls (3,6,12,13,15,17,(21)(22)(23)(24)(25)(26)(27) for MTHFR C677T polymorphism (Table I), 5 studies with 585 cases and 1,000 controls for MTHFR A1298C polymorphism (Table I), and 3 studies with 389 cases and 440 controls for MS A2756G polymorphism (Table I). The genotype distribution in the controls of all these studies was consistent with HWE.…”

“…One study (8) was replaced with its updated version due to overlapping inclusion of study subjects in the most recent study. The eligible studies comprised 7 studies (3,6,17,21,24,26,27) with 1,936 cases and 2,858 controls for MTHFR C677T polymorphism (Table I) and the genotype distribution in the controls of all these studies was consistent with HWE. There were not enough studies available for the meta-analysis of the contribution of MTHFR A1298C or MS A2756G polymorphisms to susceptibility to CIN II/III.…”

“…DNA was prepared from blood samples or tissue for genotyping in all the studies. SNaPshot genotyping assay was adopted in 2 studies (3,21) and a TaqMan single nucleotide polymorphism (SNP) genotyping assay was used in 2 studies (6,17) with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) employed in all of the remaining studies (13,15,(22)(23)(24)(25)(26)(27) to validate genotype distribution (Table I).…”

“…Distribution of MS A2756G genotypes and their allelic frequency associated with the risk of cervical cancer. The gene for MTHFR is located on chromosome 1p36 with 11 exons (44) and SNPs within the coding region are associated with DNA hypomethylation, which constitutes a hallmark of human cancer cells (1,24,25). Two common mutations in the MTHFR gene are C677T and A1298C.…”

Methylenetetrahydrofolate Reductase C677T Polymorphism and Cervical Cancer Risk: a Meta-Analysis

“…After discarding those which clearly did not meet the criteria, 19 studies were further assessed for eligibility (Piyathilake et al, 2000;Goodman et al, 2001;Gerhard et al, 2003;Lambropoulos et al, 2003;Sull et al, 2004;Kang et al, 2005;Zoodsma et al, 2005;Delgado-Enciso et al, 2006;Rao et al, 2006;Piyathilake et al, 2007;Nandan et al, 2008;Shekari et al, 2008;Agodi et al, 2010;Kohaar et al, 2010;Tong et al, 2010;Mostowska et al, 2011;Prasad and Wilkhoo, 2011;Tong et al, 2011;von Keyserling et al, 2011). After reviewing each original paper and extracting data, nine studies were excluded including five studies focusing on cervical intraepithelial neoplasia (Piyathilake et al, 2000;Goodman et al, 2001;Lambropoulos et al, 2003;Piyathilake et al, 2007;Agodi et al, 2010), three studies for not specifying genotypic frequencies of CC, CT, and TT (Gerhard et al, 2003;Nandan et al, 2008;Rao et al, 2006) and one study for overlapping data (Tong et al, 2010). Finally, 10 casecontrol studies with a total of 2113 cervical cancer cases and 2804 controls were included into this meta-analysis (Sull et al, 2004;Kang et al, 2005;Zoodsma et al, 2005;Delgado-Enciso et al, 2006;Shekari et al, 2008;Kohaar et al, 2010;Mostowska et al, 2011;...…”