Methylene Chloride Fraction of Spatholobi Caulis Induces Apoptosis via Caspase Dependent Pathway in U937 Cells

Ha, Eun-Suk; Lee, Eun-Ok; Yoon, Taek-Joon; Kim, Jinhyung; Park, Jong-Oh; Lim, Nak-Cheol; Jung, Sung Il; Yoon, Byung-Soo; Kim, Sung Hoon

doi:10.1248/bpb.27.1348

Cited by 21 publications

(10 citation statements)

References 25 publications

(28 reference statements)

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“…It also inhibited HT1080 cancer cell invasion through a Matrigel-coated filter with an IC50 of 25 mg/ml [12]. SSCE was also found to induce apoptosis via the caspase-dependent pathway in U937 cells [13]. In line with the previous reports, we found similar antiproliferation effect of Spatholobi Caulis extract (SSCE) in MCF-7 cells with an IC50 value of 109 μ g/ml.…”

Section: Discussionsupporting

confidence: 88%

Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Sun

Zhang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Although Chinese herbal compounds have long been alternatively applied for cancer treatment in China, their treatment effects have not been sufficiently investigated. The Chinese herb Spatholobus suberectus is commonly prescribed to cancer patients. HPLC analysis has shown that the main components of Spatholobus suberectus are flavonoids that can be classified as phytoestrogens, having a structure similar to estrogen. This study was designed to investigate the effects of Spatholobus suberectus column extract (SSCE) on the estrogen receptor-positive (ER+) breast cancer cell line MCF-7 and its possible molecular mechanism. In our study, MTT assay was performed to evaluate cell viability. The results show that SSCE (80, 160, and 320 μg/ml) significantly decreased the viability of MCF-7 cells. SSCE also triggered apoptosis, arrested the cell cycle at the G0/G1 phase, and inhibited cell migration. A dual-luciferase reporter system showed that SSCE suppressed intranuclear p-ER activity; Western blot analysis confirmed the repressed expression of phosphorylated-ER alpha (p-ERα), ERK1/2, p-ERK1/2, AKT, p-AKT, p-mTOR, PI3K, and p-PI3K, indicating that SSCE suppressed the MAPK PI3K/AKT signaling pathway. Collectively, our results suggest that SSCE causes apoptosis, an arrest in the G0/G1 phase, and a decrease in migration in ER+ MCF-7 cells via hypoactivity of the ER and suppression of the MAPK PI3K/AKT pathway.

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Section: Discussionsupporting

confidence: 88%

Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Sun

Zhang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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“…Salvia miltiorrhiza , another traditional herbal medicine, classified similarly with DD in terms of blood circulatory effects, induces apoptosis and necrosis in a ROS-independent and caspase-independent manner in acute lymphoblastic leukemia cells ( 80 ). Additionally, Spatholobi caulis , an effective Chinese medicine for relieving blood stasis, was proved to exert caspase-dependent apoptotic activity on U937, a human monocyte leukemia cell line ( 81 ). In previous studies, compared with the efficacy of natural products targeting a single mechanism for leukemia caused by complex mutations, DD significantly activated mitochondria-mediated caspase activation, ER stress-related regulation of ROS, c-Jun, and miR-216b, indicating the multiple anticancer mechanisms of DD ( Figure 7 ).…”

Section: Discussionmentioning

confidence: 99%

Daemonorops draco Blume Induces Apoptosis Against Acute Myeloid Leukemia Cells via Regulation of the miR-216b/c-Jun

et al. 2022

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Daemonorops draco Blume (DD), also called dragon’s blood, has been used as a traditional Korean medicine, especially for relieving pain caused by wound infection. Recently, it has been described that DD has antibacterial and analgesic effects. In this study, the underlying anticancer effect of DD associated with apoptosis was investigated in acute myeloid leukemia cell lines U937 and THP-1. DD exhibited cytotoxic effects and induced apoptosis in U937 and THP-1 cells. Moreover, DD treatment significantly reduced mitochondrial membrane potential (ΔΨ). The protein expression of cleaved poly(ADP-ribose) polymerase, cleaved caspase-3, p-H2A.X, CCAAT/enhancer-binding protein (CHOP), and activating transcription factor 4 was upregulated by DD treatment. Consistently, DD-treated cells had increased reactive oxygen species (ROS) level in a concentration-dependent manner via miR-216b activation in association with c-Jun inhibition. N-acetyl-L-cysteine pretreatment reversed the cytotoxic effect of DD treatment as well as prevented ROS accumulation. Collectively, the results of this study suggest that the anticancer effect of DD in AML was mediated by CHOP-dependent apoptosis along with ROS accumulation and included upregulation of miR-216b followed by a decrease in c-Jun.

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“…Spatholobi Caulis (SC) is a traditional Chinese herb produced from the vine stem of Spatholobus suberectus Dunn and has been shown to have anti-inflammatory, anti-osteoarthritic, anti-anemic, antiviral, and neuroprotective effects [11][12][13][14][15]. Furthermore, the cytotoxicity of SC was reported on various cancerous cell lines, such as monocyte leukemia, cervical cancer, osteosarcoma cancer, and colorectal cancer [16][17][18][19][20]. Phenolic compounds, including flavanones, flavan-3-ol, chalcone, isoflavonoids, and phenolic acids, are considered the primary bioactive constituent compounds of SC [21,22] expressing anticancer activity against several cancer cell lines [23].…”

Section: Introductionmentioning

confidence: 99%

Analysis and Anticancer Effects of Active Compounds from Spatholobi Caulis in Human Breast Cancer Cells

Phung

Lee

et al. 2020

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Breast cancer is the most common malignancy in both developing and developed countries. In this study, we simultaneously analyzed nine constituent compounds from Spatholobi Caulis (gallic acid, (−)-gallocatechin, 3,4-dihydroxybenzoic acid, procyanidin B1, 3,4-dihydroxybenzaldehyde, catechin, procyanidin B2, epicatechin, and (−)-epicatechin gallate) and examined their anticancer effects on MCF-7 and MDA-MB-231 human breast cancer cells. The experimental results indicated that the gallic acid showed the strongest cytotoxic effect on MCF-7 cells among tested compounds whilst most of samples did not express inhibitory effect on viability of MDA-MB-231 cells, except for 70% ethanol extract of S. Caulis. Thus, gallic acid was chosen to extend anticancer mechanism study on MCF-7 cells. Our data showed that the gallic acid induced apoptotic MCF-7 cell death through both extrinsic and intrinsic pathways, which increased the expression of cleaved caspase-7, -8, and -9, Bax and p53, but reduced the expression of Bcl-2 and poly (ADP-ribose) polymerase (PARP). In addition, the network pharmacological analysis pointed out that the p53, mitogen-activated protein kinase (MAPK), estrogen, and Wnt signaling pathways have a great correlation with the targets of gallic acid. This study suggested that gallic acid is a bioactive component of S. Caulis with potential to be used in chemotherapy for breast cancer.

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Methylene Chloride Fraction of Spatholobi Caulis Induces Apoptosis via Caspase Dependent Pathway in U937 Cells

Cited by 21 publications

References 25 publications

Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Daemonorops draco Blume Induces Apoptosis Against Acute Myeloid Leukemia Cells via Regulation of the miR-216b/c-Jun

Analysis and Anticancer Effects of Active Compounds from Spatholobi Caulis in Human Breast Cancer Cells

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