Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, <i>Spirulina platensis</i> NIES‐39

Tanioka, Yuri; Miyamoto, Emi; Yabuta, Yukinori; Ohnishi, Kouhei; Fujita, Tomoyuki; Yamaji, Ryoichi; Misono, Haruo; Shigeoka, Shigeru; Nakano, Yoshihisa; Inui, Hiroshi; Watanabe, Fumio

doi:10.1016/j.febslet.2010.06.013

Cited by 38 publications

(29 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SCM1, HCE1, HCA1, and PS0) also produced cobalamin (Table S1), confirming earlier suggestions based on the presence of cobalamin biosynthesis genes in Thaumarchaeota genomes (10). Like other Cyanobacteria (11,13,14), four axenic strains of marine Cyanobacteria (Prochlorococcus MED4 and MIT9313 and Synechococcus WH8102 and WH7803) produced pseudocobalamin (Table S1). In all of the cobalamin or pseudocobalamin producers, we detected compounds with β ligands Me-, Ado-, and OH-but not CN- (Table S1).…”

supporting

confidence: 86%

“…The 3D structure of MetH contains three β pleated sheets and two α helices that form a pocket for the DMB ligand of cobalamin in Escherichia coli (31,32). Cyanobacterial MetH is predicted to form the same pocket (13). However, conserved amino acids within this pocket in the cyanobacterial MetH differ from sequences of organisms known to use cobalamin (Figs.…”

Section: All Of the 49mentioning

confidence: 99%

“…1). Previous studies have shown that instead of producing cobalamin, Cyanobacteria produce pseudocobalamin (11,13,14), an analog of cobalamin in which adenine substitutes for DMB as the α ligand (12) (Fig. 1).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Two distinct pools of B₁₂analogs reveal community interdependencies in the ocean

Heal

Qin

Ribalet

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

161

224

View full text Add to dashboard Cite

Organisms within all domains of life require the cofactor cobalamin (vitamin B 12 ), which is produced only by a subset of bacteria and archaea. On the basis of genomic analyses, cobalamin biosynthesis in marine systems has been inferred in three main groups: select heterotrophic Proteobacteria, chemoautotrophic Thaumarchaeota, and photoautotrophic Cyanobacteria. Culture work demonstrates that many Cyanobacteria do not synthesize cobalamin but rather produce pseudocobalamin, challenging the connection between the occurrence of cobalamin biosynthesis genes and production of the compound in marine ecosystems. Here we show that cobalamin and pseudocobalamin coexist in the surface ocean, have distinct microbial sources, and support different enzymatic demands. Even in the presence of cobalamin, Cyanobacteria synthesize pseudocobalaminlikely reflecting their retention of an oxygen-independent pathway to produce pseudocobalamin, which is used as a cofactor in their specialized methionine synthase (MetH). This contrasts a model diatom, Thalassiosira pseudonana, which transported pseudocobalamin into the cell but was unable to use pseudocobalamin in its homolog of MetH. Our genomic and culture analyses showed that marine Thaumarchaeota and select heterotrophic bacteria produce cobalamin. This indicates that cobalamin in the surface ocean is a result of de novo synthesis by heterotrophic bacteria or via modification of closely related compounds like cyanobacterially produced pseudocobalamin. Deeper in the water column, our study implicates Thaumarchaeota as major producers of cobalamin based on genomic potential, cobalamin cell quotas, and abundance. Together, these findings establish the distinctive roles played by abundant prokaryotes in cobalamin-based microbial interdependencies that sustain community structure and function in the ocean. vitamin B 12 ) is synthesized by a select subset of bacteria and archaea, yet organisms across all domains of life require it (1-3). In the surface ocean, cobalamin auxotrophs (including most eukaryotic algae) (3) obtain the vitamin through direct interactions with cobalamin producers (3) or breakdown of cobalamin-containing cells (4,5). Interdependencies between marine cobalamin producers and consumers are critical in surface waters where primary productivity can be limited by the availability of cobalamin and the compound is short-lived (1, 6, 7). The exchange of cobalamin in return for organic compounds is hypothesized to underpin mutualistic interactions between heterotrophic bacteria and autotrophic algae (3,6,8,9). The apparent pervasiveness of cobalamin biosynthesis genes in chemoautotrophic Thaumarchaeota and photoautotrophic Cyanobacteria genomes (1, 10, 11) raises the question of whether cobalamin production by these autotrophs may underlie additional, unexplored microbial interactions.Cobalamin is a complex molecule with a central cobalt-containing corrin ring, an α ligand of 5,6-dimethylbenzimidizole (DMB), and a β ligand of either OH-, CN-, Me-, or Ado-(12) (Fig. 1). Pr...

show abstract

supporting

confidence: 86%

Section: All Of the 49mentioning

confidence: 99%

See 1 more Smart Citation

Two distinct pools of B₁₂analogs reveal community interdependencies in the ocean

Heal

Qin

Ribalet

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

161

224

View full text Add to dashboard Cite

show abstract

“…Our results indicated that because naturally grown N. flagelliforme contain substantial amounts of pseudo B 12 , which is inactive in humans [7] and because the fake items have very low B 12 contents, commercially available hair vegetable is not suitable for use of B 12 source, regardless of the presence of the fake items. Cyanobacteria have the ability to synthesize pseudo B 12 [13], which functions as a coenzyme of methionine synthase to catalyze the synthesis of methionine from homocysteine and N 5 -methyltetrahydrofolate [14]. In the present study, the cultured Nostoc sample I predominantly contained B 12 but not pseudo B 12 ( Table 2), suggesting that N. flagelliforme may synthesize both B 12 and pseudo B 12 de novo.…”

Section: Evaluation Of True and Fake N Flagelliformementioning

confidence: 57%

Characterization of Corrinoid Compounds in the Edible Cyanobacterium <i>Nostoc flagelliforme</i> the Hair Vegetable

Teng¹,

Bito²,

Takenaka³

et al. 2014

FNS

Self Cite

View full text Add to dashboard Cite

Vitamin B 12 contents in the edible cyanobacterium Nostoc flagelliforme, also known as hair vegetable, were assayed using a microbiological method. We detected high vitamin B 12 contents in samples of naturally grown cells (109.2 ± 18.5 μg/100g dry weight) and cultured cells (120.2 ± 53.6 μg/100g dry weight). However, commercially available hair vegetable samples, which comprised fake substitutes and Nostoc, had variable contents (4.8 -101.6 μg/100g dry weight) because concomitant fake items contain very low vitamin B 12 contents. To evaluate whether natural and cultured N. flagelliforme samples contained vitamin B 12 or pseudovitamin B 12 , corrinoid compounds were purified and identified as pseudovitamin B 12 (approximately 72%) and vitamin B 12 (approximately 28%) using silica gel 60 TLC bioautography and LC/MS. The results suggested that N. flagelliforme contains substantial amounts of pseudovitamin B 12 , which is inactive in humans.

show abstract

“…(127.2-244.3 g) (4), Suizenji-nori (Aphanothece sacrum, 143.8 g) (8), Ishikurage (Nostoc commune, 98.8 g) (9). Cyanobacteria have the ability to synthesize corrinoids (10), which function as a coenzyme of methionine synthase (11).…”

Section: Resultsmentioning

confidence: 99%

Characterization of Corrinoid Compounds from Edible Cyanobacterium Nostochopsis sp.

Hashimoto

Yabuta

Takenaka

et al. 2012

J Nutr Sci Vitaminol

Self Cite

View full text Add to dashboard Cite

Summary Vitamin B 12 content of an edible cyanobacterium, Nostochopsis sp. was determined to be 140.6 Ϯ 16.2 g/100 g dry weight by a microbiological method. To evaluate whether the Nostochopsis cells contain vitamin B 12 or inactive corrinoid compounds, corrinoid compounds were purified from the cells and then identified as pseudovitamin B 12 (97.4 Ϯ 11.8 g/100 g dry weight) and vitamin B 12 (43.2 Ϯ 6.0 g/100 g dry weight) on the basis of silica gel 60 TLC bioautograms and LC/ESI-MS/MS chromatograms. Vitamin B 12 content was significantly increased in the Nostochopsis cells (254.8 Ϯ 17.6 g/100 g dry weight) grown in the vitamin B 12 -supplemented medium.

show abstract

Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, Spirulina platensis NIES‐39

Cited by 38 publications

References 19 publications

Two distinct pools of B₁₂analogs reveal community interdependencies in the ocean

Two distinct pools of B₁₂analogs reveal community interdependencies in the ocean

Characterization of Corrinoid Compounds in the Edible Cyanobacterium <i>Nostoc flagelliforme</i> the Hair Vegetable

Characterization of Corrinoid Compounds from Edible Cyanobacterium Nostochopsis sp.

Contact Info

Product

Resources

About

Methyladeninylcobamide functions as the cofactor of methionine synthase in a Cyanobacterium, Spirulina platensis NIES‐39

Cited by 38 publications

References 19 publications

Two distinct pools of B12analogs reveal community interdependencies in the ocean

Two distinct pools of B12analogs reveal community interdependencies in the ocean

Characterization of Corrinoid Compounds in the Edible Cyanobacterium &lt;i&gt;Nostoc flagelliforme&lt;/i&gt; the Hair Vegetable

Characterization of Corrinoid Compounds from Edible Cyanobacterium Nostochopsis sp.

Contact Info

Product

Resources

About

Two distinct pools of B₁₂analogs reveal community interdependencies in the ocean

Two distinct pools of B₁₂analogs reveal community interdependencies in the ocean

Characterization of Corrinoid Compounds in the Edible Cyanobacterium <i>Nostoc flagelliforme</i> the Hair Vegetable