Methyl tert.-butyl ether: A new chromatographic eluent

Little, C.J.; Dale, A.D.; Whatley, J. A.; Wickings, J.A.

doi:10.1016/0021-9673(75)85064-3

Cited by 48 publications

(9 citation statements)

References 1 publication

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“…Buffer saturated MTBE with HCN was the solvent, with this single phase system the chemical background reaction is essentially suppressed in the solvent. Moreover, MTBE is much safer than the commonly used diisopropyl ether since it forms essentially no explosive peroxides [21,22]. As the reaction system, the immobilized PaHNL on Celite R-633 was utilized in a teabag to ensure straightforward recycling.…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite

et al. 2018

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Abstract:The hydroxynitrile lyase from Prunus amygdalus was immobilized on Celite R-633. The immobilized enzyme could successfully be utilized in buffer saturated MTBE and excellent conversions of benzaldehyde to R-mandelonitrile were observed. No leaching occurred. To achieve high enantioselectivities, the suppression of the undesired background reaction was essential. This could be achieved by high enzyme loadings and the tight packing of the immobilized enzymes. When the immobilized enzyme is loosely packed, both the enzyme catalysis and the background reaction accelerates and only a modest enantioselectivity is observed. The enzyme was recycled for up to ten times, with some loss of activity and also enantioselectivity after 5 cycles, independent of packing.

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Section: Resultsmentioning

confidence: 99%

Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite

et al. 2018

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“…Three extraction solvents were used in order to compare the extraction efficiency of triflusal and HTB from plasma. Solvent A was acetonitrile-chloroform (40:60, v/v) [5], solvent B was diethylether [2] and solvent C was tert-butyl methyl ether (TBME) [6]. For a human plasma sample (0.5 ml), an aliquot of 100 l of the IS (1 g/ml of furosemide) and 200 l of 0.1 M hydrochloric acid were immediately added and mixed with vortex-mixer for 30 s. For a rat plasma sample (0.1 ml), an aliquot of 100 l of the IS (0.5 g/ml of furosemide) and 100 l of 0.1 M hydrochloric acid were used and mixed.…”

Section: Extraction Proceduresmentioning

confidence: 99%

“…HTB corresponds to 2-hydroxy benzoic acid, the major circulating metabolite of aspirin. Thus, the analysis of triflusal and HTB is mainly based on the analysis of aspirin and salicylic acid [6,[10][11][12]. There are two problems associated with the HPLC analysis of triflusal and HTB in biological fluids like aspirin and salicylic acid.…”

Section: Chromatographic Analysismentioning

confidence: 99%

Simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography

Cho

Jeong

Lee

2003

Journal of Chromatography B

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“…MTBE is an alkyl ether similar to diethyl ether, an anesthetic agent. Unlike the lower boiling point of diethyl ether (35 °C), MTBE has a relatively higher boiling point (55 °C); thus, it is liquid at body temperature [2, 3]. Acceptable dissolubility of MTBE was validated by a variety of studies, including a large survey of 803 patients across 21 European hospitals that demonstrated a dissolution rate of 96.6% (724/804) with a low rate of toxic side effects and complications [4].…”

Section: Introductionmentioning

confidence: 99%

Efficacy and safety of a novel topical agent for gallstone dissolution: 2-methoxy-6-methylpyridine

et al. 2019

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Background Although methyl-tertiary butyl ether (MTBE) is the only clinical topical agent for gallstone dissolution, its use is limited by its side effects mostly arising from a relatively low boiling point (55 °C). In this study, we developed the gallstone-dissolving compound containing an aromatic moiety, named 2-methoxy-6-methylpyridine (MMP) with higher boiling point (156 °C), and compared its effectiveness and toxicities with MTBE. Methods The dissolubility of MTBE and MMP in vitro was determined by placing human gallstones in glass containers with either solvent and, then, measuring their dry weights. Their dissolubility in vivo was determined by comparing the weights of solvent-treated gallstones and control (dimethyl sulfoxide)-treated gallstones, after directly injecting each solvent into the gallbladder in hamster models with cholesterol and pigmented gallstones. Results In the in vitro dissolution test, MMP demonstrated statistically higher dissolubility than did MTBE for cholesterol and pigmented gallstones (88.2% vs. 65.7%, 50.8% vs. 29.0%, respectively; P < 0.05). In the in vivo experiments, MMP exhibited 59.0% and 54.3% dissolubility for cholesterol and pigmented gallstones, respectively, which were significantly higher than those of MTBE (50.0% and 32.0%, respectively; P < 0.05). The immunohistochemical stains of gallbladder specimens obtained from the MMP-treated hamsters demonstrated that MMP did not significantly increase the expression of cleaved caspase 9 or significantly decrease the expression of proliferation cell nuclear antigen. Conclusions This study demonstrated that MMP has better potential than does MTBE in dissolving gallstones, especially pigmented gallstones, while resulting in lesser toxicities. Electronic supplementary material The online version of this article (10.1186/s12967-019-1943-y) contains supplementary material, which is available to authorized users.

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Methyl tert.-butyl ether: A new chromatographic eluent

Cited by 48 publications

References 1 publication

Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite

Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite

Simultaneous determination of triflusal and its major active metabolite, 2-hydroxy-4-trifluoromethyl benzoic acid, in rat and human plasma by high-performance liquid chromatography

Efficacy and safety of a novel topical agent for gallstone dissolution: 2-methoxy-6-methylpyridine

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