Methyl jasmonate represses growth and affects cell cycle progression in cultured Taxus cells

Abstract

Methyl jasmonate (MeJA) elicitation is an effective strategy to induce and enhance synthesis of the anticancer agent paclitaxel (Taxol®) in Taxus cell suspension cultures; however, concurrent decreases in growth are often observed, which is problematic for large scale bioprocessing. Here, increased accumulation of paclitaxel in Taxus cuspidata suspension cultures with MeJA elicitation was accompanied by a concomitant decrease in cell growth, evident within the first three days post-elicitation. Both MeJA-elicited and mock-elicited cultures exhibited similar viability with no apoptosis up to day 16 and day 24 of the cell culture period, respectively, suggesting that growth repression is not attributable to cell death. Flow cytometric analyses demonstrated that MeJA perturbed cell cycle progression of asynchronously dividing Taxus cells. MeJA slowed down cell cycle progression, impaired the G1/S transition as observed by an increase in G0/G1 phase cells, and decreased the number of actively dividing cells. Through a combination of deep sequencing and gene expression analyses, the expression status of Taxus cell cycle-associated genes correlated with observations at the culture level. Results from this study provide valuable insight into the mechanisms governing MeJA perception and subsequent events leading to repression of Taxus cell growth.

A cellulase-supported two-phase in situ system for enhanced biosynthesis of paclitaxel in Taxus × media hairy roots

“…These observations correspond to the results of previous investigations (Sykłowska- Baranek et al 2015b), where root growth in PFD supported cultures after elicitation with MJ was higher than in that elicited without perfluorinated phases, degassed or aerated. The results of current study are also consistent with data published by Patil et al (2014) which indicates that cell growth in suspension cultures of T. cuspidata upon elicitation with MJ could be attributed to slowed down cell cycle progression, an increase in G0/G1 phase cells and a reduced number of actively dividing cells rather than necrosis.…”

Transcriptome profiling of the salt-stress response in Triticum aestivum cv. Kharchia Local

“…Large number of studies on the high throughput sequencing of plant transcriptome in many model and non model species, such us maize, grapevine, Taxus , eucalyptus, olive and cucumber etc. have been undertaken1920212223. Comparative global transcriptome analysis is a powerful approach for discovering the molecular basis underlying specific biological events242526.…”

Jasmonic acid stimulates the oxidative responses and triterpene production in Jatropha curcas cell suspension cultures through mevalonate as biosynthetic precursor

“…It has been reported that the time of elicitation is one of the key factors that affect cell growth and production of secondary metabolites in plant cell suspension cultures. Thus, in Taxus cell cultures, the repression of the growth through inhibition of cell cycle progression by MeJA elicitation occurs accompanied by the increased accumulation of paclitaxel (Patil et al 2014). In Panax notoginseng cell cultures, elicitation with jasmonate derivates during the early exponential phase of growth up-regulate squalene synthase and squalene epoxidase, two common enzymes of triterpene and sterol biosynthesis, but down-regulate the sterol related cycloartenol synthase transcription, which implies that oxidosqualene can be directed from sterol to ginsenoside biosynthesis (Hu and Zhong 2008).…”