Methyl jasmonate and yeast extract stimulate mitragynine production in Mitragyna speciosa (Roxb.) Korth. shoot culture

Wungsintaweekul, Juraithip; Choo-malee, Jutarat; Charoonratana, Tossaton; Keawpradub, Niwat

doi:10.1007/s10529-012-0968-6

Cited by 19 publications

(12 citation statements)

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“…The induction mechanism of elicitors (both biotic and abiotic) is generally regarded as activating the expression of defense-related genes. The treatment with methyl jasmonate has been a useful strategy to enhance bioactive compounds production in in vitro shoot cultures of Catharanthus roseus (vindoline), D. burmanii (plumbagin increased 3-fold), Mitragyna speciosa (mitragynine increased 3-fold), Bacopa monnieri (bacoside A increased 1.8-fold), Withania somnifera (withanolide A increased 14-fold, withanolide B 11-fold, withaferin A 13-fold, withanone 12-fold) (Vazquez-Flota et al, 2009;Putalun et al, 2010;Wungsintaweekul et al, 2012;Sharma et al, 2013;Sivanandhan et al, 2013). The elicitation with yeast extract has been employed for secondary metabolites enhancement in in vitro shoot cultures of Centella asiatica (asiaticoside increased 1.4-fold), D. burmanii (plumbagin increased 3.5-fold), M. speciosa (mitragynine) (Kim et al, 2004;Putalun et al, 2010;Wungsintaweekul et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures

Kikowska

Kędziora

Krawczyk³

et al. 2015

Acta Biochim Pol

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Eryngium planum L. has been reported as a medicinal plant used in traditional medicine in Europe. The tissue cultures may be an alternative source of the biomass rich in desired bioactive compounds. The purpose of this study was to investigate the influence of the biotechnological techniques on the selected phenolic acids accumulation in the agitated shoot cultures of E. planum. Qualitative and quantitative analyses of those compounds in 50% aqueous - methanolic extracts from the biomass were conducted by applying the HPLC method. Methyl jasmonate (MeJA), yeast extract (YE) and sucrose (Suc) stimulated accumulation of the phenolic acids: rosmarinic (RA), chlorogenic (CGA) and caffeic (CA) in in vitro shoot cultures. Cultivation of shoots in liquid MS media supplemented with 1.0 mg L(-1) 6-benzyladenine and 0.1 mg L(-1) indole-3-acetic acid in the presence of 100 µM MeJA for 48h was an optimum condition of elicitation and resulted in approximately 4.5-fold increased content of RA + CGA + CA in plant material compared to the control (19.795 mg g(-1) DW, 4.36 mg g(-1) DW, respectively). The results provide the first evidence that the selected phenolic acids can be synthesized in elicited shoot cultures of flat sea holly in higher amount than in untreated shoots.

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Section: Resultsmentioning

confidence: 99%

Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures

Kikowska

Kędziora

Krawczyk³

et al. 2015

Acta Biochim Pol

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“…This conclusion was confi rmed by the results of feeding tryptamine or loganin. Although the metabolite profi le of M. speciosa shoot culture is different from that of the intact plant, the cultures have the capacity to produce mitragynine (Wungsintaweekul et al, 2012). At 0.2 mM and 0.4 mM of tryptamine or loganin, mitragynine production in the shoot culture reacted more sensitively to tryptamine than to loganin, and even more to the combination of both (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The shoot cultures of M. speciosa were initiated from axillary buds of the two-month-old M. speciosa plantlets as described previously (Wungsintaweekul et al, 2012). The axillary buds were cut off and placed on WPM medium supplemented with 2 mg/L TDZ and 1 mg/L BA.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Recently, the regulation of M. speciosa strictosidine synthase has been investigated and found to respond to high concentrations of salicylic acid (Jumali et al, 2011). Application of methyl jasmonate and yeast extract to M. speciosa shoot cultures increased the transcript levels of tryptophan decarboxylase and strictosidine synthase (Wungsintaweekul et al, 2012). Since mitragynine is a compound of medicinal importance, the regulation of the mitragynine biosynthesis is an attractive area of research for advances in biotechnological applications.…”

Section: Introductionmentioning

confidence: 99%

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Limitation of Mitragynine Biosynthesis in Mitragyna speciosa (Roxb.) Korth. through Tryptamine Availability

Charoonratana

Wungsintaweekul

Pathompak

et al. 2013

Zeitschrift Für Naturforschung C

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Metabolite profi les of Mitragyna speciosa were determined by means of 1 H NMR-based and HPLC-based analyses. The results indicated that high contents of secologanin, caffeic acid, gallic acid, epigallocatechin, and mitragynine were accumulated in leaves. In M. speciosa, feedings of tryptamine, tryptophan, phenylalanine or tyrosine signifi cantly increased the mitragynine contents. Feedings of tryptamine and loganin also enhanced the mitragynine accumulation, but feeding of loganin only did not affect the mitragynine level. The mRNA levels of anthranilate synthase alpha subunit (ASA), tryptophan decarboxylase (TDC), and strictosidine synthase (STR) were measured by quantitative real-time polymerase chain reaction (RT-qPCR) in control plants and those exposed to methyl jasmonate (MJ; 10 μM). All genes responded to MJ after a 24-h treatment. The mitragynine contents were also enhanced and corresponded to the transcript levels. From the present results we conclude that a high content of secologanin together with a undetectable level of tryptamine in M. speciosa feature the limitation of mitragynine biosynthesis. Additionally, expression of all the genes limits production of an essential precursor for mitragynine production.

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“…As xanthones were reported mostly from aerial parts of H. fastigiata, we anticipated that establishment of shoot cultures could be a suitable platform for production of xanthones [4]. This raison d'être is based on the expression of pathway enzymes between disorganized and organized cultures, as elucidated with Mitragyna speciosa shoot cultures [20].…”

Section: Introductionmentioning

confidence: 99%

Shoot cultures of Hoppea fastigiata (Griseb.) C.B. Clarke as potential source of neuroprotective xanthones

et al. 2015

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Hoppea fastigiata, an annual medicinal herb belonging to the Gentianaceae, is mostly found in South Asian countries, and is used by local tribes for various brain-related ailments. The genus possesses a unique class of compounds, xanthones, which are known for their potential against Alzheimer's and Parkinson's diseases. Limited availability and the potential pharmacological significance of the plants has led to the establishment of in vitro cultures of H. fastigiata and study of its neuroprotective principles. In vitro plantlets were established from the apical meristem of the plant in Murashige and Skoog medium with a combination of the phytohormones 6-benzylaminopurine (1 mg/L) and kinetin (0.1 mg/L), which was found to be efficacious with a growth index of 0.9 ± 0.01 after 30 days. Four different solvent extracts of in vitro cultures were evaluated for acetylcholinesterase (AChE) and monoamine oxidase A and B (MAO-A and MAO-B) inhibitory activities, amongst which the ethanolic extract showed the lowest IC50 value in all the assays. Three major compounds were isolated from the ethanolic extract and structurally confirmed as 1,5,7-trihydroxy-3-methoxyxanthone (1), 1,5-dihydroxy-3,7-dimethoxyxanthone (2) and 1,3,5-trihydroxy-8-methoxyxanthone (3). Compound 3 showed the strongest AChE inhibitory activity with mixed-type inhibition. Compounds 1 and 2 also showed promising AChE inhibitory properties with mixed and non-competitive types of inhibition, respectively. Compounds 1 and 2 showed inhibition of MAO-A (mixed and competitive, respectively) and compounds 2 and 3 showed inhibition of MAO-B (competitive and mixed, respectively). Extracts and isolated compounds showed good antioxidant capacities. The ethanolic extract and compound 2 showed the strongest antioxidant activities among the other solvent extracts and compounds, respectively.

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Methyl jasmonate and yeast extract stimulate mitragynine production in Mitragyna speciosa (Roxb.) Korth. shoot culture

Cited by 19 publications

References 12 publications

Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures

Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures

Limitation of Mitragynine Biosynthesis in Mitragyna speciosa (Roxb.) Korth. through Tryptamine Availability

Shoot cultures of Hoppea fastigiata (Griseb.) C.B. Clarke as potential source of neuroprotective xanthones

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