Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures

Kikowska, Małgorzata; Kędziora, Izabela; Krawczyk, A.; Thiem, Barbara

doi:10.18388/abp.2014_880

Cited by 27 publications

(14 citation statements)

References 16 publications

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“…In shoots cultured on the solid MS medium, the content of rosmarinic acid was 3.5-times higher and of caffeic acid it was 24.33-times higher than in basal leaves of the intact plant (Kikowska et al 2012). The analyses of E. planum shoots agitated in the liquid MS media confirmed the presence of rosmarinic, chlorogenic, and caffeic acids in all the examined materials, but the content significantly increased after elicitation (Kikowska et al 2015). The content of two phenolic acids-rosmarinic acid and chlorogenic, was measured in shoots and roots of E. maritimum.…”

Section: Resultsmentioning

confidence: 74%

“…Although there are approximately 230-250 species of Eryngium in the world, only few have been introduced into in vitro cultures, namely E. foetidum (Arockisamy and Ignacimuthu 1998;Ignacimuthu et al 1999;Arockisamy et al 2002;Martin 2004;Chandrika et al 2011), E. planum (Kikowska et al 2012(Kikowska et al , 2015Thiem et al 2013), E. maritimum (Kikowska et al 2014), E. campestre (Kikowska et al 2016), and E. viviparum (Ayuso et al 2019).…”

Section: Resultsmentioning

confidence: 99%

“…The qualitative and quantitative analyses of phenolic acids in biomass from in vitro cultures were also conducted for other Eryngium species (Kikowska et al 2012(Kikowska et al , 2014(Kikowska et al , 2015(Kikowska et al ,2016Thiem et al 2013). The organs of the intact plants and in vitro propagated plantlets as well as callus and cell suspension of E. planum were rich in rosmarinic, chlorogenic and caffeic acids.…”

Section: Resultsmentioning

confidence: 99%

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Accumulation of valuable secondary metabolites: phenolic acids and flavonoids in different in vitro systems of shoot cultures of the endangered plant species—Eryngium alpinum L.

Kikowska

Thiem

Szopa

et al. 2020

Plant Cell Tiss Organ Cult

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In vitro cultures give the opportunity to perform the phytochemical studies on the protected species without harvesting the plant material from the natural environment. Shoots of Eryngium alpinum L. were multiplied on Murashige and Skoog (MS) medium in various systems, namely on the solid media and in two liquid cultures-stationary and agitated, as well as via regeneration from callus. The biomass increments were closely correlated with the number of shoots arising from one explant, which was connected with the supplementation of the culture media with the studied plant growth regulators. The methanolic extracts from shoots grown in the tested systems were subjected to phenolic acids and flavonoids qualitative and quantitative analysis. Biomass from in vitro shoot cultures accumulated from 19.59 to 32.95 times more phenolic acids [the total content ranged from 272.52 to 458.38 mg/100 g dry weight (DW)] and from 3.02 to 4.43 times more flavonoids (the total content ranged from 100.03 to 146.98 mg/100 g DW), depending on the culture system, than the extracts from basal leaves from the intact plant (13.91 and 33.16 mg/100 g DW, respectively). The phenolics present in shoot cultures include seven phenolic acids-3,4-dihydroxyphenylacetic, caftaric, caffeic, neochlorogenic, chlorogenic, isochlorogenic, and rosmarinic acids, and three flavonoids-isoquercetin, quercitrin and robinin. The best system for shoot proliferation resulting in the highest biomass growth and phenolic acids and flavonoids accumulation was solid culture on MS medium with BAP, IAA, and GA 3 (each 1.0 mg/l). The aim of this work was to check the effect of various culture systems (stationary and agitated, on solidified and in liquid media) on the production of phenolic compounds in E. alpinum shoots cultured in vitro. Key messageThis is the first report on phenolic acids and flavonoids estimation in Eryngium alpinum in vitro biomass from different culture systems.Keywords Alpine eryngo · Shoot multiplication · Phenolic acids · Flavonoids · HPLC-DAD analysis 1995). Some of the individuals are threatened by humansthe plant is collected for ornamental and commercial use and

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Accumulation of valuable secondary metabolites: phenolic acids and flavonoids in different in vitro systems of shoot cultures of the endangered plant species—Eryngium alpinum L.

Kikowska

Thiem

Szopa

et al. 2020

Plant Cell Tiss Organ Cult

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“…The research aimed at developing productive micropropagation systems with cytogenetic evaluation of in vitro regenerated plants, and stabilizing cell (callus, cell suspension cultures) and organ (shoots, roots) cultures of three Eryngium species. Moreover, the potential of the systems for production of the selected secondary metabolites and the assessment of selected biological activities were tested (Thiem at al., 2010;Thiem et al, 2011;Kikowska et al, 2012;Thiem et al, 2013;Kikowska et al, 2014a,b;Kikowska et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity

Kikowska¹,

Thiem²,

Śliwińska³

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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An efficient micropropagation protocol for production of genetically uniform clones of Eryngium campestre L. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro-and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg l -1 benzyladenine (BA) and 0.1 mg l -1 indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteria Staphylococcus aureus, as well as dermatophytes Trichophyton mentagrophytes and T. rubrum.

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“…Furthermore, addition of Jasmonic acid or methyl jasmonate to Mentha piperita suspension cultures resulted in high accumulation of phenolic compounds (Krzyzanowska et al 2012). Recently, Kikowska et al (2015) reported the optimum condition of elicitation for cultivation of shoots of Eryngium planum in liquid MS media in the presence of 100 μM MeJA for 48 h. This resulted in an approximately 4.5-fold increase in rosmarinic, chlorogenic and caffeic acid in plant material compared with the control. The results in the present study revealed that addition of jasmonic acid caused an increase in caffeic acid and total sugar content of the cultures used.…”

Section: Discussionmentioning

confidence: 99%

Active compounds and biological activity of in vitro cultures of some Echinacea purpurea varieties

2018

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Background: Plant cell cultures have developed rapidly in recent years for the synthesis of selected natural products. This study was conducted to evaluate the effect of yeast extract, jasmonic acid, phenylalanine, and tyrosine on total caffeic acid and polysaccharide production in callus cultures of two Echinacea varieties, namely Double Decker and Rubinstern. The biological activities (antioxidant, antimicrobial and cytotoxic activities) of different Echinacea extracts were also evaluated. Results: Data revealed the effect of yeast extract on calli of the two varieties. Double Decker calli recorded maximum caffeic acid derivatives and total hydrolysable sugars after 30 days of cultivation using 1.5 g/l and 1.0 g/l yeast extract respectively whereas, in Rubinstern calli, the maximum caffeic acid derivatives and total hydrolysable sugars were recorded at 1.0 g/l after 30 days. Using 50 mM jasmonic acid, Double Decker calli recorded maximum values of caffeic acid derivatives and total hydrolysable sugars after 15 days. In Rubinstern calli, caffeic acid derivatives and total hydrolysable sugar recorded maximum values after 30 days at 200 mM jasmonic acid and after 15 days at 50 mM jasmonic acid, respectively. In Double Decker variety, caffeic acid derivatives and total hydrolysable sugars recorded maximum values at 100 mg/l phenylalanine. Rubinstern calli recorded maximum value of caffeic acid derivatives at 100 mg/l phenylalanine and total hydrolysable sugars at 50 mg/l phenylalanine. As for tyrosine, maximum values of caffeic acid derivatives and total hydrolysable sugars recorded at 150 mg/l with Double Decker calli. Rubinstern calli recorded maximum value of caffeic acid derivatives and total hydrolysable sugars at 150 and 50 mg/l tyrosine, respectively. The biological activities of the different Echinacea extracts showed that maximum antioxidant activity (89.2%) was recorded with Rubinstern calli. Also, the maximum value of cell death (78.2%) was observed with the extract of Rubinstern calli. For antibacterial activity, most extracts showed inhibitory effect against Bacillus subtilis and Staphylococcus aureus growth. Conclusion: Both elicitors (yeast extract and jasmonic acid) and precursors (phenylalanine and tyrosine) have a clear effect on natural products of the two Echinacea varieties. The investigated Echinacea extracts (in vitro and in vivo plants and calli of the two varieties) showed moderate activity against tested microbial strains.

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Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures

Cited by 27 publications

References 16 publications

Accumulation of valuable secondary metabolites: phenolic acids and flavonoids in different in vitro systems of shoot cultures of the endangered plant species—Eryngium alpinum L.

Accumulation of valuable secondary metabolites: phenolic acids and flavonoids in different in vitro systems of shoot cultures of the endangered plant species—Eryngium alpinum L.

Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity

Active compounds and biological activity of in vitro cultures of some Echinacea purpurea varieties

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