Methyl jasmonate and salicylic acid elicit indole alkaloid production and modulate antioxidant defence and biocidal properties in Rauvolfia serpentina Benth. ex Kurz. in vitro cultures

Dey, Abhijit; Nandy, Samapika; Nongdam, Potshangbam; Tikendra, Leimapokpam; Mukherjee, Anuradha; Mukherjee, Souryadeep; Pandey, Devendra Kumar

doi:10.1016/j.sajb.2020.07.020

Cited by 34 publications

(11 citation statements)

References 64 publications

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“…Similarly, compared to bacterial auxin, seed germination period were prolonged to 55 days in control synthetic media MS +BAP (1 mg/L) and NAA (1 mg/L). Similar results were also reported on PLB and callus formation using the combination of auxin (NAA) and cytokinin (BAP) (Pant and Swar, 2011;Dey et al, 2020). Thus, current study also proved that bacterial auxin supplemented with 1mg/L in tissue culture MS media may be a substitute for synthetic auxin.…”

Section: B a Figuresupporting

confidence: 88%

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

et al. 2022

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Root-associated bacteria strongly affect plant growth and development by synthesizing growth regulators and stress-relieving metabolites. The present study is mainly focused on assessing aerial root-associated bacteria of Rhynchostylis retusa (L.) Blume is an endemic epiphytic orchid responsible for auxin production and influencing plant growth. A bacterial isolate, Microbacterium testaceum Y411, was found to be the most active producer of indole-3-acetic acid (IAA). The maximum IAA production (170µg/mL) was recorded with the bacterium at optimum process parameters such as pH 7, temperature 30°C, and tryptophan 1000 µg/mL in a culture medium for 48 h. The extracted auxin was purified and analyzed by FT-IR, HPLC, and HR-MS, indicating bacterial auxin has a similar mass value to 4-chloroindole-3-acetic acid auxin. Furthermore, the bacterial auxin was tested on in vitro propagation of orchid, Cymbidium aloifolium, and 90% seed germination was recorded in Murashige and Skoog’s medium supplemented with bacterial auxin. The novel results obtained in this study are used for agricultural applications and the Microbacterium testaceum Y411 is a valuable biotechnological resource for a natural auxin.

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Section: B a Figuresupporting

confidence: 88%

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

et al. 2022

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“…A similar trend in proline content enhancement in stress environments was noted earlier in several studies [ 43 , 44 ]. Abiotic and biotic stress stimulated reactive oxygen species (ROS) production and increased antioxidant activity, such as CAT, SOD and APX, in response to oxidative damage [ 4 ]. Upon the excessive production of ROS, SOD activity is enhanced, which releases hydrogen peroxide (H 2 O 2 ) as a by-product.…”

Section: Discussionmentioning

confidence: 99%

“…Different classes of secondary metabolites (SMs), such as phenolic, flavonoids, alkaloids, triterpenoids, saponins and steroids, are of great human interest as these serve as potent therapeutic drugs in the pharmaceutical industry [ 3 ]. These plant secondary metabolites are now being extensively utilized in herbal, cosmetic and nutraceutical formulations [ 4 ]. However, the over-exploitation of plants has led to a drastic decline of biodiversity [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Elicitation Induced α-Amyrin Synthesis in Tylophora indica In Vitro Cultures and Comparative Phytochemical Analyses of In Vivo and Micropropagated Plants

Mamgain,

Mujib,

Bansal

et al. 2023

Plants

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Tylophora indica (Burm. f.) Merrill is an endangered medicinal plant that possesses various active agents, such as tylophorinine, kaempferol, quercetin, α-amyrin and beta-sitosterol, with multiple medicinal benefits. α-amyrin, a triterpenoid, is widely known for its antimicrobial, anti-inflammatory, gastroprotective and hepatoprotective properties. In this study, we investigated the metabolite profiling of tissues and the effects of cadmium chloride and chitosan on in vitro accumulation of alkaloids in T. indica. First, the callus was induced from the leaf in 2,4-D-, NAA- and/or BAP-fortified MS medium. Subsequent shoot formation through organogenesis and in vitro roots was later induced. Gas chromatography–mass spectrometry (GC–MS)-based phytochemical profiling of methanolic extracts of in vivo and in vitro regenerated plants was conducted, revealing the presence of the important phytocompounds α-amyrin, lupeol, beta-sitosterol, septicine, tocopherol and several others. Different in vitro grown tissues, like callus, leaf and root, were elicited with cadmium chloride (0.1–0.4 mg L−1) and chitosan (1–50 mg L−1) to evaluate the effect of elicitation on α-amyrin accumulation, measured with high-performance thin layer chromatography (HPTLC). CdCl2 and chitosan showed improved sugar (17.24 and 15.04 mg g−1 FW, respectively), protein (10.76 and 9.99 mg g−1 FW, respectively) and proline (7.46 and 7.12 mg g−1 FW), especially at T3 (0.3 and 25 mg L−1), in the leaf as compared to those of the control and other tissues. The antioxidant enzyme activities were also evaluated under an elicitated stress situation, wherein catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) displayed the highest activities in the leaf at T4 of both of the two elicitors. The α-amyrin yield was quantified with HPTLC in all tested tissues (leaf, callus and root) and had an Rf = 0.62 at 510 nm wavelength. Among all the concentrations tested, the T3 treatment (0.3 mg L−1 of cadmium chloride and 25 mg L−1 of chitosan) had the best influence on accumulation, irrespective of the tissues, with the maximum being in the leaf (2.72 and 2.64 μg g−1 DW, respectively), followed by the callus and root. Therefore, these results suggest future opportunities of elicitors in scaling up the production of important secondary metabolites to meet the requirements of the pharmaceutical industry.

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“…Recent investigations on the in vitro production of Sarphagandha chemicals and components have yielded encouraging findings. Cell cultures of R. serpentina, the plant from which Sarphagandha is derived, were used to efficiently produce ajmalicine, serpentine, and catharanthine 76 . According to one study published in Springer, adding elicitors to cell cultures, such as yeast extract or salicylic acid, significantly enhanced the synthesis of ajmalicine and serpentine 77 .…”

Section: The Endangered Medicinal Plants: In Vitro Propagationmentioning

confidence: 99%

Venom Vanquish: A Comprehensive Review on Ethnobotany and Phytochemistry of Significant Traditional Medicinal Plants

T. Dhanya,

Kavinila,

David Paul Raj

2024

JNR

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Snakebite is a significant public health concern in many parts of the world, particularly in regions where venomous snakes are prevalent. Snakebites can range from mild to life-threatening, depending on the species of snake, the amount of venom injected, and the location of the bite. Globally, an estimated 5 million people are affected by snake bites each year, according to recent statistics. Since ancient times, traditional medicinal plants have been used to cure a wide range of illnesses, including snake bites. Secondary metabolites contained in plants were efficient in reducing the effects of snake venom. Snake venom is a potent toxin that can cause severe damage to the human body, affecting the nervous system, cardiovascular system, or both. Symptoms can range from mild swelling to life-threatening paralysis, respiratory failure, and hemorrhage. Enzyme activity is inhibited by flavonoids, alkaloids, and tannins, which additionally hinder blood from clotting and lessen inflammation. Treatments for snake bites that are more beneficial and less hazardous could result from the isolation and synthesis of natural drugs. With the use of cutting-edge scientific methods, we can maximize their potential and use their secondary metabolites to develop novel medications and therapies. The study of these plants and their potential uses in contemporary medicine requires more funding and investigation. This will advance our knowledge of these plants and help us create cures for some of the most lethal diseases.

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Methyl jasmonate and salicylic acid elicit indole alkaloid production and modulate antioxidant defence and biocidal properties in Rauvolfia serpentina Benth. ex Kurz. in vitro cultures

Cited by 34 publications

References 64 publications

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

Auxin biosynthesis by Microbacterium testaceum Y411 associated with orchid aerial roots and their efficacy in micropropagation

Elicitation Induced α-Amyrin Synthesis in Tylophora indica In Vitro Cultures and Comparative Phytochemical Analyses of In Vivo and Micropropagated Plants

Venom Vanquish: A Comprehensive Review on Ethnobotany and Phytochemistry of Significant Traditional Medicinal Plants

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