Methyl Green-Pyronin with Hematoxylin and Orange G for the Identification of Inflammatory Cells in Tissue Sections

Abstract

Methyl green-pyronin is a notoriously difficult stain to reproduce. Although very useful in detecting cells containing substantial amounts of RNA, it is of limited use in broader problems of cell identification. By careful standardization of the proportions of methyl green to pyronin and combination of these stains with hematoxylin to enhance nuclear contrast and with orange G to improve connective tissue staining, it was possible to produce a consistently reliable staining preparation in which it is possible to identify all the component cells of a mixed inflammatory infiltrate in routine paraffin sections.

Multiple Myeloma 1 Transcription Factor Is Superior to CD138 as a Marker of Plasma Cells in Endometrium

“…This stain, aside from being nonspecific, is difficult to reproduce, and even sometimes difficult to interpret. 9 Molecular polymerase chain reaction assay for detection of the common microbial pathogens has shown to be comparable with other modalities including endometrial bacterial cultures and histopathology 10 while vaginal and endocervical bacterial cultures have low yield and poor reliability. 11 The first attempt to use an immunoperoxidase stain was made by Crum et al, 6 who used anti-immunoglobulin (Ig) G to detect plasma cells.…”

Intraepithelial plasma cells in the avian copulatory organ of two tinamou species: quantitative variation during the breeding season

“…For light microscopy, fragments were embedded in glycol methacrylate, sectioned at 5-7 mm and stained with toluidine blue-sodium borate and methyl green-pyronin. The latter stain is a quick and effective method used to demonstrate plasma cells in tissues (Opstad 1959;Ahlqvist and Anderson 1972;Linder et al 1977;Rideout et al 1992). For ultrastructural studies, fragments were postfixed in 1.0% (w/v) osmium tetroxide in 0.1 M cacodylate buffer (pH 7.4) and embedded in Polybed 812 resin.…”