Methotrexate Polyglutamates Analysis by Chromatography Methods in Biological Matrices: A Review

Zhang, Yiming; Sun, Liyu; Zhao, Libo; Wang, Xiaoling; Zhao, Zhigang; Mei, Shenghui

doi:10.2116/analsci.21r001

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Among these methods, liquid chromatography (LC) coupled with tandem MS (LC-MS/MS) has emerged as a potential tool for rapid, sensitive, and cost-effective method for therapeutic drug monitoring of MTX. 26 27 However, there are very few commercially available kits for the LC-MS/MS-based assays and application note of these kits may not be available for all LC-MS/MS instruments for application in routine patient care for therapeutic drug monitoring of MTX in clinical laboratories particularly in India.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Method for Determination of Serum Methotrexate Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Its Application in Therapeutic Drug Monitoring

et al. 2023

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Objectives Methotrexate (MTX) has anticancer therapeutic potential with multiple doses-related adverse effects and toxicities. Immunoassays for therapeutic monitoring of serum MTX have their own limitations. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) is considered as the reference method; however, commercially availability of them is limited. We aimed to adapt/develop an in-house LC-MS/MS method for therapeutic monitoring of serum MTX. Materials and Methods Serum protein precipitation was performed using acetonitrile–water containing 250 μM solution of aminoacetophenone as internal standard (IS). Chromatographic separation was achieved on a C18 column with mobile phase of 0.1% solution of formic acid (solvent A) and acetonitrile (solvent B) at a flow rate of 0.4 mL/min. MS was performed under positive ion mode with mass transition for MTX and IS as m/z 455.1→308.1 and 136.2→94.1, respectively. The method was validated by following Bioanalytical Method Validation Guidance for Industry, 2018 and applied on leukemia patients' samples on MTX therapy. Results The correlation coefficient of eight serially diluted calibration standards of 0.09 to 12.5 μM was >0.99 and had linearity with > 95% precision and accuracy at analytical quality control levels. The lower limit of MTX quantification achieved was 0.09 μM with good intensity and sharp peak as compared with blank sample. The total run time of the assay was 5 minutes. The serum MTX levels obtained by this method in leukemia patients exhibited clinical correlation and an excellent agreement with commercial immunoassay used in parallel. Conclusion We were able to develop a rapid, sensitive, and cost-effective LC-MS/MS method suitable for therapeutic drug monitoring of MTX in routine clinical diagnostic laboratories.

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Section: Introductionmentioning

confidence: 99%

A Rapid Method for Determination of Serum Methotrexate Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Its Application in Therapeutic Drug Monitoring

et al. 2023

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Development and validation of a bioanalytical method for the quantification of methotrexate from serum and capillary blood using volumetric absorptive microsampling (VAMS) and on-line solid phase extraction (SPE) LC-MS

Opitz,

Fobker,

Fabian

et al. 2024

Journal of Chromatography A

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Nonaqueous Capillary Zone Electrophoresis for Polyesters and Polyamides Using Hexafluoroisopropyl Alcohol as Electrophoretic Medium

et al. 2022

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Nonaqueous capillary zone electrophoresis (NACZE) utilizing cationic reagents as an electrophoresis promoter has the potential for separating water-insoluble synthetic polymers and that with tetrahydrofuran based electrophoretic media containing cationic reagents has been reported. In this study, NACZE using hexafluoroisopropanol (HFIP)-based media was developed for separating polyesters and polyamides, which were insoluble in tetrahydrofuran. Nonaqueous media of HFIP containing 400 mM cetyltrimethylammonium chloride (CTAC) and 50 mM trihexyl(tetradecyl)phosphonium chloride (P66614Cl) were used, and it was found that hydrophobic P66614 cation enhanced the electrophoresis of both polyesters and polyamides compared with cetyltrimethylammonium cation. The media containing CTAC resulted in partially separated poly(ethylene terephthalate)/poly(hexamethylene terephthalate) and poly(ethylene terephthalate)/poly(ethylene naphthalate), but P66614Cl failed to separate them. By contrast, nylon 6I and nylon 6/6I were separated with the P66614Cl media, whereas they were co-eluted in the media with CTAC.

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Methotrexate Polyglutamates Analysis by Chromatography Methods in Biological Matrices: A Review

Cited by 3 publications

References 53 publications

A Rapid Method for Determination of Serum Methotrexate Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Its Application in Therapeutic Drug Monitoring

A Rapid Method for Determination of Serum Methotrexate Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry and Its Application in Therapeutic Drug Monitoring

Development and validation of a bioanalytical method for the quantification of methotrexate from serum and capillary blood using volumetric absorptive microsampling (VAMS) and on-line solid phase extraction (SPE) LC-MS

Nonaqueous Capillary Zone Electrophoresis for Polyesters and Polyamides Using Hexafluoroisopropyl Alcohol as Electrophoretic Medium

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