Methods used in the United Kingdom for the culture of micro-organisms from blood.

Brown, D L; Perry, S.

doi:10.1136/jcp.45.6.468

Cited by 13 publications

(5 citation statements)

References 32 publications

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“…Previous reports have produced sensitivities varying from 12.8%l1 to 77.3%.8 Staphaurex's previously quoted high sensitivity may explain why four UK laboratories in a 1992 survey opted to use a staphylococcal latex kit for the direct identification of positive blood cultures. 3 The design of previous clinical evaluations of commercial slide agglutination kits has been extremely variable. Some workers have used seeded blood cultures, others clinical cultures.…”

Section: Discussionmentioning

confidence: 99%

“…2 Though the early distinction between Staphylococcus aureus and coagulase negative staphylococci (CNS) may be helpful in initiating appropriate antimicrobial treatment, few laboratories in the UK proceed to rapid identification tests if staphylococci are suspected in blood culture media. 3 Those who do test directly usually prefer a tube coagulase method to detect free coagulase rather than a slide agglutination test which detects clumping factor or protein A, or both. 3 Commerical slide agglutination kits for the identification of S aureus are widely used as they are particularly convenient and technically simple.…”

mentioning

confidence: 99%

“…3 Those who do test directly usually prefer a tube coagulase method to detect free coagulase rather than a slide agglutination test which detects clumping factor or protein A, or both. 3 Commerical slide agglutination kits for the identification of S aureus are widely used as they are particularly convenient and technically simple. However, they have been principally designed for isolates grown on solid culture media.4 Previous reports have suggested that sensitivity, though not specificity, may be reduced if slide agglutination tests are performed directly from blood culture broths.…”

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confidence: 99%

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Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.

Cooke¹,

Jenkins²

1997

Journal of Clinical Pathology

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.

Cooke¹,

Jenkins²

1997

Journal of Clinical Pathology

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“…Aliquot inoculation was performed in the following selective nutrient media: (i) meat-peptone agar (MPA) for aerobic and facultatively anaerobic bacteria, (ii) BAIRD-PARKER-Agar (Merck, Germany) for Staphylococcus spp., (iii) KF-Streptococcus agar (Merck, Germany) for Streptococcus spp., (iv) MRSA (MRSA, HiMedia, India) for Lactobacillus spp., (v) Bifi dum agar (HiMedia, India) for Bifi dobacterium spp., (vi) Endo (HiMedia, India) for coliform bacteria, (vii) Sabouraud agar (HiMedia, India) for microscopic fungi, and (viii) Pseudomonas agar (HiMedia, India) for Pseudomonas spp. [22][23][24]. Aft er 24 h of incubation, we recorded preliminary data, and aft er 48 h, -the fi nal ones.…”

Section: Results Th E Formation Of Bacterial Vaginitis In the Balb/c ...mentioning

confidence: 99%

Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

et al. 2023

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The purpose of this study was to investigate the antimicrobial and immunomodulatory action of a probiotic composition of Bacillus subtilis and B. megatherium strains (UnicaUro, Sirion (Ukraine)) for experimental bacterial vaginitis. Methods. Experimental studies were conducted on female BALB/c mice; we used Staphylococcus aureus strain B-918 (ATCC 6538) to induce bacterial vaginitis. The strain was vaginally introduced into mice before treatment with probiotic bacteria. In the vagina of mice, aerobic and optionally anaerobic bacteria, including representatives of the genera Staphylococcus, Streptococcus, Lactobacillus, Bifidobacterium, Pseudomonas, coliform bacteria, and microscopic fungi were identified in different periods of observation using generally accepted microbiological methods. Serum antibody titer to S. aureus was determined by the bacterial agglutination reaction. The phagocytic activity and oxygen-dependent bactericidal activity of peritoneal exudate macrophages (PEM) were evaluated using generally accepted immunological methods. Results. The formation of bacterial vaginitis in the BALB/c mice line infected with S. aureus B-918 (ATCC 6538) was evidenced by the appearance of external clinical manifestations of the infectious and inflammatory process against the background of the increased number of aerobic and optionally anaerobic microorganisms, including representatives of the genus Staphylococcus and Streptococcus, microscopic fungi, and decreased number of lactobacilli in different observation periods. The probiotic introduction to mice with bacterial vaginitis led to a dynamic change in the vaginal microbiota: the number of aerobic and optionally anaerobic microorganisms decreased, primarily due to the normalization of the number of representatives of Staphylococcus genus accompanied by a decrease in the antibody titer to staphylococcus in the blood serum. The effective therapeutic action of the probiotic was confirmed by the gradual disappearance of the external clinical signs of the infectious-inflammatory process in the vagina against the background of the functional activity of PEM. Conclusions. The probiotic composition of B. subtilis and B. megatherium (UnicaUro, Sirion, Ukraine) is a promising antimicrobial formulation that may be used in the treatment of bacterial vaginitis; however, further studies are required to confirm its therapeutic, antimicrobial, and immunomodulatory efficacy.

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“…To determine the spectrum of microbiota, aliquots from the vagina and feces were plated on eight nutrient media, namely: meat-peptone agar (MPA) -medium for cultivation of aerobic and optional anaerobic microorganisms; BAIRD-PARKER-Agar (Merck, Germany) -selective medium for staphylococci; BAIRD-PARKER-Agar with gentami-cin in concentration of 15 μg/mL -selective medium for isolation of S. aureus 8325-4 strain; KF-Streptococcus agar (Merck, Germany)selective medium for streptococci, MRSA (HiMedia, India) -selective medium for lactobacilli; BA (HiMedia, India) -selective medium for bifidobacteria; ENDO (HiMedia, India) -selective medium for coliform bacteria; Sabouraud agar (HiMedia, India) -selective medium for microscopic fungi. After cultivation at 37 °C for 24 hours, the number of colonies per petri dish was calculated, given that one such colony was grown out of one bacterium (Brown & Perry, 1992).…”

Section: Methodsmentioning

confidence: 99%

Prophylactic effect of lactobacilli and bifidobacteria probiotic strains on experimental bacterial vaginitis

et al. 2019

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The objective of the study was determining the prophylactic effect of Lactobacillus casei IMV B-7280, L. acidophilus IMV B-7279, L. delbrueckii subsp. bulgaricus IMV B-7281, Bifidobacterium animalis VKL and B. animalis VKB probiotic strains on experimental vaginitis in BALB/c mice induced by Staphylococcus aureus 8325-4. The infection with S. aureus 8325-4 caused an imbalance of microbiota in the vagina and intestine, as evidenced by an increase in the number of opportunistic microorganisms and a decrease in the amount of lactobacilli and bifidobacteria. L. casei IMV B-7280, B. animalis VKL and B. animalis VKB probiotic strains altered the microbiota spectrum of the vagina and intestine of Staphylococcus-infected mice: the amount of Lactobacillus and bifidobacteria increased with the reduction of the number of opportunistic microorganisms. Also under the influence of these strains, the normalization of the microbiota spectrum typical for vagina and intestine was observed in different periods of observation – in the intestines of mice the number of coliform bacteria increased, the number of microscopic fungi, streptococci and staphylococci decreased; in the vagina, the number of coliform bacteria and microscopic fungi decreased, the number of streptococci normalized. Rapid elimination of S. aureus 8325-4 from the vagina and prevention of the spread of infection to the intestine were observed after use of probiotics. Preventive effect of L. acidophilus IMV B-7279 and L. delbrueckii subsp. bulgaricus IMV B-7281 for bacterial vaginitis in mice was less effective. So, the target probiotic strains L. casei IMV B-7280, B. animalis VKL and B. animalis VKB are promising for the creation of highly effective novel probiotic drugs that can be used for directed prevention of infectious and inflammatory diseases of the genitourinary system caused by pathogenic and opportunistic microorganisms.

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Methods used in the United Kingdom for the culture of micro-organisms from blood.

Abstract: Clinical Microbiology

Cited by 13 publications

References 32 publications

Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.

Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.

Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

Prophylactic effect of lactobacilli and bifidobacteria probiotic strains on experimental bacterial vaginitis

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