Comparison of commercial slide agglutination kits with a tube coagulase test for the rapid identification of Staphylococcus aureus from blood culture.

Characterization of a Protective Monoclonal AntibodyRecognizing Staphylococcus aureus MSCRAMM ProteinClumping FactorA

“…In a flow cytometry assay, MAb 12-9 effectively recognized every S. aureus isolate analyzed, providing strong evidence that the native ClfA epitope is highly conserved. These data are supportive of previous reports that indicate that the presence of the clfA gene (5,34) and ClfA-mediated fibrinogen binding (9,36,50) is a trait conserved in a vast majority of S. aureus strains. While these data may be semiquantitative in nature, it is important to note that the flow cytometry analysis reveals the percentage of positively staining cells at one point in the time of S. aureus isolate cell growth.…”

Comparison of four methods for rapid identification of Staphylococcus aureus directly from BACTEC 9240 blood culture system

“…They also found no significant difference between the test results read at 2, 6, and 24 h. Test sensitivities ranged from 87 to 92% for 2 and 24 h with specificities of 100%. [8] In our study the sensitivity of TCT was similar or slightly higher than those reported previously and the performance of the test results read at 2, 4, and 24 h were not statistically different.…”

“…[6] However in recent reports, these tests showed poor sensitivities when performed directly from blood culture broth (BCB) and it is recommended that negative results should be confirmed. [8,11] Several nucleic acid based amplification methods have been reported for the identification of S. aureus. The same-day differentiation is the advantage of molecular tests but they are expensive and needs qualified personnel.…”

“…[7][8][9][10] Other alternative methods are slide agglutination tests for clumping factor, protein-A, and other surface antigens. [6] However in recent reports, these tests showed poor sensitivities when performed directly from blood culture broth (BCB) and it is recommended that negative results should be confirmed.…”

Correspondence

“…There have been several reports of different rapid tests that have been evaluated that have shown varied sensitivity and specificity [3,4]. RAPIDEC staph TM (bioMérieux, Marcy-l9Etoile, France) is a 2-h test for the presumptive identification of S. aureus directly from blood culture bottles growing gram-positive cocci [2,5,6].…”