Comparison of four methods for rapid identification of Staphylococcus aureus directly from BACTEC 9240 blood culture system

Ozen, NS; Öğünç, Dilara; Mutlu, Derya; Öngüt, Gözde; Baysan, BO; Günseren, Filiz

doi:10.4103/0255-0857.76523

Cited by 9 publications

(2 citation statements)

References 19 publications

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“…A number of simple direct tests have been described. Examples are detection of Staphylococcus aureus by direct tube coagulase test for detection of Staphylococcus aureus (271, 272), latex agglutination tests (273) and immunochromatographic tests (274), and the recent development of lateral flow assays for Burkholderia pseudomallei (275) and Salmonella species (276).…”

Section: Challengesmentioning

confidence: 99%

Best Practices of Blood Cultures in Low- and Middle-Income Countries

et al. 2019

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Bloodstream infections (BSI) have a substantial impact on morbidity and mortality worldwide. Despite scarcity of data from many low- and middle-income countries (LMICs), there is increasing awareness of the importance of BSI in these countries. For example, it is estimated that the global mortality of non-typhoidal Salmonella bloodstream infection in children under 5 already exceeds that of malaria. Reliable and accurate diagnosis of these infections is therefore of utmost importance. Blood cultures are the reference method for diagnosis of BSI. LMICs face many challenges when implementing blood cultures, due to financial, logistical, and infrastructure-related constraints. This review aims to provide an overview of the state-of-the-art of sampling and processing of blood cultures, with emphasis on its use in LMICs. Laboratory processing of blood cultures is relatively straightforward and can be done without the need for expensive and complicated equipment. Automates for incubation and growth monitoring have become the standard in high-income countries (HICs), but they are still too expensive and not sufficiently robust for imminent implementation in most LMICs. Therefore, this review focuses on “manual” methods of blood culture, not involving automated equipment. In manual blood cultures, a bottle consisting of a broth medium supporting bacterial growth is incubated in a normal incubator and inspected daily for signs of growth. The collection of blood for blood culture is a crucial step in the process, as the sensitivity of blood cultures depends on the volume sampled; furthermore, contamination of the blood culture (accidental inoculation of environmental and skin bacteria) can be avoided by appropriate antisepsis. In this review, we give recommendations regarding appropriate blood culture sampling and processing in LMICs. We present feasible methods to detect and speed up growth and discuss some challenges in implementing blood cultures in LMICs, such as the biosafety aspects, supply chain and waste management.

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Section: Challengesmentioning

confidence: 99%

Best Practices of Blood Cultures in Low- and Middle-Income Countries

et al. 2019

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“…Automated image acquisition and machine learning–based approaches for automated Gram stain classifications have been explored, showing promising accuracy although still far from being able to perform as fully automated systems [ 20 ]. Early identification of important organisms such as Staphylococcus aureus directly from BC by coagulase testing has improved TAT and is inexpensive and easily incorporated into standard workflows [ 21 , 22 ]. Rapid antimicrobial susceptibility methods have been developed to reduce the time to results.…”

Section: Established Rapid Diagnostic Methodsmentioning

confidence: 99%

New Microbiological Techniques for the Diagnosis of Bacterial Infections and Sepsis in ICU Including Point of Care

Peri

Stewart

Hume

et al. 2021

Curr Infect Dis Rep

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Purpose of Review The aim of this article is to review current and emerging microbiological techniques that support the rapid diagnosis of bacterial infections in critically ill patients, including their performance, strengths and pitfalls, as well as available data evaluating their clinical impact. Recent Findings Bacterial infections and sepsis are responsible for significant morbidity and mortality in patients admitted to the intensive care unit and their management is further complicated by the increase in the global burden of antimicrobial resistance. In this setting, new diagnostic methods able to overcome the limits of traditional microbiology in terms of turn-around time and accuracy are highly warranted. We discuss the following broad themes: optimisation of existing culture-based methodologies, rapid antigen detection, nucleic acid detection (including multiplex PCR assays and microarrays), sepsis biomarkers, novel methods of pathogen detection (e.g. T2 magnetic resonance) and susceptibility testing (e.g. morphokinetic cellular analysis) and the application of direct metagenomics on clinical samples. The assessment of the host response through new “omics” technologies might also aid in early diagnosis of infections, as well as define non-infectious inflammatory states. Summary Despite being a promising field, there is still scarce evidence about the real-life impact of these assays on patient management. A common finding of available studies is that the performance of rapid diagnostic strategies highly depends on whether they are integrated within active antimicrobial stewardship programs. Assessing the impact of these emerging diagnostic methods through patient-centred clinical outcomes is a complex challenge for which large and well-designed studies are awaited.

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Bacterial pathogens associated with community-acquired pneumonia in children aged below five years

et al. 2016

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Comparison of four methods for rapid identification of Staphylococcus aureus directly from BACTEC 9240 blood culture system

Abstract: Our results suggested that 2 h TCT was found to be simple and inexpensive method for the rapid identification of S. aureus directly from positive blood cultures.

Cited by 9 publications

References 19 publications

Best Practices of Blood Cultures in Low- and Middle-Income Countries

Best Practices of Blood Cultures in Low- and Middle-Income Countries

New Microbiological Techniques for the Diagnosis of Bacterial Infections and Sepsis in ICU Including Point of Care

Bacterial pathogens associated with community-acquired pneumonia in children aged below five years

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